IgaA negatively regulates the Rcs Phosphorelay via contact with the RcsD Phosphotransfer Protein
Authors:
Erin A. Wall aff001; Nadim Majdalani aff001; Susan Gottesman aff001
Authors place of work:
National Cancer Institute, Bethesda, Maryland, United States of America
aff001
Published in the journal:
IgaA negatively regulates the Rcs Phosphorelay via contact with the RcsD Phosphotransfer Protein. PLoS Genet 16(7): e32767. doi:10.1371/journal.pgen.1008610
Category:
Research Article
doi:
https://doi.org/10.1371/journal.pgen.1008610
Summary
Two-component systems and phosphorelays play central roles in the ability of bacteria to rapidly respond to changing environments. In E. coli and related enterobacteria, the complex Rcs phosphorelay is a critical player in the bacterial response to antimicrobial peptides, beta-lactam antibiotics, and other disruptions at the cell surface. The Rcs system is unusual in that an inner membrane protein, IgaA, is essential due to its negative regulation of the RcsC/RcsD/RcsB phosphorelay. While it is known that IgaA transduces signals from the outer membrane lipoprotein RcsF, how it interacts with the phosphorelay has remained unknown. Here we performed in vivo interaction assays and genetic dissection of the critical proteins and found that IgaA interacts with the phosphorelay protein RcsD, and that this interaction is necessary for regulation. Interactions between IgaA and RcsD within their respective periplasmic domains of these two proteins anchor repression of signaling. However, the signaling response depends on a second interaction between cytoplasmic loop 1 of IgaA and a truncated Per-Arndt-Sim (PAS-like) domain in RcsD. A single point mutation in the PAS-like domain increased interactions between the two proteins and blocked induction of the phosphorelay. IgaA may regulate RcsC, the histidine kinase that initiates phosphotransfer through the phosphorelay, indirectly, via its contacts with RcsD. Unlike RcsD, and unlike many other histidine kinases, the periplasmic domain of RcsC is dispensable for the response to signals that induce the Rcs phosphorelay system. The multiple contacts between IgaA and RcsD constitute a poised sensing system, preventing potentially toxic over-activation of this phosphorelay while enabling it to rapidly and quantitatively respond to signals.
Keywords:
Point mutation – Phosphates – Protein interactions – Outer membrane proteins – Glucose – Plasmid construction – Cell fusion – Arabinose
Introduction
Bacteria must constantly monitor the integrity of their cell wall and envelope to withstand environmental insults. Osmotic stress, redox stress and envelope disruption demand that the bacterium remodel its exterior to provide protection, often via synthesis and secretion of capsular polysaccharide. Enterobacterales use the Rcs phosphorelay to integrate complex signals from the outer membrane and periplasm, changing gene regulation in response to stress [1, 2]. The Rcs phosphorelay is a complex signal transduction pathway, comprising an outer membrane lipoprotein (RcsF) and three inner membrane proteins (IgaA, RcsC and RcsD); these control the phosphorylation state and thus the activity of the transcriptional regulator (RcsB) (Fig 1A). RcsB in turn regulates production of virulence-associated capsules as well as motility and the expression of other stress-related genes.
Signaling through this pathway is not fully understood. It is known that outer membrane stress generated by cationic polypeptides or cell wall stresses produced by beta-lactams cause a change in the interaction between RcsF and IgaA. IgaA was originally identified in Salmonella and named for intracellular growth attenuation [3]; the E. coli homolog of this gene, yrfF, is referred to here as IgaA. The activated RcsF/IgaA interaction allows the hybrid histidine kinase RcsC to auto-phosphorylate and then pass phosphate to the phosphorelay protein RcsD, a process studied here, which then passes it to response regulator RcsB (Fig 1A). Over-signaling through the phosphorelay leads to cell death, possibly reflecting critical roles of multiple genes within the RcsB regulon. IgaA is essential due to its role as a gating/braking mechanism for the phosphorelay. Deletion of IgaA is only possible in cells that have mutations in rcsC, rcsD, or rcsB [4]. Multiple studies have focused on the interaction of RcsF with IgaA following cell wall stress [5–9], but little has been reported on the downstream action of IgaA [10]. In this work we define RcsD as the direct binding partner of IgaA and define the regions in RcsD that are critical for its interaction with IgaA. Production of RcsD variants that are deficient in IgaA binding cause massive over-signaling through the phosphorelay, resulting in mucoidy and poor viability, similar to phenotypes seen upon loss or inactivation of IgaA itself. Our findings support a model in which IgaA represses the phosphorelay through direct interaction with the phosphotransfer protein RcsD.
Results
A sensitive and flexible assay for the Rcs phosphorelay
We have reinvestigated the Rcs signaling pathway using a newly developed in vivo fluorescent reporter assay for expression of RprA, a small RNA that is a sensitive and specific target of Rcs regulation. RprA levels are nearly undetectable in the absence of RcsB, its direct transcriptional activator, and increase in cells in which the Rcs system is activated [11]. We constructed a transcriptional fusion of the rprA promoter to mCherry, which allows facile and continuous detection of Rcs activation over a wide range. This growth (S1A Fig) and fluorescence assay can be viewed as a function of fluorescence over average cell density, showing a rapid and significant change in slope in cultures treated with an Rcs stimulus (S1B and S1C Fig). Fig 1B shows a bar graph of the fluorescence for cells at OD600 0.4 after wild-type and mutant cells are exposed to polymyxin B nonapeptide (PMBN), a non-toxic small molecule stimulator of Rcs signaling. PMBN stimulus is a useful indicator of pathway status. WT cells are induced in response to PMBN (compare WT bars in Fig 1B); pathway disruptions, by modification or deletion of pathway components, causes dampening or loss of the PMBN response. Cells with rcsB inactivated lose all signal, even the low basal level seen in the absence of PMBN (Fig 1B, S1A, S1B and S1C Fig). The absence of RcsF also lowers overall signal (compare ΔrcsF::cat to WT,—PMBN, Fig 1B). Decreased basal level signaling in the absence of RcsF has been reported before [5, 12–14], but is unambiguous with this assay. Lack of RcsF also greatly dampens the response to PMBN (Fig 1B). It is known that Rcs signaling can be induced in the absence of RcsF, and it is likely that the slight activation by PMBN observed with the ΔrcsF::cat strain occurs by this still unknown mechanism [12, 15]; (Majdalani et al, unpublished).
