A new neuropeptide insect parathyroid hormone iPTH in the red flour beetle Tribolium castaneum

English version

Autoři: Jia Xie ^aff001; Ming Sang ^aff001; Xiaowen Song ^aff001; Sisi Zhang ^aff001; Donghun Kim ^aff002; Jan A. Veenstra ^aff004; Yoonseong Park ^aff002; Bin Li ^aff001
Působiště autorů: Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing, China ^aff001; Department of Entomology, Kansas State University, Manhattan, KS, United States of America ^aff002; Department of Applied Biology, Kyungpook National University, Sangju, Korea ^aff003; INCIA UMR 5287 CNRS, University of Bordeaux, Pessac, France ^aff004
Vyšlo v časopise: A new neuropeptide insect parathyroid hormone iPTH in the red flour beetle Tribolium castaneum. PLoS Genet 16(5): e1008772. doi:10.1371/journal.pgen.1008772
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008772

Souhrn

In the postgenomics era, comparative genomics have advanced the understanding of evolutionary processes of neuropeptidergic signaling systems. The evolutionary origin of many neuropeptidergic signaling systems can be traced date back to early metazoan evolution based on the conserved sequences. Insect parathyroid hormone receptor (iPTHR) was previously described as an ortholog of vertebrate PTHR that has a well-known function in controlling bone remodeling. However, there was no sequence homologous to PTH sequence in insect genomes, leaving the iPTHR as an orphan receptor. Here, we identified the authentic ligand insect PTH (iPTH) for the iPTHR. The taxonomic distribution of iPTHR, which is lacking in Diptera and Lepidoptera, provided a lead for identifying the authentic ligand. We found that a previously described orphan ligand known as PXXXamide (where X is any amino acid) described in the cuttlefish Sepia officinalis has a similar taxonomic distribution pattern as iPTHR. Tests of this peptide, iPTH, in functional reporter assays confirmed the interaction of the ligand-receptor pair. Study of a model beetle, Tribolium castaneum, was used to investigate the function of the iPTH signaling system by RNA interference followed by RNA sequencing and phenotyping. The results suggested that the iPTH system is likely involved in the regulation of cuticle formation that culminates with a phenotype of defects in wing exoskeleton maturation at the time of adult eclosion. Moreover, RNAi of iPTHRs also led to significant reductions in egg numbers and hatching rates after parental RNAi.

Klíčová slova:

Central nervous system – Gene expression – Insects – Parathyroid hormone – RNA interference – Sequence alignment – Vertebrates – chitin

Zdroje

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