#PAGE_PARAMS# #ADS_HEAD_SCRIPTS# #MICRODATA#

Glucose transporter 10 modulates adipogenesis via an ascorbic acid-mediated pathway to protect mice against diet-induced metabolic dysregulation


Authors: Chung-Lin Jiang aff001;  Wei-Ping Jen aff001;  Chang-Yu Tsao aff001;  Li-Ching Chang aff002;  Chien-Hsiun Chen aff002;  Yi-Ching Lee aff001
Authors place of work: Institute of Cellular and Organismic Biology, Academia Sinica, Taipei, Taiwan aff001;  Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan aff002
Published in the journal: Glucose transporter 10 modulates adipogenesis via an ascorbic acid-mediated pathway to protect mice against diet-induced metabolic dysregulation. PLoS Genet 16(5): e32767. doi:10.1371/journal.pgen.1008823
Category: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008823

Summary

The development of type 2 diabetes mellitus (T2DM) depends on interactions between genetic and environmental factors, and a better understanding of gene-diet interactions in T2DM will be useful for disease prediction and prevention. Ascorbic acid has been proposed to reduce the risk of T2DM. However, the links between ascorbic acid and metabolic consequences are not fully understood. Here, we report that glucose transporter 10 (GLUT10) maintains intracellular levels of ascorbic acid to promote adipogenesis, white adipose tissue (WAT) development and protect mice from high-fat diet (HFD)-induced metabolic dysregulation. We found genetic polymorphisms in SLC2A10 locus are suggestively associated with a T2DM intermediate phenotype in non-diabetic Han Taiwanese. Additionally, mice carrying an orthologous human Glut10G128E variant (Glut10G128E mice) with compromised GLUT10 function have reduced adipogenesis, reduced WAT development and increased susceptibility to HFD-induced metabolic dysregulation. We further demonstrate that GLUT10 is highly expressed in preadipocytes, where it regulates intracellular ascorbic acid levels and adipogenesis. In this context, GLUT10 increases ascorbic acid-dependent DNA demethylation and the expression of key adipogenic genes, Cebpa and Pparg. Together, our data show GLUT10 regulates adipogenesis via ascorbic acid-dependent DNA demethylation to benefit proper WAT development and protect mice against HFD-induced metabolic dysregulation. Our findings suggest that SLC2A10 may be an important HFD-associated susceptibility locus for T2DM.

Keywords:

Gene expression – Cell differentiation – Adipocytes – Adiponectin – Adipokines – Vitamin C

Introduction

Large-scale genome-wide association studies (GWAS) have successfully identified many genes that contribute to type 2 diabetes mellitus (T2DM); however, those genes explain only a small proportion of the disease susceptibility and heritability [1, 2]. Since transitions in dietary pattern have greatly contributed to the increased prevalence of obesity and accelerated the spread of T2DM epidemic worldwide, it is clear that environmental factors heavily affect the risk of T2DM. Therefore, the gene-diet interactions may be crucial to accelerate development of T2DM. Additionally, rare variants in specific genes, which cannot easily be identified via GWAS, might have strong effects on T2DM risk [35].

Ascorbic acid has been implied to reduce risk of T2DM [6]. Epidemiological studies have shown that plasma ascorbic acid levels are inversely correlated with waist-to-hip ratio [69], which reflects the accumulation of visceral fat and is highly associated with the risk of T2DM [10]. Furthermore, animal studies demonstrated that ascorbic acid supplementation reduces inflammation and adiposity in high-fat-diet (HFD)-fed animals [6, 11]. However, clinical trials testing ascorbic acid supplementation for the prevention or treatment of obesity and its co-morbidities have produced inconsistent results [6, 12]. Thus, the possible mechanisms and physiological effects that link ascorbic acid with reduced risk of T2DM require further exploration.

Ascorbic acid is an antioxidant, and it is also an enzyme co-factor contributing in a broad range of biological processes [13]. Ascorbic acid and its oxidized form, dehydroascorbic acid (DHA), can be transported into cells and intracellular compartments by active sodium ascorbic acid transporters (SVCTs) and facilitative glucose transporter members (GLUTs), respectively [14]. Loss-of-function mutations in GLUT10 lead to a rare autosomal-recessive connective tissue disorder called arterial tortuosity syndrome (ATS; OMIM 208050) [15]. We and others have demonstrated that GLUT10 is a critical factor in the maintenance of intracellular ascorbic acid levels and redox hemostasis in vitro and in vivo [1620]. GLUT10 is localized to the endomembrane system, mitochondria and the nuclear envelope, and it mediates intracellular DHA transport in aortic smooth muscle cells (ASMCs), adipocytes and fibroblasts [1416, 19]. The DHA transported into subcellular compartments can be regenerated into ascorbic acid, which enhances cellular uptake of DHA as a mechanism to maintain intracellular ascorbic acid levels and redox homeostasis [1621]. Therefore, GLUT10 may play a critical role in ascorbic acid homeostasis under stress conditions [16, 20]. Ascorbic acid is essential for hydroxylation of prolyl and lysyl residues during collagen synthesis and assembly. The effects of GLUT10 on ascorbic acid-dependent collagen synthesis/assembly and redox balance might explain connective tissues abnormalities observed in mice with functional GLUT10 deficiency and ATS patients [15, 17, 20].

Interestingly, the chromosomal region around GLUT10 the gene (SLC2A10) has been associated with T2DM in French and Finnish sib-pairs though linkage analysis [22, 23]. However, a direct association between the genetic polymorphisms in SLC2A10 locus and T2DM has not been found with GWAS [2429]. The reasons for this discrepancy are unknown but may be related to the physiological role of GLUT10. Notably, high expression of GLUT10 was detected in white adipose tissue (WAT), especially in stromal vascular fraction (SVF) cells in both humans and mice [16, 30]. Adipose tissue serves as a major energy reserve, and it is an active endocrine organ secreting various adipokines to regulate whole-body energy homeostasis. Thus, either an excess or a deficiency of adipose tissue may have harmful metabolic consequences [31]. Based on these known GLUT10 functions, the expression pattern, and its potential association with T2DM, we suspected that GLUT10 might have roles in metabolism and contribute to T2DM.