The hybrid histidine kinase RcsC and the phosphorelay protein RcsD play both positive and negative roles in regulation of RcsB activity. In the absence of RcsC or RcsD the response to PMBN was blocked, but the levels of PrprA-mCherry were significantly higher even in the absence of an inducing signal (Fig 1B). These findings are consistent with earlier work that expression of an PrprA-lac reporter was increased upon deletion of rcsC or rcsD [11, 13]. Cells deleted for rcsC are thought to have lost the ability to de-phosphorylate RcsB that has been phosphorylated from other sources [16–18]. Under our assay conditions, the rcsC deletion strain produced a signal that is 3–4 fold above WT, and the rcsD541 mutant increased PrprA-mCherry expression comparable to that observed with the rcsC deletion. Other rcsD alleles were also examined (see S1D and S1E Fig) and had reasonably consistent behavior.
The increased expression of PrprA-mCherry seen in all rcsD and rcsC strains is likely due in part to phosphorylation of RcsB by the small molecule acetyl phosphate (AcP), combined with the lack of the dephosphorylation activity in the absence of RcsD and RcsC [17, 19]. The influence of AcP is easily seen in an ackA deletion strain that accumulates large intracellular pools of AcP [17] (S1F Fig). In an ackA mutant background, cells wild-type for rcsD and rcsC showed a modest increase in signal (compare black and gray bars), whereas the combination of an ackA mutant with mutations in rcsC or rcsD resulted in high levels of PrprA-mCherry. The increase in signal was fully dependent upon RcsB, as expected (rightmost bar in graph, S1F Fig).
IgaA and RcsD interact directly
We began interrogating how IgaA might interfere with Rcs signaling by examining the interactions of IgaA with downstream members of the phosphorelay, using the bacterial adenylate cyclase two hybrid assay (BACTH). In this assay, synthesis of beta-galactosidase in adenylate cyclase mutant cells is dependent on reconstitution of Bordetella adenylate cyclase from two fragments (T18 and T25, Cya). Each fragment is expressed from a separate vector as a fusion to one of a pair of potentially interacting proteins [20, 21]. The IgaA fusion interacted robustly with the RcsD fusion in two orientations (IgaA-T18/RcsD-T25 and IgaA-T25/RcsD-T18), and expressed beta-galactosidase activity approximately 20-fold greater than either fusion paired with an empty cognate vector, the standard background control (Fig 2A; S2A Fig). This interaction occurred irrespective of the chromosomal presence or absence of other Rcs members (S2B and S2C Fig). However, by the same assay, no significant interaction was detected between IgaA and RcsC (Fig 2A, S2B and S2D Fig). A derivative of RcsC missing the periplasmic region also failed to interact with either RcsD or IgaA (S2E Fig).
Cells expressing IgaA, RcsD, and RcsC fused to Cya fragments produced immunoreactive proteins of the expected size, and the fusion proteins were functional in the Rcs phosphorelay (see Materials and methods, S2F–S2I Fig). This suggests that the RcsC-T25 construct is not completely misfolded. However, we were unable to demonstrate the interaction of RcsC-T18, or of a derivative deleted for the periplasmic region of RcsC with itself or with RcsD (S2D and S2E Fig). Therefore, while we are confident of the interaction of IgaA with RcsD, we cannot interpret the lack of interaction of RcsC with IgaA as demonstrating that these never interact.
Regions in RcsD necessary and sufficient for interaction with IgaA were defined using the bacterial two-hybrid assay (Fig 2B). RcsD1-383, a truncation that includes the trans-membrane and periplasmic regions but is missing most of the cytoplasmic regions including the incomplete Per-Arndt-Sim (PAS-like) domain, resulted in a loss of measurable IgaA interaction. However, IgaA interacted well with RcsD1-461, a C-terminally truncated RcsD that includes the PAS-like cytoplasmic domain (Fig 2B). PAS domains are associated with signal detection in sensor histidine kinases [22]. Somewhat longer RcsD derivatives (RcsD1-683, lacking the ABL and Hpt domains and RcsD1-522, lacking the HATPase, ABL and Hpt domains), varied in their strength of interaction. It seems possible that the RcsD1-522-T25 fusion derivative, with a poorer but measurable interaction, may have a folding defect that interferes with the interaction with IgaA, although the T18 derivative is well expressed (S2G Fig). A fully cytoplasmic RcsD construct (RcsD326-C) that lacked the N-terminal membrane binding regions and periplasmic loop of RcsD did not interact with IgaA, whereas RcsDΔ45–304, which lacks only the periplasmic region, still interacted with IgaA, although at about half the level seen with the WT (Fig 2B). The ability of RcsDΔ45–304 and RcsD1-461 to interact with IgaA suggested that the PAS-like cytoplasmic domain of RcsD, between aa 383 and 461, plays a critical role in the interaction with IgaA. A final construct, RcsD1-461, Δ45–304, was created and tested. This construct, containing the PAS-like domain and localized to the membrane but devoid of the periplasmic loop of RcsD, was able to interact with IgaA as well (Fig 2C). The periplasmic loop of RcsD, while it may contribute to the interaction, is not sufficient to give a signal in the BACTH assay (Fig 2B).
These results are most consistent with RcsD interactions with IgaA within the PAS-like cytoplasmic regions bounded by residue 461. However, this region only gives a robust interaction in the context of the membrane-bound version of RcsD, not when part of a fully soluble RcsD protein (RcsD326-C).
Intriguingly, some of the constructs that displayed significant interactions with IgaA (RcsD1-522, RcsD1-683 and RcsD1-461, but not RcsD1-383 and RcsD326-C) also caused mucoidy in the cloning strain (Stellar E. coli, Clontech; wild-type for all genes of the Rcs phosphorelay), suggesting that overproduction of these constructs causes activation of the phosphorelay. This activating function was further examined, using the PrprA-mCherry reporter as an assay.
Titration of IgaA by overexpression of truncated RcsD
Our BACTH assays suggested that IgaA-dependent repression of Rcs was a consequence of direct interaction between IgaA and RcsD. If so, overproduction of the domains of RcsD capable of this interaction might titrate IgaA away from the chromosomally-encoded RcsD, leading to unregulated signaling through the Rcs phosphorelay. The RcsD fragments studied in the BACTH experiments were cloned without fusion proteins or tags under the control of the arabinose-inducible pBAD promoter. These plasmids were assayed in both rcsD+ and rcsD541 strains containing the PrprA-mCherry reporter fusion to monitor activation of the Rcs phosphorelay. In the absence of arabinose, RcsD is expressed from the pBAD promoter at a level sufficient to complement the rcsD541 mutation, turning down signaling that occurs in strains lacking RcsD (Fig 3A, lower panel; compare RcsD to V). Expression of constructs missing the C-terminal Hpt domain of RcsD (for instance, RcsD1-383 and RcsD1-461) did not inhibit this signaling (Fig 3A, lower panel).
