Phytoplasma SAP11 effector destabilization of TCP transcription factors differentially impact development and defence of Arabidopsis versus maize

English version

Autoři: Pascal Pecher ^aff001; Gabriele Moro ^aff001; Maria Cristina Canale ^aff001; Sylvain Capdevielle ^aff001; Archana Singh ^aff001; Allyson MacLean ^aff001; Akiko Sugio ^aff001; Chih-Horng Kuo ^aff003; Joao R. S. Lopes ^aff002; Saskia A. Hogenhout ^aff001
Působiště autorů: John Innes Centre, Department of Crop Genetics, Norwich Research Park, Norwich, United Kingdom ^aff001; Luiz de Queiroz College of Agriculture, Department of Entomology and Acarology, University of São Paulo, Piracicaba, Brazil ^aff002; Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan ^aff003
Vyšlo v časopise: Phytoplasma SAP11 effector destabilization of TCP transcription factors differentially impact development and defence of Arabidopsis versus maize. PLoS Pathog 15(9): e32767. doi:10.1371/journal.ppat.1008035
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.ppat.1008035

Souhrn

Phytoplasmas are insect-transmitted bacterial pathogens that colonize a wide range of plant species, including vegetable and cereal crops, and herbaceous and woody ornamentals. Phytoplasma-infected plants often show dramatic symptoms, including proliferation of shoots (witch’s brooms), changes in leaf shapes and production of green sterile flowers (phyllody). Aster Yellows phytoplasma Witches’ Broom (AY-WB) infects dicots and its effector, secreted AYWB protein 11 (SAP11), was shown to be responsible for the induction of shoot proliferation and leaf shape changes of plants. SAP11 acts by destabilizing TEOSINTE BRANCHED 1-CYCLOIDEA-PROLIFERATING CELL FACTOR (TCP) transcription factors, particularly the class II TCPs of the CYCLOIDEA/TEOSINTE BRANCHED 1 (CYC/TB1) and CINCINNATA (CIN)-TCP clades. SAP11 homologs are also present in phytoplasmas that cause economic yield losses in monocot crops, such as maize, wheat and coconut. Here we show that a SAP11 homolog of Maize Bushy Stunt Phytoplasma (MBSP), which has a range primarily restricted to maize, destabilizes specifically TB1/CYC TCPs. SAP11_MBSP and SAP11_AYWB both induce axillary branching and SAP11_AYWB also alters leaf development of Arabidopsis thaliana and maize. However, only in maize, SAP11_MBSP prevents female inflorescence development, phenocopying maize tb1 lines, whereas SAP11_AYWB prevents male inflorescence development and induces feminization of tassels. SAP11_AYWB promotes fecundity of the AY-WB leafhopper vector on A. thaliana and modulates the expression of A. thaliana leaf defence response genes that are induced by this leafhopper, in contrast to SAP11_MBSP. Neither of the SAP11 effectors promote fecundity of AY-WB and MBSP leafhopper vectors on maize. These data provide evidence that class II TCPs have overlapping but also distinct roles in regulating development and defence in a dicot and a monocot plant species that is likely to shape SAP11 effector evolution depending on the phytoplasma host range.

Klíčová slova:

Arabidopsis thaliana – Flowering plants – Genetically modified plants – Leaves – Maize – Sequence alignment – Transcription factors – Phytoplasmas

Zdroje

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