A robust human norovirus replication model in zebrafish larvae

English version

Autoři: Jana Van Dycke ^aff001; Annelii Ny ^aff002; Nádia Conceição-Neto ^aff003; Jan Maes ^aff002; Myra Hosmillo ^aff004; Arno Cuvry ^aff001; Ian Goodfellow ^aff004; Tatiane C. Nogueira ^aff001; Erik Verbeken ^aff005; Jelle Matthijnssens ^aff003; Peter de Witte ^aff002; Johan Neyts ^aff001; Joana Rocha-Pereira ^aff001
Působiště autorů: KU Leuven–Department of Microbiology, Immunology and Transplantation, Rega Institute, Laboratory of Virology and Chemotherapy, Leuven, Belgium ^aff001; KU Leuven–Department of Pharmaceutical and Pharmacological Sciences, Laboratory for Molecular Biodiscovery, Leuven, Belgium ^aff002; KU Leuven–Department of Microbiology, Immunology and Transplantation, Rega Institute, Laboratory of Clinical and Epidemiological Virology, Leuven, Belgium ^aff003; University of Cambridge–Department of Pathology, Division of Virology, Addenbrooke's Hospital, Cambridge, United Kingdom ^aff004; KU Leuven–Department of Imaging & Pathology, Translational Cell & Tissue Research, Leuven, Belgium ^aff005; Global Virus Network (GVN), Centers of Excellence ^aff006
Vyšlo v časopise: A robust human norovirus replication model in zebrafish larvae. PLoS Pathog 15(9): e32767. doi:10.1371/journal.ppat.1008009
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.ppat.1008009

Souhrn

Human noroviruses (HuNoVs) are the most common cause of foodborne illness, with a societal cost of $60 billion and 219,000 deaths/year. The lack of robust small animal models has significantly hindered the understanding of norovirus biology and the development of effective therapeutics. Here we report that HuNoV GI and GII replicate to high titers in zebrafish (Danio rerio) larvae; replication peaks at day 2 post infection and is detectable for at least 6 days. The virus (HuNoV GII.4) could be passaged from larva to larva two consecutive times. HuNoV is detected in cells of the hematopoietic lineage and the intestine, supporting the notion of a dual tropism. Antiviral treatment reduces HuNoV replication by >2 log₁₀, showing that this model is suited for antiviral studies. Zebrafish larvae constitute a simple and robust replication model that will largely facilitate studies of HuNoV biology and the development of antiviral strategies.

Klíčová slova:

Biology and life sciences – Developmental biology – Life cycles – Larvae – Organisms – Eukaryota – Animals – Vertebrates – Fish – Osteichthyes – Viruses – RNA viruses – Caliciviruses – Norovirus – Microbiology – Virology – Viral replication – Medical microbiology – Microbial pathogens – Viral pathogens – Anatomy – Digestive system – Gastrointestinal tract – Physiology – Biological locomotion – Swimming – Research and analysis methods – Animal studies – Experimental organism systems – Model organisms – Zebrafish – Animal models – Extraction techniques – RNA extraction – Medicine and health sciences – Pathology and laboratory medicine – Pathogens

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