In the rcsD+ host, overproduction of RcsD fragments capable of interacting with IgaA (RcsD1-683, RcsD1-522 and RcsD1-461) resulted in high expression of the PrprA-mCherry fusion, while overproduction of RcsD1-383, which did not interact with IgaA (Fig 2B), also did not stimulate the phosphorelay (Fig 3A, S3A Fig). The requirement for arabinose for the activation of the phosphorelay suggests that the RcsD interacting fragments need to be expressed at high levels to interfere with signaling by IgaA. Significant cellular growth arrest and lysis occurred when RcsD1-683 or RcsD1-522 were overproduced, making quantitative comparisons between the constructs difficult. Nevertheless, the accumulation of PrprA-mCherry fluorescence even at low cell density (before lysis) and the significant activation of signaling were unambiguous (S3A Fig).
The ability of RcsD fragments to activate the phosphorelay in an rcsD+ host correlates well with their ability to interact with IgaA in the BACTH assay, consistent with the model that fragments interact with IgaA and free wild-type RcsD from IgaA repression. These results also reinforce the conclusion from the BACTH assays that a region, between aa 383 and 461, which includes the incomplete PAS-like domain, is required for the titration of IgaA.
Overexpressing full-length RcsD did not induce mucoidy or signaling. Since the intact RcsD can bind and potentially titrate IgaA, this result raised the possibility that one or more of the C-terminal domains of RcsD, absent in the activating/titrating truncated proteins, might exert an inhibitory effect on the phosphorelay. This is consistent with the report that the ABL domain binds RcsB and inhibits its phosphorylation [23].
The RcsD Hpt domain is known to be necessary for transmitting a signal from the RcsC response regulator domain to RcsB [24]. Therefore, we expected that plasmids expressing truncated RcsD constructs that lack the Hpt domain would be completely unable to activate the phosphorelay in rcsD mutants. In the rcsD541 mutant allele background, the basal level of PrprA-mCherry expression is higher than that in a WT host (Figs 1B and 3A, bottom panel, compare V to WT V). A plasmid expressing the intact RcsD reduced expression to levels comparable to the WT strain, consistent with complementation of the rcsD541 mutant (Fig 3A, bottom panel). The activating fragment RcsD1-461 did not induce significant PrprA-mCherry expression in this host, consistent with expectation, since it does not contain the Hpt domain (Fig 3A). Unexpectedly, cells expressing somewhat longer RcsD fragments, truncations RcsD1-522 and RcsD1-683, were able to significantly increase signal when induced in the rcsD541 host, even though both were missing the Hpt domain (S3A Fig, right-hand panel and S3B Fig). This is further discussed in S1 Text.
Signaling by RcsD truncations with reduced capacity to interact with IgaA
Rcs signaling was also assayed in WT and rcsD541 strains carrying plasmids expressing various C-terminal portions of RcsD; all included the Hpt domain. Expression of the ABL-Hpt domains (RcsD686-C), the Hpt domain alone (RcsD792-C), the cytoplasmic portion of RcsD (RcsD326-C), or RcsD deleted for the periplasmic region (RcsD Δ45–304) did not induce signaling in wild type cells (Fig 3B, upper graph). However, each of these plasmids led to significantly increased signaling in the rcsD541 strain, even in the absence of arabinose (Fig 3B, bottom graph). This high-level activity, particularly striking for RcsD326-C and RcsD Δ45–304, is best explained by these proteins sharing two critical properties–availability of an active Hpt domain to pass signal from RcsC to RcsB, and lack of effective repression by IgaA. As expected, the induction activity is dependent upon RcsC (S3D Fig).
RcsD326-C did not interact well with IgaA in the BACTH assay, although it contains the critical PAS-like domain. We suggest this is because it lacks membrane localization (Fig 2B). The shorter RcsD686-C and RcsD792-C were not tested in the BACTH assay. RcsDΔ45–304, however, did interact, albeit at a somewhat reduced level. Cells expressing RcsDΔ45–304 grew poorly in the microtiter plates (6 hour OD600 of 0.12, used in Fig 3B), and colonies containing this plasmid became mucoid in the absence of arabinose induction. Therefore, while IgaA is able to interact with RcsD deleted for the periplasmic region, we suggest that effective repression requires that RcsD include the periplasmic domain.
This was further tested by comparing the behavior of overproduced RcsD1-461, Δ45–304 in a rcsD+ host to the behavior of RcsD, RcsD1-461, and RcsDΔ45–304. Cells containing the pBAD-RcsD plasmids were grown with and without arabinose induction of the pBAD promoter, as well as with and without PMBN (Fig 3C). The proteins were all expressed (S4A Fig). Overexpression of intact RcsD had no effect on Rcs signaling on its own, but inhibited the ability of PMBN to induce signalling, consistent with evidence that the ABL-Hpt domains can inhibit RcsB [23]. Overproduction of RcsD1-461, as in Fig 2A, effectively titrated IgaA; this was also reflected in a significant effect on cell growth (S4B Fig, right panel). Overproduction of RcsDΔ45–304 did not titrate, as noted above, and, presumably because it does have the ABL-Hpt domains, interfered with PMBN induction of the chromosomal copy of RcsD (Fig 3C). RcsD1-461,Δ45–304, expressing the PAS-like domain in the context of a membrane-localized protein, was unable to titrate (no activity with arabinose alone). The comparison of RcsD1-461,Δ45–304 to RcsD1-461 supports a requirement for the periplasmic domain of RcsD for titration. Intriguingly, however, RcsD1-461,Δ45–304 collaborated with PMBN, so that cells showed an increased signal with arabinose and PMBN compared to PMBN alone (Fig 3C). Growth of both RcsDΔ45–304 and RcsD1-461,Δ45–304, but not RcsD or RcsD1-461 was slowed when both PMBN and arabinose were present compared to arabinose alone (right hand panel, S4B Fig, compare cyan and red curves to black and green curves); PMBN had very little effect on growth in the absence of arabinose (left hand panel, S4B Fig).