Here, we used population studies, a mouse model carrying a GLUT10 rare variant (Glut10G128E mice) and in vitro adipogenesis experiments to test this idea. We demonstrate that genetic variants in SLC2A10 locus are associated with T2DM-related intermediate traits in human subjects. Furthermore, Glut10G128E mice have reduced adipogenesis and WAT development and are susceptible to HFD-induced metabolic dysregulation. Finally, we demonstrate GLUT10 modulates ascorbic acid-mediated DNA-demethylation and gene expression of Cebpa and Pparg to affect adipogenesis. Together our results demonstrate that GLUT10 positively regulates adipogenesis, WAT development and has beneficial effects on metabolism. Our findings also suggest that SLC2A10 may be an important susceptibility locus for HFD-induced T2DM.

Results

SLC2A10 is suggestively associated with T2DM intermediary traits

We first examined whether genetic polymorphisms of SLC2A10 might be associated with T2DM intermediate traits that reflect an increased risk for the disease, as a direct association between SLC2A10 genetic polymorphisms and T2DM has not been found with GWAS. By analyzing the GWAS Central database (https://www.gwascentral.org) [32], we found that several genetic polymorphisms in SLC2A10 locus are modestly associated with multiple T2DM-related phenotypes, including fasting plasma glucose, fasting insulin and body mass index in non-diabetic populations of independent human populations and studies (S1 Table). To further validate these results, we examined the association of SLC2A10 genetic polymorphisms with glycated hemoglobin level (HbA1c), a well-known risk factor for developing T2DM [33] in non-diabetic population of Taiwan Super Control Study [34, 35]. To increase the power and better detect genetic effects, we included the imputed single nucleotide polymorphisms (SNPs) [34]. Using this approach, we were able to identify genetic polymorphisms in SLC2A10 were suggestively associated with HbA1c level (the SNP with the best association had P-value = 1.11 × 10−5) (Table 1).

Tab. 1. HbA1c-associated variants in the SLC2A10 locus among Han Taiwanese.
HbA1c-associated variants in the <i>SLC2A10</i> locus among Han Taiwanese.

Glut10G128E mice are susceptible to HFD-induced glucose intolerance and insulin resistance

To directly test whether GLUT10 may play a role in T2DM, we examined whether metabolism might be affected in mice carry a GLUT10 variant with known impacts on cellular physiology. The Glut10G128E mouse strain was generated by N-ethyl-N-nitrosourea (ENU) mutagenesis [36], and the same human rare variant (rs1226198239) was identified and predicted to be benign (S2 Table, heighted in yellow). The variant encodes a protein with an altered residue in extracellular loop 5 that is conserved in mammals (S1A and S1B Fig). GLUT10G128E protein has impaired mitochondrial targeting, decreased DHA uptake, and decreased intracellular ascorbic acid levels in ASMCs [16, 20]. We backcrossed the Glut10G128E mice from their original C3H background onto the C57BL/6J background that is widely used in metabolic disease research. Glut10G128E mice on a C57BL/6J had similar phenotypes to those previously observed on the C3H background. Glut10G128E mice have a normal appearance and do not exhibit signs or pathological features of ATS-related major artery phenotypes at birth. The mice develop observable pathology in vascular wall of major arteries at 10 months of age [20, 36]. We therefore used the Glut10G128E mice as a model to study how an SLC2A10 polymorphism might affect metabolism in mice.

We compared metabolism-related parameters in Glut10G128E and WT mice on a normal chow diet with ad libitum feeding (CD) at 20 weeks of age. Glut10G128E mice had no significant differences from WT in body weight gain or fasting glucose levels (Fig 1A, 1B and 1C). Interestingly, Glut10G128E mice did exhibit significantly higher HbA1c levels (Fig 1D), similar to the findings in our human population study. Despite this increased level of HbA1c, Glut10G128E mice maintained normal fasting insulin levels (Fig 1E) and showed no difference from WT in their ability to clear a glucose bolus or insulin sensitivity, which were respectively measured by the glucose tolerance test (GTT) and insulin resistance test (ITT) (Fig 1F and 1G). Aging is a major risk factor for metabolic dysregulation, so we further examined mice on CD at 24 months of age. Glut10G128E mice exhibited fasting blood glucose levels and GTT results similar to WT mice (Fig 1H). Collectively, our data showed that while Glut10G128E mice have higher HbA1c levels, no obvious metabolic abnormalities are present when the mice are fed with CD.

Fig. 1. Glut10G128E mice are predisposed to HFD-induced glucose intolerance and insulin resistance.
<i>Glut10</i><sup><i>G128E</i></sup> mice are predisposed to HFD-induced glucose intolerance and insulin resistance.
Glut10G128E mice and WT mice were fed with a CD or HFD from 5 to 20 weeks of age. Data were collected from the mice at indicated of age or at the conclusion of feeding (20 weeks of age). (A) Representative photograph of mice at 20 weeks of age. Each square on the green mat is 1 cm × 1 cm. (B) Body weights are shown for indicated ages. n = 20−40 mice per group. (C) Fasting glucose, (D) HbA1c levels, and (E) fasting insulin levels were measured at 20 weeks of age. (F) Glucose tolerance test (GTT) was performed in 16-week-old mice and (G) insulin tolerance test (ITT) was performed in 18-week-old animals. Right panels in F and G respectively show the areas under the GTT and ITT curves (AUC). n = 6 mice per group. (H) GTT was performed in WT and Glut10G128E mice on a CD at 24 months of age. n = 6 mice per group. The data are shown as mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.

