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Structures of immature EIAV Gag lattices reveal a conserved role for IP6 in lentivirus assembly


Autoři: Robert A. Dick aff001;  Chaoyi Xu aff002;  Dustin R. Morado aff003;  Vladyslav Kravchuk aff004;  Clifton L. Ricana aff005;  Terri D. Lyddon aff005;  Arianna M. Broad aff001;  J. Ryan Feathers aff001;  Marc C. Johnson aff005;  Volker M. Vogt aff001;  Juan R. Perilla aff002;  John A. G. Briggs aff003;  Florian K. M. Schur aff004
Působiště autorů: Department of Molecular Biology and Genetics, Cornell University, Ithaca, New York, United States of America aff001;  Department of Chemistry and Biochemistry, University of Delaware, Newark, Delaware, United States of America aff002;  Structural Studies Division, Medical Research Council Laboratory of Molecular Biology, Cambridge, United Kingdom aff003;  Institute of Science and Technology Austria, Klosterneuburg, Austria aff004;  Department of Molecular Microbiology and Immunology, University of Missouri, Columbia, Missouri, United States of America aff005;  Structural and Computational Biology Unit, European Molecular Biology Laboratory, Heidelberg, Germany aff006
Vyšlo v časopise: Structures of immature EIAV Gag lattices reveal a conserved role for IP6 in lentivirus assembly. PLoS Pathog 16(1): e32767. doi:10.1371/journal.ppat.1008277
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.ppat.1008277

Souhrn

Retrovirus assembly is driven by the multidomain structural protein Gag. Interactions between the capsid domains (CA) of Gag result in Gag multimerization, leading to an immature virus particle that is formed by a protein lattice based on dimeric, trimeric, and hexameric protein contacts. Among retroviruses the inter- and intra-hexamer contacts differ, especially in the N-terminal sub-domain of CA (CANTD). For HIV-1 the cellular molecule inositol hexakisphosphate (IP6) interacts with and stabilizes the immature hexamer, and is required for production of infectious virus particles. We have used in vitro assembly, cryo-electron tomography and subtomogram averaging, atomistic molecular dynamics simulations and mutational analyses to study the HIV-related lentivirus equine infectious anemia virus (EIAV). In particular, we sought to understand the structural conservation of the immature lentivirus lattice and the role of IP6 in EIAV assembly. Similar to HIV-1, IP6 strongly promoted in vitro assembly of EIAV Gag proteins into virus-like particles (VLPs), which took three morphologically highly distinct forms: narrow tubes, wide tubes, and spheres. Structural characterization of these VLPs to sub-4Å resolution unexpectedly showed that all three morphologies are based on an immature lattice with preserved key structural components, highlighting the structural versatility of CA to form immature assemblies. A direct comparison between EIAV and HIV revealed that both lentiviruses maintain similar immature interfaces, which are established by both conserved and non-conserved residues. In both EIAV and HIV-1, IP6 regulates immature assembly via conserved lysine residues within the CACTD and SP. Lastly, we demonstrate that IP6 stimulates in vitro assembly of immature particles of several other retroviruses in the lentivirus genus, suggesting a conserved role for IP6 in lentiviral assembly.

Klíčová slova:

HIV – HIV-1 – Lentivirus – Lysine – Molecular dynamics – Monomers – Retroviruses – Viral structure


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