Signatures of oral microbiome in HIV-infected individuals with oral Kaposi's sarcoma and cell-associated KSHV DNA

English version

Autoři: Marion Gruffaz ^aff001; Tinghe Zhang ^aff002; Vickie Marshall ^aff003; Priscila Gonçalves ^aff004; Ramya Ramaswami ^aff004; Nazzarena Labo ^aff003; Denise Whitby ^aff003; Thomas S. Uldrick ^aff004; Robert Yarchoan ^aff004; Yufei Huang ^aff002; Shou-Jiang Gao ^aff001
Působiště autorů: Department of Molecular Microbiology and Immunology, Keck School of Medicine, University of Southern California, Los Angeles, United States of America ^aff001; Department of Electrical and Computer Engineering, University of Texas at San Antonio, San Antonio, Texas, United States of America ^aff002; Viral Oncology Section, AIDS and Cancer Virus Program, Leidos Biomedical Research Inc., Frederick National Laboratory for Cancer Research, Frederick, Maryland, United States of America ^aff003; HIV and AIDS Malignancy Branch, Center for Cancer Research, National Cancer Institute, Bethesda, Maryland, United States of America ^aff004; Fred Hutchinson Cancer Research Center, Seattle, Washington, United States of America ^aff005; Department of Epidemiology and Biostatistics, The University of Texas Health San Antonio, San Antonio, Texas, United States of America ^aff006; UPMC Hillman Cancer Center, Department of Microbiology and Molecular Genetics, University of Pittsburgh, Pittsburgh, Pennsylvania, United States of America ^aff007
Vyšlo v časopise: Signatures of oral microbiome in HIV-infected individuals with oral Kaposi's sarcoma and cell-associated KSHV DNA. PLoS Pathog 16(1): e32767. doi:10.1371/journal.ppat.1008114
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.ppat.1008114

Souhrn

Infection by Kaposi’s sarcoma-associated herpesvirus (KSHV) is necessary for the development of Kaposi’s sarcoma (KS), which most often develops in HIV-infected individuals. KS frequently has oral manifestations and KSHV DNA can be detected in oral cells. Numerous types of cancer are associated with the alteration of microbiome including bacteria and virus. We hypothesize that oral bacterial microbiota affects or is affected by oral KS and the presence of oral cell-associated KSHV DNA. In this study, oral and blood specimens were collected from a cohort of HIV/KSHV-coinfected individuals all previously diagnosed with KS, and were classified as having oral KS with any oral cell-associated KSHV DNA status (O-KS, n = 9), no oral KS but with oral cell-associated KSHV DNA (O-KSHV, n = 10), or with neither oral KS nor oral cell-associated KSHV DNA (No KSHV, n = 10). We sequenced the hypervariable V1-V2 region of the 16S rRNA gene present in oral cell-associated DNA by next generation sequencing. The diversity, richness, relative abundance of operational taxonomic units (OTUs) and taxonomic composition of oral microbiota were analyzed and compared across the 3 studied groups. We found impoverishment of oral microbial diversity and enrichment of specific microbiota in O-KS individuals compared to O-KSHV or No KSHV individuals. These results suggest that HIV/KSHV coinfection and oral microbiota might impact one another and influence the development of oral KS.

Klíčová slova:

Bacteria – HIV – Kaposi sarcoma – Kaposi's sarcoma-associated herpesvirus – Microbial taxonomy – Microbiome – Species diversity – T cells

Zdroje

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