We then examined the metabolic consequences of feeding Glut10G128E mice and WT with a HFD from 5 weeks of age to 20 weeks of age. Surprisingly, on a HFD, Glut10G128E mice gained more weight than WT mice (Fig 1B), despite both genotypes exhibiting comparable food intake, physical activity, energy expenditure, and respiratory exchange ratio (RER) on either a CD or HFD (S2 Fig). However, trends toward reductions in physical activity, VO2, VCO2 and heat production were observed in Glut10G128E mice on a HFD (S2B–S2F Fig). On the HFD, Glut10G128E mice had significantly higher fasting blood glucose, HbA1c levels, and fasting insulin levels compared to WT mice (Fig 1C, 1D and 1E). Furthermore, Glut10G128E mice exhibited worse glucose tolerance and insulin sensitivity than WT mice (Fig 1F and 1G). We then examined insulin sensitivity in insulin-responsive organs in HFD-fed WT and Glut10G128E mice by probing insulin-induced AKT phosphorylation, a downstream target of the insulin receptor. After insulin injection, phosphorylated AKT was highly elevated in epididymal WAT (eWAT), liver, and skeletal muscle of HFD-fed WT mice (S3A–S3C Fig). In contrast, the insulin induction of AKT phosphorylation was attenuated in eWAT of Glut10G128E mice on HFD (S3A Fig). Of note, AKT phosphorylation in eWAT of Glut10G128E mice on CD was also lower than WT controls (S3A Fig). In summary, our data showed that Glut10G128E mice are predisposed to HFD-induced weight gain, glucose intolerance, and insulin resistance.

Glut10G128E mice are susceptible to HFD-induced inflammation and fibrosis in eWAT, adipokine dysregulation, and ectopic lipid deposition

We next asked how the Glut10G128E variant might predispose mice to HFD-induced metabolism dysregulation. We compared the gross morphologies of T2DM-related tissues and organs in Glut10G128E and WT mice on a CD. Notably, Glut10G128E mice showed reduced size and weight of visceral epididymal WAT (eWAT) on CD (Fig 2A and 2B). In contrast, the morphology, mass, and histology of subcutaneous inguinal WAT (sWAT), interscapular brown adipose tissue (iBAT), liver, and pancreas of Glut10G128E mice all appeared normal on CD (Fig 2A and 2B; S4A–S4D Fig). Histology analysis of eWATs revealed a reduced average adipocyte cell size in Glut10G128E eWATs that was mainly attributable to an increase in the proportion of small adipocytes (Fig 2C, 2D and 2E). Accordingly, Glut10G128E mice exhibited low levels of lipid storage in eWAT (Fig 2F) and the body fat composition (Fig 2G), while body lean composition was not different from WT controls (Fig 2H). Thus, the most apparent phenotypes in CD-fed Glut10G128E mice were observed in eWAT.

Fig. 2. Glut10G128E mice are susceptible to HFD-induced inflammation and fibrosis in eWAT as well as ectopic lipid deposition.
<i>Glut10</i><sup><i>G128E</i></sup> mice are susceptible to HFD-induced inflammation and fibrosis in eWAT as well as ectopic lipid deposition.
Glut10G128E mice and WT mice were fed a CD or HFD from 5 to 20 weeks of age. Data were collected from mice at 20 weeks of age. (A) Representative photographs of eWAT and liver from experimental mice. Each square on the green mat is 1 cm × 1cm. (B) Tissue weights. (C) Hematoxylin and eosin (H&E) staining, trichrome staining, and CD68 immunohistochemistry of eWAT sections. (D) The cross-sectional areas of adipose cells in eWAT are presented as the log2 ratio of cells in a particular size range in Glut10G128E mice relative to that in WT mice on a CD or HFD. (E) The average adipocyte size in eWAT. D and E, more than 1000 adipocytes were measured from the eWAT sample from each mouse. n = 6 mice per group. (F) Triglyceride (TG) content in eWAT. (G and H) Body fat and body lean compositions. (I-L) The adiponectin, leptin, IL-6, and TNFα levels in serum. (M) Total cholesterol (TCHO) levels and (N) free fatty acid (FFA) levels in serum. (O) H&E staining of liver sections. (P) Triglyceride (TG) content in liver. Data are shown as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.

The eWAT in Glut10G128E mice was then compared with that in WT mice on a HFD. Glut10G128E mice had slightly lower mass of eWAT (Fig 2A and 2B) but no significant difference from controls in lipid storage on a HFD (Fig 2F). Histological analyses showed that eWAT from HFD-fed Glut10G128E mice had high heterogeneity in its cell size distribution and no significant difference from WT in average adipocyte size (Fig 2C, 2D and 2E). The appearance crown-like structures were frequently observed in on HFD-fed Glut10G128E eWAT (Fig 2C). The structures indicating macrophage accumulation is a hallmark of adipose tissue inflammation and contributes to tissue fibrosis. Macrophage infiltration and fibrosis were confirmed by CD68 staining, a protein highly expressed in macrophages, and Masson’s trichrome staining for collagen. The results revealed increased macrophage infiltration and extracellular matrix deposition in Glut10G128E eWAT (Fig 2C). Moreover, mRNA expression of macrophage marker, EGF-like module-containing mucin-like hormone receptor-like 1 (EMR1; also known as F4/80), and fibrosis-promoting factor, transforming growth factor β (TGF-β), were markedly increased in eWAT of HFD-fed Glut10G128E mice (S5A Fig). We conclude that Glut10G128E mice are susceptible to HFD-induced eWAT inflammation and fibrosis.

WAT secretes biologically active adipokines, such as adiponectin, leptin, tumor necrosis factor-α (TNF-α), and interleukin-6 (IL-6), which control systemic energy homeostasis and are often affected by pathophysiological conditions [37]. The serum level of adiponectin was reduced, while serum levels of leptin, IL-6 and TNF-α were higher in HFD-fed Glut10G128E mice (Fig 2I–2L). Interestingly, the serum levels of adiponectin and IL-6 were also altered in CD-fed Glut10G128E mice (Fig 2I and 2K). The mRNA expression levels for these adipokines in Glut10G128E eWAT were consistent with the serum levels on both CD and HFD (S5A and S5B Fig). Thus, Glut10G128E mice have altered expression of adipokines on CD, and the adipokine dysregulation is further augmented on HFD.