Selected alleles of the truncated RcsD proteins were introduced into the bacterial chromosome in place of the native rcsD gene and their behavior examined. In these strains, RcsD variants should be expressed from the native promoter, presumably at the physiological level. Alleles were introduced in parallel into either a strain carrying an rcsB deletion, where phosphorelay signaling is off, or into a wild-type host. All tested alleles could be introduced into the rcsB mutant strain, but some alleles were difficult to isolate or were clearly unstable in rcsB+ cells. rcsDΔ45–304 could not be constructed in the rcsB+ host without accumulating secondary loss-of-function mutations elsewhere in rcsD or in rcsB. A mutant that deleted slightly less of the periplasmic domain, rcsDΔ48–304, could be introduced into the chromosome, but the cells were mucoid and constitutively activated for the Rcs phosphorelay (S4C and S4D Fig). The rcsDΔ48–304 allele may be slightly defective for phosphorelay function, and therefore is better tolerated than the rcsDΔ45–304 allele. Cells containing rcsD326-C were also quite mucoid and had significant PMBN-independent signaling; RcsD ABL-Hpt was better tolerated (S4C and S4D Fig). The behavior of these mutants parallels the behavior of the corresponding RcsD plasmids in an rcsD541 strain (Fig 3B). The rcsD truncations, in addition to expressing higher levels of PrprA-mCherry, did not respond to PMBN induction (S4C Fig).
Strains carrying the chromosomal rcsD mutations were tested for their ability to tolerate deletion of igaA. The igaA mutation was introduced into the recipient strain by P1 transduction from a donor (EAW66) containing a bioH::kanR mutation closely linked to igaA::chlR, in a strain containing an unlinked rcsD541 mutation. Kanamycin resistant transductants were isolated and tested for the closely linked chloramphenicol resistance marker in igaA::chlR (S4D Fig). In recipient strains defective for rcsB, rcsC, or rcsD, the linkage of the bioH::kan and igaA::chlR markers was >70%; in a strain WT for the Rcs phosphorelay, linkage was <1%, reflecting the known lethality of igaA mutation in cells with a functional Rcs phosphorelay. Strains carrying the rcsD541 or rcsD841* mutations tolerated loss of igaA well, as expected for strains null for rcsD (S4D Fig). Also as expected, a strain deleted for rcsB (EAW54, S4D Fig) allowed introduction of the igaA::chlR mutation.
The rcsD326-C strain (EAW53), carrying all of the cytoplasmic regions of RcsD, did not tolerate loss of IgaA (S4D Fig). Although chlR colonies were isolated, those colonies had unstable phenotypes; restreaking yielded colonies that were less mucoid or fluorescent, strongly suggesting that cells carrying the igaA deletions could only survive when the Rcs phosphorelay was defective. These results support a model of a critical regulatory contact between IgaA and RcsD in the PAS-like domain. EAW106, expressing RcsD deleted for the periplasmic region (rcsDΔ48–304), also did not tolerate introduction of the igaA deletion (EAW106, S4D Fig).
A strain carrying rcsD686-C (ABL-Hpt), which encodes RcsD lacking all regions involved in IgaA interaction, displayed a lower level of signaling (S4C Fig), was non-mucoid, and, as expected, tolerated the loss of igaA (EAW108, S4D Fig). We would suggest, based on its phenotypes, that this construct is not fully active for passing signal from RcsC, via RcsD, to RcsB.
Our findings, summarized in Fig 3D, allow several conclusions. First, the RcsD periplasmic region is essential for repression by IgaA, but is not sufficient for binding to IgaA by the BACTH assay or for titration of IgaA. Our data are best explained by a direct interaction between the RcsD periplasmic loop and IgaA. The precise role of the trans-membrane (TM) regions flanking the periplasmic loop have not yet been explored. Constructs that lack the periplasmic loop but carry the Hpt domain are capable of induction-independent signaling, presumably because they are at least partially blind to IgaA repression. Second, a critical region of interaction with IgaA lies within the cytoplasmic PAS-like domain of RcsD. This PAS-like domain, in the context of a membrane-bound protein, is sufficient for interaction with IgaA. An RcsD protein carrying both the periplasmic interaction site as well as the PAS-like domain is sufficient to titrate IgaA; neither is sufficient on its own. The combination of these two interaction sites leads to robust repression in the absence of signal. Third, the Hpt domain on its own, or the full cytoplasmic domain, is recessive to RcsD+, and is thus not able to constitutively signal in the presence of functional RcsD.
Critical residues in the cytoplasmic PAS-like domain of RcsD
Alanine scanning mutagenesis of individual conserved residues in the PAS-like domain of RcsD was carried out in the pBAD-RcsD plasmid to look for mutations that affected interaction with IgaA. The pBAD-RcsD mutant plasmids were initially screened based on the fluorescence in an rcsD541 mutant strain grown in the absence of arabinose. In this assay, functional RcsD expressed from the plasmid reduces fluorescence by complementing the rcsD541 allele. A null mutation in the plasmid-borne RcsD copy would not affect fluorescence, and mutant RcsD alleles capable of passing phosphate from RcsC to RcsB that are less sensitive to IgaA repression were expected to have higher fluorescence (see lower panel in Fig 3B, for example). Unexpectedly, many of the plasmids that gave strong signals and were thus thought to be blind to IgaA instead had acquired stop codons within the rcsD open reading frame. These mutants were not further investigated. We instead focused on alanine mutants that retained RcsD function, measured by the ability to complement the rcsD541 mutant, reducing the elevated signal found in rcsD541 to the level found in rcsD+ strains (compare lane 1 and lane 3, S5A Fig). Among the five mutants screened, one was unresponsive to PMBN induction. This mutant, rcsDT411A, was selected for further study because it behaved as though the mutant RcsD was somehow locked in an “off” configuration.
The rcsD T411A mutation was introduced into the chromosome and the strain was tested for its response to PMBN (Fig 4A). The mutant had a lower basal level of Rcs signaling and, as was seen with the plasmid-borne copy, this mutant had a very muted response to PMBN. To determine if the lack of response was unique to PMBN, we tested the response of T411A to A22, an inhibitor of MreB, and to the peptidoglycan inhibitor mecillinam, both of which have also been reported to induce the Rcs System [5, 25]. Both compounds induced our PrprA-mCherry reporter (Fig 4A, WT). As with PMBN, the T411A mutant also failed to respond to A22 or mecillinam (Fig 4A, red bars).
We considered two ways in which T411A might block induction. It might affect the ability of the phosphorelay signal to move from RcsC through the phosphorelay to RcsB, possibly by locking RcsD in the “phosphatase” conformation. Such a defect would be expected to make RcsDT411A insensitive to IgaA. Alternatively, rcsDT411A might be locked off because it cannot be released from IgaA when a stress signal is received. In this case, cells with the rcsDT411A mutation would, like rcsD+, be sensitive to loss of IgaA. This is what we found. The strain carrying a chromosomal rcsDT411A mutation was not able to tolerate deletion of igaA (EAW121, S4D Fig), suggesting that RcsD T411A cannot be activated because of increased or changed interaction with IgaA.
