A fungal ABC transporter FgAtm1 regulates iron homeostasis via the transcription factor cascade FgAreA-HapX

English version

Autoři: Zhihui Wang ^aff001; Tianling Ma ^aff001; Yunyan Huang ^aff001; Jing Wang ^aff001; Yun Chen ^aff001; H. Corby Kistler ^aff003; Zhonghua Ma ^aff001; Yanni Yin ^aff001
Působiště autorů: State Key Laboratory of Rice Biology, Zhejiang University, Hangzhou, China ^aff001; Institute of Biotechnology, Key Laboratory of Molecular Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou, China ^aff002; United States Department of Agriculture, Agricultural Research Service, St. Paul, Minnesota, United States of America ^aff003
Vyšlo v časopise: A fungal ABC transporter FgAtm1 regulates iron homeostasis via the transcription factor cascade FgAreA-HapX. PLoS Pathog 15(9): e32767. doi:10.1371/journal.ppat.1007791
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.ppat.1007791

Souhrn

Iron homeostasis is important for growth, reproduction and other metabolic processes in all eukaryotes. However, the functions of ATP-binding cassette (ABC) transporters in iron homeostasis are largely unknown. Here, we found that one ABC transporter (named FgAtm1) is involved in regulating iron homeostasis, by screening sensitivity to iron stress for 60 ABC transporter mutants of Fusarium graminearum, a devastating fungal pathogen of small grain cereal crops worldwide. The lack of FgAtm1 reduces the activity of cytosolic Fe-S proteins nitrite reductase and xanthine dehydrogenase, which causes high expression of FgHapX via activating transcription factor FgAreA. FgHapX represses transcription of genes for iron-consuming proteins directly but activates genes for iron acquisition proteins by suppressing another iron regulator FgSreA. In addition, the transcriptional activity of FgHapX is regulated by the monothiol glutaredoxin FgGrx4. Furthermore, the phosphorylation of FgHapX, mediated by the Ser/Thr kinase FgYak1, is required for its functions in iron homeostasis. Taken together, this study uncovers a novel regulatory mechanism of iron homeostasis mediated by an ABC transporter in an important pathogenic fungus.

Klíčová slova:

DNA transcription – Fluorescence microscopy – Homeostasis – Mitochondria – Phosphorylation – Saccharomyces cerevisiae – Transcription factors – Transcriptional control

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