Inflammation and fibrosis in eWAT and adipokine dysregulation can contribute to ectopic lipid deposition in other tissues [3840]. As such, we compared ectopic lipid deposition in Glut10G128E mice and WT mice on a HFD. The serum levels of total cholesterol and free fatty acids were significantly increased in Glut10G128E mice (Fig 2M and 2N). Ectopic lipid accumulation was observed in the liver of HFD-fed Glut10G128E mice, as demonstrated by the increased size, weight and triglyceride content of the liver, along with an increase in liver fat vacuoles (Fig 2A, 2B, 2O and 2P). Ectopic lipid accumulation was also observed in the iBAT of Glut10G128E mice, as increased occurrence of fat vacuoles was observed (S4B Fig). The size and mass of sWAT were also increased in Glut10G128E mice (S4A Fig and Fig 2B). However, no obvious histological changes were observed in sWAT of Glut10G128E mice (S4C Fig). Overall, hyperlipidemia and ectopic lipid deposition were presented in HFD-fed Glut10G128E mice.

The pancreas appeared to be unaffected in Glut10G128E mice on either a CD or HFD, according to insulin secretion, tissue morphology and mass, and histology of pancreatic islets (Fig 1E, Fig 2B and S4A and S4D Fig). Furthermore, no obvious vascular abnormalities were observed in major arteries or adipose tissues of Glut10G128E mice on a CD or HFD, based on histology and functional architecture of the adipose vasculature revealed by fluorescent microbead perfusion (S6 Fig).

Together, Glut10G128E mice exhibit reduced eWAT and irregular adipokine expression on CD; the mice are further predisposed to HFD-induced eWAT inflammation and fibrosis, augmented adipokine dysregulation, hyperlipidemia and ectopic lipid accumulation.

Glut10G128 mice have reduced eWAT and altered adipokine profiles at an early age

Because Glut10G128E mice exhibit significantly reduced mass and cell size of eWAT coincident with irregular adipokine levels on CD, which are critical pathophysiological mechanism underlying HFD-induced metabolic dysregulation. We therefore compared eWAT and adipokine expression in Glut10G128E mice and WT mice at an early age. In mice, WAT compartments begin to develop during the late gestational stages and then exhibit a marked postnatal expansion just before 10 weeks of age [41]. GLUT10 expression was detected at high levels in eWAT of juvenile mice (3 weeks) compared with adult mice on CD (20 weeks) (Fig 3A). Interestingly, Glut10G128E mice exhibited compromised eWAT development at 3 weeks of age, as indicated by reduced mass, reduced triglyceride content, and reduced average adipocyte size with an increase small adipocyte population (Fig 3B–3F). Consistent with reduced eWAT in Glut10G128E mice, serum levels of most adipokines were reduced, while IL-6 levels were increased, according to the results of an adipokine array (Fig 3G and 3H). The changes in adiponectin and IL-6 expression were validated in serum by ELISA and mRNA expression in eWAT by RT-PCR (Fig 3I and 3J). These results demonstrated that Glut10G128E mice have reduced eWAT and altered adipokine profile at an age as early as 3 weeks.

Fig. 3. Glut10G128 mice have compromised eWAT development and altered adipokine profiles at an early age.
<i>Glut10</i><sup><i>G128</i></sup> mice have compromised eWAT development and altered adipokine profiles at an early age.
(A) GLUT10 mRNA expression in eWAT was analyzed by RT-PCR from Glut10G128E mice and WT mice on a CD at 3 weeks of age (3W) or 20 weeks (20W) of age. n = 6 mice per group. (B-L) Glut10G128E and WT mice were analyzed at 3 weeks of age. (B-F) Glut10G128E mice have compromised eWAT development. (B) Tissue weight and (C) the triglyceride (TG) content of eWAT. n = 6 mice per group. (D) Hematoxylin and eosin (H&E) and Pref-1 immunohistochemistry staining of eWAT sections. (E) The cross-sectional area of adipose cells in eWAT is presented as the log2 ratio of the area of cells in a particular size range in Glut10G128E mice relative to that of the same size range in WT mice. (F) The average adipocyte size in eWAT. E and F, n = 6 mice per group; more than 1000 adipocytes were analyzed in each mouse. (G -J) Glut10G128E mice have altered adipokine profiles. (G) The adipokine levels in serum of Glut10G128E and WT mice were determined by a mouse adipokine protein array. Serum samples from 8 mice per group were pooled. The arrowheads indicate signals with observable changes. (H) Quantification of relative levels of adipokines with observable changes. The average expression from two independent experiments and duplicate data in each array were analyzed separately. (I) Adipokine levels in serum were analyzed by ELISA. n = 4 mice per group. (J) mRNA expression of adipokines in eWAT was analyzed by RT-PCR. n = 6 mice per group. (K-L) Glut10G128E mice have reduced adipogenesis in eWAT. (K) The mRNA expression of adipogenesis-related genes in eWAT was determined by RT-PCR. n = 6 mice per group. (L) Protein expression levels were analyzed by western blotting. Protein samples from 6 mice per group were pooled. Data are shown as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.

GLUT10 is required for adipocyte differentiation in vivo

We next explored the potential mechanism through which GLUT10 regulates eWAT development. We performed RNA sequencing (RNA-seq) analysis of eWAT from 3-week-old WT and Glut10G128E mice to profile the genes affected by the GLUT10 variant. Ingenuity pathway analysis (IPA) revealed that the GLUT10 variant affected genes involved in adipogenesis, WAT development, adipokines expression and energy metabolism (S7 Fig). We also noticed an increase in non-adipocyte cell population in Glut10G128E eWAT (Fig 3D). Furthermore, GLUT10 expression is higher in cells from the stromal vascular fraction of eWAT from both humans and mice [30], and its expression is readily detected in adipocyte precursor cells, including mouse embryonic fibroblasts (MEFs) and 3T3-L1 cells; of note, GLUT10 expression was not detected in immune cells (S8 Fig). We speculated that adipogenesis in Glut10G128E eWAT might be somehow impaired, so we examined adipogenesis in eWAT of WT and Glut10G128E mice at 3 weeks. Remarkably, immunohistochemical staining of preadipocyte factor 1 (Pref-1), a preadipocyte marker, was markedly increased in Glut10G128E eWAT (Fig 3D). Consistent with the increase in Pref-1 staining in Glut10G128E eWAT, both mRNA expression and protein levels of Pref-1were increased as well (Fig 3K and 3L); meanwhile, the expression levels of adipocyte-specific fatty acid binding protein 4 (FABP4) and key adipogenic transcription factors, including peroxisome proliferator-activated receptor gamma (PPARγ), PPARγ coactivator 1-alpha (PGC-1α) and CCAAT/enhancer-binding protein (C/EBPα), were all reduced in Glut10G128E eWAT (Fig 3K and 3L). These results suggest that adipogenic differentiation is impaired in Glut10G128E eWAT at an early age.