We next asked what regions of IgaA are likely to interact with RcsD and with RcsDT411. Based on our observations with the RcsD truncations (Fig 3D), we predicted that there should be interactions between the periplasmic domains of both proteins, as well as between their cytoplasmic domains via the PAS-like domain of RcsD.
The periplasmic domain of IgaA (shown schematically in Fig 4B) was previously found to interact with RcsF [10]. Here, we found that it is also likely to interact with RcsD, as predicted from our analysis of RcsD domains. In the BACTH assay, deletion of the IgaA periplasmic domain (IgaAΔ384–649) led to loss of the interaction of RcsD and IgaA. Interestingly, RcsD T411A restored some interaction, consistent with our expectation for a contact between the cytoplasmic domains of IgaA and RcsD, increased by the T411A (Fig 4B and S5B Fig). However, deletion of either cytoplasmic loop 1 or cytoplasmic loop 2 of IgaA had essentially no effect on the interaction with wild-type RcsD in the BACTH assay (Fig 4B), suggesting that the primary interactions detected by this assay are between the periplasmic regions.
We also examined the interaction of RcsD with IgaA carrying the periplasmic point mutation L643P, encoding a stable protein (S5C Fig) that caused a partial loss of function mutant in igaA [26]. This mutation led to loss of interaction of IgaA and RcsD (S4D Fig). However, because other alleles at this position (L643A) or nearby mutations in highly conserved residues did not disrupt interaction or activity (S5D and S5E Fig), we conclude that L643 is not likely to directly participate in IgaA function or directly interact with RcsD. The introduction of proline in place of L643 may affect folding or localization of a critical region of IgaA, disrupting its ability to properly interact with RcsD.
Mutations that deleted one of the cytoplasmic loops (Δ36–181, cyt1 or Δ263–330, cyt2) or the periplasmic loop (Δ384–649, peri) of IgaA (see Fig 4B) were introduced in place of the chromosomal igaA gene, in rcsD541 cells carrying the PrprA-mCherry reporter. A mutant with a complete igaA deletion was used for comparison. Introduction of the RcsD plasmid, even in the presence of glucose to maintain low levels of RcsD expression, was poorly tolerated in all the partial igaA deletions as well as the complete deletion. Because secondary mutations arose at a rapid rate (see inset, Fig 4C), assays in liquid were considered untrustworthy, and the phenotypes of the primary transformants were instead evaluated on agar plates (Fig 4C).
Transformation of the RcsD plasmid into cells carrying a deletion of igaA, the igaA periplasmic domain (igaAΔ384–649) or the second cytoplasmic domain (igaAΔ263–330) gave rise to highly mucoid growth, consistent with lack of IgaA function in attenuating Rcs expression. When the RcsD+ plasmid was introduced into cells deleted for the first cytoplasmic domain of IgaA (igaAΔ36–181), streaked colonies were less mucoid, although the PrprA-mCherry reporter was well expressed compared to cells with WT IgaA (Fig 4C left panel). This phenotype was similar when a RcsDT411A plasmid was introduced. Therefore, deletion of cytoplasmic loop one was the best tolerated of the three IgaA partial deletions, but still plays an important role for proper IgaA repression of Rcs signaling. The plasmid expressing RcsDT411A was also not tolerated in strains containing either the full deletion of igaA or the deletion of the IgaA periplasmic domain. However, when the RscDT411A plasmid was put into cells carrying a deletion of the second cytoplasmic domain of IgaA, the colonies had reduced mucoidy and grew more robustly (Fig 4C). These results suggest that RcsD with the T411A mutation has improved interaction with IgaA cytoplasmic loop 1, allowing the mutated IgaA to regain partial function as a brake on RcsD signaling.
Analysis of RcsC domains and involvement in signaling
From previous work, it is clear that RcsC plays an essential role in signaling in the Rcs phosphorelay, as the initiating kinase for the phosphorylation cascade [18]. However, as shown above, full length RcsC and RcsC deleted for the periplasmic domain did not interact with IgaA in the bacterial two hybrid assay (Fig 2 and S2 Fig). The plasmids expressing T18 and T25 fusions to full-length RcsC interfered with cell growth, and the fusion proteins did not interact with RcsD, and thus while our data strongly supports the interaction of IgaA with RcsD, we are cautious in interpreting this negative result with RcsC and IgaA.
Many RcsC constructs in pBAD24 were cytotoxic, causing massive cell lysis without any detectable increase in PrprA-mCherry signal above background, and slow cell growth even in rich defined glucose media, where the pBAD promoter should be only modestly active. To avoid this toxicity, we introduced deletions and substitutions of interest into the chromosomal copy of rcsC and tested the response to induction by PMBN treatment using the PrprA-mCherry reporter. As expected, cells carrying rcsCH479A, mutant in the kinase active site, had low expression and were not responsive to PMBN (Fig 5A). Note that the low activity in this mutant is more like that in the wild-type strain without PMBN than like the deletion of rcsC (Fig 5A), consistent with RcsCH479A retaining phosphatase activity, as reported by Clarke et al [18].
Unexpectedly, cells carrying rcsC Δ48–314, a mutation that deleted the periplasmic portion of RcsC, leaving the TM helices, responded strongly to PMBN, Mecillinam and A22 (Fig 5). PMBN induction in cells carrying rcsC Δ48–314 still required RcsF, strongly suggesting that the signaling pathway is not perturbed (Fig 5A). However, the igaA deletion could be introduced into cells carrying the rcsCΔ48–314 mutation, although they became mucoid and unstable (EAW70, S4D Fig). Strains with a deletion of rcsC or with the rcsCH479A allele were unaffected when igaA was deleted (S4D Fig). We suggest that the rcsCΔ48–314 allele likely has a modestly decreased ability to signal (decreased kinase activity and/or increased phosphatase activity) and/or to pass signal to RcsD, compared to the wild-type protein. Strikingly, our data rule out a role for the RcsC periplasmic loop both in mediating IgaA regulation of the phosphorelay and in signal transduction via RcsF.
Although the periplasmic region is not necessary for RcsC function, it would appear that membrane association is important. Cells carrying a deletion of the membrane spanning portion (RcsC326-C) acted in a similar manner to an rcsC deletion, showing a constitutive level of reporter expression and no response to PMBN (Fig 5A). Consistent with a loss of function for the rcsC326-C allele, the deletion of igaA could be introduced into this strain, and cells remained non-mucoid (EAW56, S4D Fig). While this soluble portion of RcsC did interact with RcsD, it did not interact with IgaA (S6A Fig). A series of periplasmic deletions with different linker lengths all responded to PMBN to some extent (S6B Fig). Finally, a chimeric construct in which the MalF TM and periplasmic region replaced the rcsC periplasmic region also restored the ability of the cell to respond to PMBN (S6B Fig), albeit with a higher basal level of signaling in the absence of PMBN.
