Is reliance on an inaccurate genome sequence sabotaging your experiments?

English version

Autoři: Rodrigo P. Baptista ^aff001; Jessica C. Kissinger ^aff001
Působiště autorů: Center for Tropical and Emerging Global Diseases, University of Georgia, Athens, Georgia, United States of America ^aff001; Institute of Bioinformatics, University of Georgia, Athens, Georgia, United States of America ^aff002; Department of Genetics, University of Georgia, Athens, Georgia, United States of America ^aff003
Vyšlo v časopise: Is reliance on an inaccurate genome sequence sabotaging your experiments?. PLoS Pathog 15(9): e1007901. doi:10.1371/journal.ppat.1007901
Kategorie: Pearls
doi: https://doi.org/10.1371/journal.ppat.1007901

Souhrn

Advances in genomics have made whole genome studies increasingly feasible across the life sciences. However, new technologies and algorithmic advances do not guarantee flawless genomic sequences or annotation. Bias, errors, and artifacts can enter at any stage of the process from library preparation to annotation. When planning an experiment that utilizes a genome sequence as the basis for the design, there are a few basic checks that, if performed, may better inform the experimental design and ideally help avoid a failed experiment or inconclusive result.

Klíčová slova:

Biology and life sciences – Computational biology – Genomic libraries – Genetics – Genomics – Genome analysis – Sequence assembly tools – Genome annotation – Molecular biology – Molecular biology techniques – DNA construction – DNA library construction – Genomic library construction – Molecular biology assays and analysis techniques – Library screening – Genomic library screening – Gene mapping – Organisms – Eukaryota – Protozoans – Parasitic protozoans – Cryptosporidium – Cryptosporidium parvum – Research and analysis methods – Database and informatics methods – Bioinformatics – Sequence analysis – Sequence alignment

Zdroje

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