GLUT10 deficiency cell-autonomously impairs adipogenesis and alters adipokine expression

To directly examine whether GLUT10 regulates adipogenesis, we used two in vitro adipogenic systems, MEFs and 3T3-L1 cells. MEFs were isolated from Glut10G128E and WT mice, while 3T3-L1 cells with stable knockdown of Glut10 expression (shGlut10) or stable expression of shLuc (as a control) were used to test whether GLUT10 deficiency produces similar effects to the variant. As expected, adipogenic differentiation was much less successful in both Glut10G128E MEFs and shGlut10 3T3-L1 cells, as evidenced by lower levels of lipid accumulation and triglyceride content (Fig 4A–4F). Consistent with its apparent role in adipogenesis, GLUT10 expression was high in preadipocytes and reduced after induction of adipogenic differentiation in 3T3L1 cells (Fig 4G). The expression levels of key adipogenic transcription factors (C/EBPα and PPARγ), adipocyte marker (FABP4), and a major adipokine (adiponectin), were all significantly reduced in shGlut10 3T3-L1 cells during adipocyte differentiation (Fig 4H–4K). In contrast, the expression of inflammatory adipokines (IL-6 and TNF-α), was induced in shGlut10 3T3-L1 cells (Fig 4L and 4M). These in vitro results agree well with our in vivo data (Figs 2 and 3), showing that GLUT10 regulates adipogenic differentiation and expression of adipokines.

Fig. 4. GLUT10 deficiency cell-autonomously impairs adipogenesis and alters adipokine expression.
GLUT10 deficiency cell-autonomously impairs adipogenesis and alters adipokine expression.
(A-C) MEFs were isolated from Glut10G128E and WT mice and induced adipogenic differentiation for 7 days. (A) Representative images show oil red O staining. (B) Quantification of oil red O staining and (C) intracellular triglyceride content (TG) are shown. (D-F) Glut10-knockdown (shGlut10) and control (shLuc) 3T3-L1 cells were induced for adipogenic differentiation for 6 days. (D) Representative images show oil red O staining. (E) Quantification of oil red O staining and (F) intracellular triglyceride (TG) content respectively. (G-M) The relative mRNA expression levels of Glut10 and indicated genes were determined by RT-PCR after induction of adipogenic differentiation for the indicated number of days. n = 3 independent experiments per group. Data are shown as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.

GLUT10 regulates intracellular ascorbic acid levels in preadipocytes and promotes adipogenesis

Next, we examined if the reduced adipogenic differentiation in GLUT10-deficient preadipocytes is due to low intracellular ascorbic acid levels. Ascorbic acid could be transported into cells and intracellular compartment in its reduced form or oxidized form by SVCTs or GLUTs respectively. GLUT10 was expressed at relatively higher levels in 3T3-L1 preadipocytes compared to other expressed transporters (S9A Fig). Knockdown of GLUT10 expression did not alter the expression of other transporters (S9B Fig). As expected, DHA uptake and intracellular ascorbic acid levels were reduced in shGlut10 3T3-L1 cells cultured in 75 μM DHA (Fig 5A). Cultured the cells in a physiological concentration of ascorbic acid (75 μM) where ascorbic acid could be oxidized to DHA, the intracellular and nuclear ascorbic acid levels were also reduced in shGlut10 3T3-L1 cells (Fig 5B). These results demonstrate that GLUT10 maintains intracellular ascorbic acid levels in 3T3-L1 preadipocytes under physiological conditions. Supplementation with 75 μM ascorbic acid improved adipogenic differentiation in 3T3-L1 cells (Fig 5C and 5D). Furthermore, ascorbic acid supplementation had more pronounced effects in shGlut10 3T3-L1 cells (2-fold increase in lipid content) than shLuc 3T3-L1 cells (1.3-fold increase in lipid content) (Fig 5C and 5D). The same results were also validated in Glut10G128E MEFs (S10 Fig). Consistently, ascorbic acid supplementation induced the expression of key adipogenic genes, Cebpa and Pparg, in shGlut10 3T3-L1 cells (Fig 5E and 5F). These results suggest that GLUT10 is required for maintaining intracellular ascorbic acid level, and ascorbic acid positively regulates expression of Cebpa and Pparg and adipogenesis.

Fig. 5. GLUT10 modulates intracellular ascorbic acid levels and regulates adipogenesis.
GLUT10 modulates intracellular ascorbic acid levels and regulates adipogenesis.
(A) 3T3-L1 preadipocytes were incubated with 75 μM 14C-labeled DHA for 30 min. Intracellular 14C DHA levels (left panel) and intracellular ascorbic acid (AA) levels (right panel) were determined. (B) 3T3-L1 preadipocytes were cultured in medium containing 75 μM AA, and intracellular (left panel), cytoplasmic and nuclear AA levels (right panel) were determined. (C-F) shGlut10 and shLuc 3T3-L1 cells were cultured in normal medium (4 μM AA from serum, mock) or supplemented with 75 μM AA for 2 days. Adipogenic differentiation was induced for 6 days in C and D. Adipogenic differentiation was induced for the indicated number of days in E and F. (C) Representative images of oil red O staining and (D) quantification of the oil red O staining. (E and F) Relative mRNA expression of Cebpa and Pparg was determined by RT-PCR. n = 3 independent experiments per group. Data are shown as the mean ± SEM. *P < 0.05, **P < 0.01, ***P < 0.001.