Discussion
The results reported here provide a new view of how IgaA transduces inducing signals within the complex Rcs phosphorelay (Fig 1A, Fig 6). IgaA, a multipass membrane protein, is a strong negative regulator of Rcs. As previously described, inducers such as PMBN, believed to disrupt LPS interactions, A22, an inhibitor of MreB, and mecillinam, a beta-lactam antibiotic affecting peptidoglycan, act by changing the nature of the RcsF/IgaA interaction. This leads to a change, presumably a decrease, in IgaA’s interaction with components of the downstream phosphorelay. We find that IgaA has critical interactions with the phosphotransfer protein RcsD. We were unable to detect interactions of IgaA with the RcsC histidine kinase. While this failure to detect a RcsC-IgaA interaction may reflect limits in the BACTH assays used, we also find that the TM and periplasmic portions of RcsC are not required for sensing and transducing the PMBN inducing signal (Fig 5, S6B Fig). In our model, the change in the IgaA-RcsD interaction allows RcsC-generated phosphate to flow from RcsC to RcsD, and from there to RcsB, activating RcsB-dependent transcription. Deletion and mutation analysis of RcsD identified multiple regions important for IgaA-dependent regulation, separate from the Hpt domain needed for phosphate flow from RcsC to RcsD and from RcsD to RcsB. These observations help to explain why RcsD includes not only an Hpt domain but also trans-membrane and signaling domains. We suggest that the use of RcsD as the direct target of IgaA has allowed the development of a poised signaling complex, without impinging on structures in RcsC necessary for histidine kinase activity. In addition, this branched signaling pathway may leave RcsC able to respond to other regulatory signals.
Multipartite interactions of RcsD and IgaA regulate signaling: anchors and switches
Our analysis of the regions of RcsD and IgaA necessary for interaction and regulation suggests at least two points of contact between these proteins, each with different roles in regulation. A primary strong contact is proposed to be between the periplasmic domain of RcsD and the periplasmic domain of IgaA. The TM and periplasmic domains of RcsD (amino acids 1–383) are necessary but not sufficient for repression by IgaA; the periplasmic region of IgaA (aa 384–649) is similarly essential for IgaA function and drives the interaction of IgaA and RcsD in BACTH assays (Figs 2B and 4B). It seems likely that these periplasmic domains directly contact each other (Fig 6). Previous work by others has demonstrated that overproduction of the cytoplasmic loops of IgaA can support repression of the phosphorelay and allow depletion (but not deletion) of wild-type IgaA [10]. Presumably under these conditions, the loss of the periplasmic contacts is overcome by high levels of portions of IgaA mediating other repressive contacts. That leaves the question of whether the periplasmic contact we detect is part of the switch–is it responding to changes in signaling when all proteins are at their usual physiological levels? Certainly in some sense the periplasmic region of IgaA must be involved in signal transduction, since this is where RcsF talks to IgaA [5, 6, 10]. Whether RcsF and RcsD directly compete for binding to IgaA remains to be investigated.
However, our finding that a mutation in the cytoplasmic PAS-like domain of RcsD is sufficient to block signaling suggests that this critical interaction in the cytoplasmic domains serves as at least part of the switch. The cytoplasmic PAS-like domain of RcsD is necessary for regulation and for interaction with IgaA, measured both by BACTH assay and by titration of IgaA (Figs 2B and 3A). It seems likely that this cytoplasmic domain of RcsD contacts one or both cytoplasmic loops of IgaA. Our data favors a critical contact of the PAS-like domain of RcsD with the cytoplasmic loop 1 of IgaA (Fig 6). Both cytoplasmic loop 1 and cytoplasmic loop 2 of IgaA were necessary for RcsD to function properly (Fig 4C) in agreement with previous work [10]. We suggest that the interaction of cytoplasmic loop 1 and RcsD, in the region around T411, constitutes the regulatory switch for this system. Deletion of loop 1 is the least detrimental in terms of bacterial growth and signaling (Fig 4C), suggesting that the contacts outside cytoplasmic loop 1 are sufficient for enough IgaA repression of RcsD to support viability. Our model suggests that the additional repressive interaction in loop 1 is normally lost upon Rcs stimulus (in the presence of PMBN, for instance), and that the anchor contacts in the periplasm and possibly with IgaA loop 2 ensure that signaling is never so high that the cell dies. In the T411A mutant, this stimulus-sensitive contact becomes stronger, so that the system becomes uninducible (Fig 4A). This can be seen in the bacterial two-hybrid assay as some restored interaction in the absence of the IgaA periplasmic region (Fig 4B) and a decrease in mucoidy for the cytoplasmic loop 2 deletion (Fig 4C). We do not currently have any direct evidence that cytoplasmic loop 2 is contacting RcsD, but certainly deletion of this loop, like deletion of the periplasmic region, abrogates repression. This defect is partially suppressed by improving the interaction of IgaA with RcsD, via the T411A mutation (Fig 4C), strongly suggesting that T411 can operate in the absence of cytoplasmic loop 2.
In work by Collet and coworkers, overproduction of cytoplasmic loop 1 [10] was, by itself, capable of partially repressing an Rcs reporter in a strain depleted of IgaA; repression was to a similar extent to that seen with both cytoplasmic regions, supporting the critical role we suggest here for the RcsD PAS-like domain contacts with IgaA cytoplasmic loop 1, highlighted by behavior of the T411A mutation.
RcsD is an unusual phosphorelay protein
Phosphate flow in complex phosphorelays such as Rcs is from His (kinase domain) to Asp (response regulator domain of RcsC) to His (RcsD phosphotransfer protein) to Asp (RcsB response regulator domain). RcsD is a large inner membrane protein with many additional domains; its domain organization suggests that duplication of an ancestral protein may have given rise to RcsC and RcsD. Our results suggest critical roles for these additional regions of RcsD.
Consistent with RcsD’s role as an anchor for IgaA, alignments suggest significant regions of conservation within the periplasmic domain of RcsD, apparently more so than the similarly sized RcsC periplasmic domain, which we show here is not critical for signaling (Fig 5A, S7 Fig). There is significant conservation as well in the truncated PAS domain, but less conservation in the inactive HATPase domain than in the active parallel RcsC domains (S7B Fig). Future work will be necessary to identify the periplasmic interaction points of RcsD with IgaA and to understand whether the RcsD HATPase domain plays any critical role in regulation.