GLUT10 maintains 5-hydroxymethylcytosine (5hmC) levels and gene expression of Cebpa and Pparg

We further explored the mechanism by which GLUT10 regulates ascorbic acid-mediated expression of Cebpa and Pparg and adipogenesis. Recent studies have shown that ascorbic acid serves as an enzyme cofactor for ten-eleven translocation (TET) dioxygenases, which catalyze 5-methylcytosine (5mC) demethylation to 5hmC and regulate cellular reprogramming and differentiation [4246]. We therefore examined if GLUT10 affects ascorbic acid-dependent DNA demethylation. The shGlut10 3T3-L1 cells had lower global 5hmC levels than shLuc 3T3-L1 cells, and ascorbic acid supplementation increased global 5hmC levels (Fig 6A). We then directly investigated the DNA demethylation status in gene regions that are known to control expression of the central adipogenesis-regulating genes, Cebpa and Pparg [47, 48]. Importantly, ascorbic acid supplementation increased 5hmC levels in Cebpa and Pparg in 3T3-L1 cells (Fig 6B and 6C). These increased 5hmC levels in Cebpa and Pparg were positively correlated with the gene expression levels (Fig 5E and 5F), demonstrating that decreased intracellular ascorbic acid levels in shGlut10 3T3-L1 cells reduces DNA demethylation in Cebpa and Pparg and affects genes expression.

Fig. 6. GLUT10 modulates ascorbic acid-mediated DNA demethylation of Cebpa and Pparg and adipogenesis.
GLUT10 modulates ascorbic acid-mediated DNA demethylation of <i>Cebpa</i> and <i>Pparg</i> and adipogenesis.
(A-C) shGlut10 and shLuc 3T3-L1 cells were cultured in standard culture medium (4 μM AA from serum) or supplemented with 75 μM AA (AA). (A) Global 5hmC levels were quantified with an ELISA-based assay. (B and C) 5hmC levels in Cebpa and Pparg were quantified by 5hmC immunoprecipitation coupled with qPCR. (D) Schematic diagram depicting the inhibition of ascorbic acid (AA)-mediated TET DNA demethylation by DFO, DMOG or NPA. (E and F) shGlut10 and shLuc 3T3-L1 cells were treated with either DFO (10 μM), DMOG (100 μM), NPA (50 μM), or a vehicle control along with 75 μM AA for 2 days and adipogenic differentiation was induced for 6 days. (E) Representative images of oil red O staining and (F) quantification of the oil red O staining. Data are shown as the mean ± SEM. n = 3 independent experiments per group. *P < 0.05, **P < 0.01, ***P < 0.001.

We further inhibited the ascorbic acid/iron/α-KG-dependent TET reaction to determine whether TET activity is necessary for proper adipogenesis. Cells were treated with the iron chelator desferrioxamine (DFO), the α-KG analog dimethyloxalylglycine (DMOG), or the chemical inhibitor 3-nitropropinic acid (NPA) to prevent succinate dehydrogenase A (SDHA) conversion of succinate to fumarate to inhibit TET enzyme activity (Fig 6D). DFO and DMOG both dose-dependently inhibited adipogenesis of shGlut10 3T3L1 cell (S11A–S11F Fig). Furthermore, treatment of cells with low doses of DMOG, DFO or NPA, which did not affect adipogenesis in control cells, was sufficient to significantly reduce adipogenesis in shGlut10 3T3-L1 cells (Fig 6E and 6F; S11 Fig). These results reveal that shGlut10 3T3-L1 cells were highly sensitive to DFO, DMOG or NPA-mediated inhibition of adipogenesis. These findings suggest that positive regulation of ascorbic acid by GLUT10 promotes TET enzyme activity to facilitate adipogenesis.

Based on the combined evidence from our in vitro models, we conclude that GLUT10 modulation of intracellular and nuclear ascorbic acid levels affects TET-mediated DNA demethylation in Cebpa and Pparg genes, which regulates expression of the genes and adipogenesis (Fig 7). In Glut10G128E mice, the reduced adipogenesis contributes to reduced eWAT development, altered adipokine profile, and predisposal to HFD-induced inflammation and fibrosis of eWAT and severe metabolic consequences (Fig 7).

Fig. 7. Model depicts how GLUT10 modulates ascorbic acid levels to protect mice against HFD-induced metabolic dysregulation.
Model depicts how GLUT10 modulates ascorbic acid levels to protect mice against HFD-induced metabolic dysregulation.
GLUT10 modulates ascorbic acid-mediated DNA demethylation and gene expression of Pparg and Cebpa to regulate adipogenesis, thereby affecting WAT development and metabolism.

Discussion

In this study, we demonstrate that GLUT10 plays an important role in WAT development and its loss of function contributes to HFD-induced metabolic dysregulation, providing new insights into the long-standing question of whether SLC2A10 gene is associated with T2DM. Furthermore, our study provides an example of a genetic factor that affects WAT development to increase susceptibility toward diet-triggered T2DM in mice. We expect these findings will stimulate sophisticated population studies to probe gene-diet interactions in the development of T2DM, while also contributing key knowledge about disease prediction and prevention.

Large-scale GWAS have successfully uncovered more than 100 common variants associated with T2DM. However, these common variants explain only a small proportion of T2DM disease susceptibility and heritability, suggesting that rare variants and environmental interactions also contribute to T2DM risk [49]. Recent candidate gene approaches combined with functional studies have successfully identified rare variants with strong effects on T2DM risk [35]. Here, we identify a suggestive association between the SLC2A10 locus and HbA1c levels in a non-diabetic Han Taiwanese population. While more than 700 rare variants (allele frequency < 1%) in SLC2A10 gene are predicted to affect GLUT10 function (S2 Table and S3 Table), only a few have been demonstrated to cause ATS (S2 Table, labeled in red), and most variants are predicted to be benign or of uncertain significance. A large-scale study will therefore be needed to test the association of these rare variants with disease. Nevertheless, it is useful to perform experimental characterization of the functional effects of the potential variants. Here, we demonstrated that mice carrying the G128E variant have increased HbA1c levels, and they are predisposed to develop HFD-induced T2DM. We further demonstrated that adipogenesis is not only affected in Glut10G128E eWAT and Glut10G128E MEFs, but it is also affected in GLUT10 knockdown 3T3-L1 cells, suggesting other variants with compromised GLUT10 expression or function might have similar effects. Finally, although the contribution of rare variants to overall disease burden in a population may be modest, the relatively large individual effect sizes may be useful for clinical risk prediction and disease prevention for certain individuals.