Alternative signaling pathways remain to be understood
The complexity of the Rcs phosphorelay provides opportunities for signals to regulate RcsB activity independently of the RcsF-IgaA-RcsD interaction network. Some transcription factors interact directly with RcsB, independent of its phosphorylation, to make heterodimers that regulate specific sets of genes (reviewed in [1]). In addition, there is evidence for activation of RcsB-dependent genes, dependent upon RcsC and RcsD, but independent of RcsF. For instance, overproduction of the DjlA DnaJ-like chaperone or mutation in dsbA, the gene encoding the periplasmic disulfide bond formation protein DsbA, both lead to RcsF-independent induction of the Rcs phosphorelay, possibly suggesting that alterations in protein folding may be the inducing event [1, 18, 27].
One other unexplored aspect of our work is the possible expression of low levels of the C-terminal domains of RcsD, to produce a short phosphotransfer protein that would not be subject to IgaA regulation. This is discussed in S1 Text, and remains to be explored in the future.
Overall, while a critical step in the best understood signaling pathway is clarified here, there is still much to learn about Rcs, other modes of signaling to the phosphorelay, and exactly how the IgaA/RcsD interactions modulate phosphate movement from RcsC through RcsD to RcsB. We do not yet know if RcsD acts as a monomer or a dimer, either with itself or RcsC, and whether its quartenary structure changes during signaling. Given the range of genes regulated by RcsB, and the importance of these genes for bacterial behavior, the complexity and potential flexibility in sensing and signal transduction in this system may not be surprising.
Materials and methods
Bacterial growth conditions and strain construction
Cells were grown in LB with appropriate antibiotics (ampicillin 100μg/ml, kanamycin 30–50μg/ml, chloramphenicol 10μg/ml for cat-sacB allele, usually listed here as cat or 25 μg/ml for chlR alleles, tetracycline 25μg/ml, gentamicin 10μg/ml, zeocin 50μg/ml); 1% glucose was added in some cases to reduce basal level expression of PBAD and PLac promoters. For fluorescence/growth assays, strains were grown in MOPS minimal glucose or minimal glycerol (Teknova). Strains were constructed via recombineering and/or P1 transduction with selectable markers, as outlined in S1 Table. Strains, plasmids, oligonucleotides and gBlocks used in this study are listed in S1, S2 and S3 Tables. Oligonucleotides and gBlocks were from IDT DNA, Coralville, IA.
For recombineering, cells carrying the chromosomal mini-λ Red system or the plasmid-borne Red system (pSIM27) were grown in LB, without or with Tetracycline respectively, at 32° C to an OD600 of ~0.4–06. At mid-log, cultures were transferred to a water bath at 42° C to induce expression of the λ-Red system for 15 minutes and then immediately chilled in an ice-water slurry for 10 minutes prior to washing in sterile ice-cold water to make electrocompetent cells. 100 ng of ss oligo DNA or dsDNA (PCR product or gBlock) were used in the electroporation; 1 ml of LB or SOC was added for recovery before plating on selective plates [28]. Truncations and point mutations other than rcsD543, described in the strain table, were introduced in place of chromosomal copies of genes, leaving no marker or scar, by first deleting the gene of interest and inserting in its place the counter-selectable ara-kan-kid cassette from CAI_91. The cassette was then replaced with the desired allele, provided either as a PCR product or a gBlock. This cassette, a gift of C. Ranquet (BGene Genetics, Grenoble), expresses the Kid toxin under the control of an arabinose-inducible promoter. Cells carrying the ara-kan-kid counter-selectable marker cassette were grown with added 1% glucose in the media to repress. Counter-selection for removal of the ara-kan-kid cassette was done on LB-1% arabinose plates. All plasmid and chromosomal mutations were confirmed by sequencing using flanking primers.
Because many of the plasmids used here showed poor transformation using TSS, plasmids were generally introduced into cells using CaCl2 transformation [21].
DNA and strain manipulation and mutagenesis
Polymerase chain reactions were performed using Pfu Ultra II polymerase (Agilent) or Clontech Hifi polymerase (Takara). Primers used in this study are listed in S3 Table. PCR products were purified using column purification (Qiagen) according to the manufacturer’s instructions. Gibson assemblies were performed using the Clontech In-Fusion HD Cloning Kit (Takara) and transformed into either Clontech Stellar Cells, NEB DH5alpha F’lacIQ or NEB Turbo cells containing LacIq.
Alanine-scanning mutagenesis was carried out by SGI-DNA (San Diego, CA) using their BioXP system on pBAD24-RcsD (pEAW11). We ordered single mutants targeted at conserved residues within the cytoplasmic region of RcsD, from residue 326–683. The products from the company were first transformed into Stellar E. coli (Clontech), extracted and retransformed into EAW19, screening for fluorescence on minimal glucose-ampicillin agar plates, in comparison to a pRcsD+ plasmid and the empty vector. Out of 35 mutants screened, ten had fluorescence levels comparable to the pRcsD+ control and five of them were further studied (S5A Fig). Another 17 had higher fluorescence than either the pBAD vector or the pRcsD+ control, but sequencing these isolates showed them to have picked up random mutations (stop codons) in addition to the designed ones and were not further studied. However, we note that the high levels of fluorescence for these isolates is best explained by assuming that they expressed, likely at a low level, the Hpt phosphotransfer domain, downstream from the observed stop codons.
Bacterial Adenylate Cyclase Two-Hybrid Assay
In the bacterial adenylate cyclase two hybrid assay (BACTH), an adenylate cyclase mutant strain is used to assay for beta-galactosidase activity engendered when the T18 and T25 portions of adenylate cyclase are reconstituted, allowing cAMP/CRP to activate the lac operon. On their own, T18 and T25 will not form adenylate cyclase efficiently unless they are fused to two interacting proteins [21]. Tags were C-terminal to avoid interference with protein insertion into the membrane. Primary data for the experiments in the Figures can be found in S1 Data.
Complementation assays were used to test the RcsD and RcsC fusion proteins for function. Plasmids expressing RcsD-T25 and RcsC-T25 were introduced into strains containing deletions for the appropriate gene; after transformation, the cells were transduced with P1 grown on strain NM357, containing igaA::chlR, selecting for chloramphenicol resistance. In a strain deleted for rcsD or rcsC, the igaA deletion can be introduced by P1 transduction. However, the fusion plasmids blocked the ability of cells to be transduced with igaA::chlR, consistent with them complementing their respective deletions (S2F Fig).
igaA co-transduction frequencies
bioH/igaA co-transduction frequencies were used to determine which strains could support loss of IgaA. bioH, at 3544844 nt, is linked to igaA (position 3526469). The bioH::kanR mutant from the Keio collection [29] was introduced by P1 transduction into an rcsD541 igaA::chlR mutant (EAW17), selecting for kanamycin resistance and retention of chloramphenicol resistance (igaA::chlR), to create the donor strain EAW66 (rcsD541 bioH::kanR igaA::chlR). Because rcsD is inactive in this strain, it can tolerate loss of igaA. P1 transduction from this donor to recipient strains was carried out, selecting for Kanamycin Resistance and then screening 50–100 colonies for linkage to igaA::chlR. In rcsB, rcsC or rcsD null recipients, the co-transduction frequency was 78%. In a wild-type strain, the linkage dropped to zero, consistent with the known lethality of an igaA deletion [4, 5] (S4D Fig).
Fluorescence assays
Fluorescence assays for Rcs activation were performed in 96 well plates in a Tecan Spark 10m or a Tecan Spark spectrophotometer. Strains carried a transcriptional fusion of mCherry, at the ara locus, to the promoter for sRNA RprA, as a reporter for Rcs pathway activation, referred to here as PrprA-mCherry. Fluorescence of cells was measured in MOPS glucose minimal media (Teknova) unless otherwise stated. The pBAD24 plasmid was used for overexpression of RcsD fragments in strains expressing araE constitutively to ensure homogenous arabinose uptake [30]. For cells expressing proteins from pBAD, overnight cultures in MOPS minimal 0.2% glucose were washed with MOPS minimal glycerol to eliminate residual glucose, then diluted into fresh MOPS minimal glycerol media (.05% glucose, 0.5% glycerol) with 0.02% arabinose or 0.2% glucose as an uninduced control. Polymyxin B nonapeptide (PMBN; Sigma), a non-toxic polymyxin derivative, was used at 20 or 40 ug/ml, as indicated in figure legends, to induce the Rcs system. To check for Rcs induction by other known compounds, A22, an MreB inhibitor was used at 5ug/ml, and mecillinam was used at 0.3ug/ml.
Each combination of a strain and condition was performed in technical triplicate in the microtiter plate, with biological replicates performed on different days. Optical density and mCherry fluorescence were monitored every fifteen minutes for seven hours (S1A, S1B and S1C Fig). At the end of six hours, measurements of fluorescence at equivalent OD600 values (0.4 +/- 0.03 after starting at OD600 .03-.05) were converted to bar graphs of fold change of fluorescence with respect to the wild type strain. Some strains arrested growth early and never achieved 0.4 OD600, and the OD600 at 6 hours for those are noted on the graphs. Six hours marks the time when the wild type strain begins to transition to stationary phase, and ODs become less interpretable due to cell aggregation in the well bottom. Primary data for all Figures can be found in S1 Data.
Western blots
For the chemiluminescent western blots in S2I and S5C Figs, plasmids transformed into NEB Turbo cells (NEB, Ipswich, MA) were used. Colonies were inoculated into LB media containing Amp (100 μg/ml) and grown for 3–4 hours at 37 C. At that point, IPTG was added to a final concentration of 1mM and cultures allowed to grow further. At 30, 60, and 120 min post-induction, a 1 ml aliquot was taken and TCA precipitated, washed in acetone and resuspended in sample buffer standardized to OD. 10 μl volumes were loaded and run on 4–12% NuPage gradient gels (Invitrogen, CA). Transfer was done in an iBlot2 onto nitrocellulose membranes as per manufacturer’s specifications (Invitrogen, CA). After blocking in 5% milk, membranes were incubated in CyaA T18 mouse monoclonal primary antibody at 1:5000 (Santa Cruz Biotechnology, CA) overnight at 4 C, washed and incubated with an anti-mouse AP-conjugated secondary antibody (1:10,000) (Cell Signaling, MA) for 2 hrs. Development was with Novex CDP Star with Nitro Block II (Invitrogen, CA) according to manufacturer’s instructions and exposed on a BioRad ChemiDoc MP imager (BioRad, CA).
For fluorescent western blots (S1E, S2G, S2H, S2J and S4A Figs), strains were grown in LB media to mid-log OD~ 0.4. A 1 ml aliquot was precipitated and washed in TCA-acetone as above. Primary rabbit polyclonal RcsD or RcsB antibodies were used at 1:5,000 (Covance, PA) while the loading control primary antibody was a mouse monoclonal anti-Ef-Tu at 1:10,000 (Hycult Biotech, PA). Secondary fluorescent antibodies anti-rabbit Starbright700 and anti-mouse DyLight800 were used at 1:5000 and 1:10,000 respectively (BioRad, CA). Imaging was done on a ChemiDoc MP.
Supporting information
S1 Fig [rfu]
Measurement of Rcs activity by a fluorescent assay (relevant to ).
S2 Fig [eaw1]
Interaction of IgaA and RcsD in a Bacterial Two-Hybrid Assay (relevant to ).
S3 Fig [eaw8]
Analysis of RcsD function in signaling.
S4 Fig [eaw53]
Effects of RcsD Truncations on the ability to tolerate loss of IgaA.
S5 Fig [peaw8]
Mutation in RcsD cytoplasmic region blocks signaling.
S6 Fig [peaw2]
The RcsC periplasmic loop is dispensable for signaling.
S7 Fig [tif]
: Conservation in RcsC and RcsD.
S1 Data [zip]
These files contain the primary data used to generate graphs presented in the Figures and Supplementary Figures.
S1 Text [docx]
A possible role for the RcsD Hpt domain in the unexpected activation of the phosphorelay with certain RcsD mutant proteins.
S1 Table [xlsx]
Strains used. Strain name, relevant genotype and method of construction or reference, if previously published, are shown.
S2 Table [xlsx]
Plasmids used.
S3 Table [xlsx]
Primers and gBlocks used.
Zdroje
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Článek vyšel v časopise
PLOS Genetics
2020 Číslo 7
- Duševní onemocnění se u teenagerů přenáší v rámci školní třídy
- Prof. Libor Grubhoffer: Nová varianta mpox je příležitostí pokročit v poznání původce varioly
- Co obsahují nové pokyny WHO pro dozor nad antibiotickou rezistencí?
- Naděje pro diabetiky: transplantace beta-buněk jim jednou nahradí inzulin
- Ukažte mi, jak kašlete, a já vám řeknu, co vám je
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- Repression of tick microRNA-133 induces organic anion transporting polypeptide expression critical for Anaplasma phagocytophilum survival in the vector and transmission to the vertebrate host
- Brassinosteroids regulate root meristem development by mediating BIN2-UPB1 module in Arabidopsis
- Genetic and metabolomic architecture of variation in diet restriction-mediated lifespan extension in Drosophila