After SLC2A10 was identified as the gene that causes ATS, patients with mild phenotypes were verified by sequencing the gene [5052]. The life expectancy of ATS patients with mild phenotypes is longer than it is for those with severe phenotypes, and the connective tissue manifestations become more prominent with age [53]. Although no association with overt diabetes was observed in ATS patients [50], obesity was reported in some older ATS patients [54, 55], and one report mentioned that a carrier father developed hyperinsulinemia after rapid weight gain (10 kg in 2 years) [50]. Our findings suggest that follow-up studies monitoring blood glucose levels and diet control might be of benefit for ATS patients and carriers.

Glut10G128E mice have reduced adipogenesis, reduced WAT development, decreased adiponectin and increased IL-6 in eWAT at an early age. Reduced adipose tissue development (lipodystrophy) can lead to almost all the features of metabolic syndrome [56], and a subset of lipodystrophy patients exhibit reduced plasma adiponectin and numerous metabolic complications [57]. Adiponectin is predominantly secreted by adipocytes and plays various protective roles in the body, including anti-inflammatory, antidiabetic, antiatherogenic, and cardioprotective effects [58]. The shGlut10 3T3-L1 cells have reduced adipogenesis and reduced adiponectin levels. Previous reportes showed that serum levels of adiponectin are negatively correlated with visceral fat [59], and several lines of evidence showed that pro-inflammatory cytokines (i.e., TNF-α and IL-6) reduce adiponectin expression [60]. This reduced adiponectin level was observed prior to the onset of obesity and insulin resistance [60]. Thus, the reduced adiponectin in Glut10G128E mice might be due to reduced WAT and increased pro-inflammatory cytokines in Glut10G128E eWAT. Moreover, the dysregulation of adipokines in Glut10G128E mice might contribute to HFD-induced metabolic dysregulation. Notably, low-grade inflammation, especially increased IL-6, has been suggested to be an important factor to enhance dysregulation of fatty acid metabolism, adipocyte hypertrophy, immune cell infiltration, and the overproduction of extracellular matrix; by these actions, caloric excess promotes inflammation and fibrosis in eWAT [6163]. The expression of lipogenesis and lipolysis genes was disturbed in eWAT of Glut10G128E mice on either a CD or HFD (S12A Fig), indicating a disturbance in lipid metabolism in Glut10G128E eWAT. This dysregulation of fatty acid metabolism may explain why the adipocyte size in Glut10G128E eWAT was reduced on CD and how it was increased to a level comparable to WT on HFD. Although sWAT was increased in Glut10G128E mice on HFD, no histological differences with WT were observed, suggesting the metabolic dysregulation in HFD-fed Glut10G128E mice might be mainly attributed to eWAT. To this point, significant differences in the functional effects of subcutaneous (sWAT) and visceral fat (eWAT) on metabolic dysfunction have been widely observed [64]. Together, these results and previous findings further support the idea that reduced adipogenesis, and adipokine dysregulation in eWAT of Glut10G128E mice predispose the mice to HFD-induced metabolic dysregulation.

In this work, we demonstrated GLUT10 modulates intracellular/nuclear ascorbic acid levels in 3T3L1 cells. Notably, decreased intracellular ascorbic acid levels was also osbserved in MEFs isolated from Glut10G128E compared to WT controls (S10A Fig). Additionally, intracellular ascorbic acid levels were lower in freshly isolated SVF cells from Glut10G128E mice compared with SVF cells from WT mice (S13A Fig). Mice can endogenously synthesize ascorbic acid in the liver, and serum ascorbic acid levels were significantly increased in Glut10G128E mice on HFD (S13B Fig), suggesting an attempt at compensation for disrupted ascorbic acid homeostasis in Glut10G128E mice. The mechanisms contributing to this increased serum ascorbic acid levels in Glut10G128E mice are as yet unknown, however the observation supports the idea that GLUT10 deficiency disturbs ascorbic acid homeostasis in vivo.

We showed that GLUT10 modulates ascorbic acid-dependent DNA demethylation to regulate the expression of C/EBPα and PPARγ genes for adipogenic differentiation in 3T3L1 cells. Emerging evidence shows that ascorbic acid induces TET-dependent DNA demethylation to fine-tune gene expression and regulate cell reprograming [42, 43, 65]. Interestingly, ascorbic acid has been shown to enhance adipogenic differentiation by stimulating expression of C/EBPα and PPARγ at the initiation of adipogenesis though uncharacterized mechanisms [66]. However, the mechanisms governing intracellular/nuclear ascorbic acid levels and how might ascorbic acid might stimulate expression of C/EBPα and PPARγ are incompletely described. Our study provides evidence to support these previous findings and bridge some gaps in knowledge. Furthermore, C/EBPα and PPARγ are key transcription factors that orchestrate adipogenic and mitochondrial function [67]. Mitochondrial dysfunction decreases adipogenesis and increases expression of proinflammatory adipokines, such as IL-6, reducing the metabolic flexibility of WAT [6870]. Indeed, the oxygen consumption rate (OCR), which reflects mitochondrial respiration, was significantly reduced in shGlut10 3T3L1 cells (S14A Fig). Ascorbic acid supplementation significantly improved OCR, while suppressing the increased expression of IL-6 and TNF-α in shGlut10 3T3L1 cells (S14B Fig). These results agree with previous studies and suggest that decreased C/EBPα and PPARγ expression and decreased mitochondrial function might increase the expression of IL-6 and TNF-α in shGlut10 3T3L1 cells. In addition, ascorbic acid is involved in diverse iron/α-KG dioxygenase reactions [13], which might also contribute to adipogenesis. Finally, in addition to aortic smooth muscle cells and WAT, GLUT10 expression can be detected in stomach and intestine in mice (S8 Fig). At present, we cannot exclude the possibility that GLUT10 deficiency in other tissues might affect adipogenesis through alternative mechanisms.

It is well established that ascorbic acid is an antioxidant/free radical scavenger. Hence, we explored the possibility that the antioxidant function of ascorbic acid may be important in adipogenesis. Consistent with the decreased intracellular ascorbic acid level, intracellular and mitochondrial reactive oxygen species (ROS) levels were increased in shGlut10 3T3-L1 cells (S15A and S15B Fig). Pre-incubating shGlut10 3T3-L1 cells with ascorbic acid or other antioxidants, NAC (N-acetyl-cysteine) and MitoQ (mitochondria-targeted coenzyme Q10), reduced intracellular and mitochondrial ROS levels (S15A and S15B Fig). Importantly, however, only ascorbic acid could enhance adipogenesis in shGlut10 3T3-L1 cells (S15C and S15D Fig). Furthermore, the intracellular and mitochondrial ROS levels were relatively low in shLuc 3T3-L1 cells, and while ascorbic acid supplementation did not further reduce the intracellular or mitochondrial ROS levels in these cells, it did promote adipogenesis (S15C and S15D Fig). These results suggest that ascorbic acid promotion of adipogenesis in 3T3L1 cells might not require its function as a free radical scavenger function. However, whether the disturbance of ascorbic acid homeostasis in Glut10G128E mice might affect oxidative stress and inflammation to contribute to the eWAT phenotypes and metabolic consequences should be further explored.

In summary, we report previously uncharacterized roles of GLUT10 in adipogenesis and WAT development, which affect the sensitivity to HFD-induced metabolic dysregulation in mice. We also demonstrated that the SLC2A10 gene is associated with intermediate traits of T2DM in a non-diabetic population. Thus, our study provides an example where a gene involved in WAT development affects sensitivity to HFD induced metabolic dysregulation.

Materials and methods

Ethics statement

The study was approved by the institutional review board and the ethics committee of Academia Sinica (AS-IRB01-101120), and informed consent was obtained from all participants in accordance with institutional requirements and the Declaration of Helsinki Principles.

Association of SLC2A10 polymorphisms with HbA1c level

We tested the association of the SLC2A10 polymorphism with HbA1c in the Taiwan Super Control Study (TWSC) [35]. Subjects with diabetes were excluded, as defined by physician diagnosis, medication use, or HbA1c ≥ 6.5%. A total of 920 participants were included. The demographics of the participants were: age, 50 ± 17.8 (mean ± SD); 50% male; BMI, 23.6 ± 3.5 (mean ± SD); HbA11c, 5.2 ± 0.4 (mean ± SD). For HbA1c measurement, whole blood was collected from the participants, and HbA1c was analyzed by Cation Exchange HPLC TOSOH HIC-723 G7 (TOSH CORPRATION, Tokyo, Japan) and presented as the National Glycohemoglobin Standardization Program (NGSP) percent. SNPs were genotyped using Illumina Hap550duo chip with call rate ≥ 95%. The variants were imputed using post-QC genotypes and HapMap CHB+JPT (release no.22) as a reference panel. SNPs were analyzed for HbA1c association fitted to a linear regression model adjusted for sex and BMI.

Mice

Glut10G128E mice on a C3HeB/FeJ background [36] were backcrossed to C57BL/6J for more than 10 generations, and animals on C57BL/6J background were used in this study. Mice were housed in a controlled environment with a 14-h light/10-h dark cycle at 21–23°C under specific pathogen-free conditions. Standard rodent diet (CD) contained 13% energy from fat (LabDiet 5010 rodent Diet, PMI Nutrition International Inc., Brentwood, MO, USA), and the high-fat diet (HFD) contained 60% energy from fat (58Y1, Young Li Trading Co., New Taipei City, Taiwan). All animal protocols were approved by the Institutional Animal Care and Utilization Committee, Academia Sinica (Protocol #14-12-795). Male mice were used in this study. No data were excluded in the analyses.

Glucose and insulin tolerance test (GTT and ITT)

For fasting blood glucose measurement, blood samples were collected from mice after overnight fasting. For the GTT, mice at 16 weeks of age were fasted for 18 h and then given an intraperitoneal injection of glucose (2 g/kg). For the ITT, mice at 18 weeks of age were fasted for 8 h and administered an intraperitoneal injection of insulin (0.75 U/kg, Humulin R U100, Lilly, Eli and Company, Indianapolis, IN, USA). Blood samples were collected 15, 30, 45, 60, 75, and 90 min post-injection. Blood glucose levels were assessed using a glucometer (Accu-Chek Performa, Roche Medical Diagnostic Equipment Co., Taiwan).

Body composition

Mouse body composition was analyzed by Bruker’s minispec LF50 Body Composition Analyzers in the Taiwan Mouse Clinic at Academia Sinica.

Histological analysis and immunohistochemistry

Tissue sections were stained with hematoxylin and eosin (H&E) or Masson’s trichrome stain. For immunohistochemistry, deparaffinized tissue sections were incubated with primary and secondary antibodies, as indicated in Supplementary Data.

Triglyceride content

Adipocytes or minced tissues were lysed in lysis buffer (5% NP-40 in H2O). The lysis solutions were slowly heated to 80–100°C for 2–5 min to ensure triglycerides were dissolved. Total triglyceride content was determined with a Triglyceride Quantification Colorimetric/Fluorometric kit (BioVision Inc, Milpitas, CA, USA).

Blood chemistry and adipokine assays

Blood was collected from cardiac puncture for blood chemistry and adipokine assays.

The enzyme activities of GOT and GPT, in addition to TCHO and TG levels were analyzed from serum samples using Fuji biochemical slides and a Fuji Dri-Chem 4000i analyzer (Fujifilm Cooperation, Taipei, Taiwan) in the Taiwan Mouse Clinic at Academia Sinica. Plasma levels of adiponectin, leptin, IL-6, and insulin were measured using mouse ELISA kits (Merck Millipore, Taipei, Taiwan). The plasma free fatty acid was measured using an ELISA kit (ab65341, Abcam, Cambridge, MA, USA), and blood HbA1c was measured using the mouse Hemoglobin A1c (HbA1c) Assay kit (Crystal Chem, Elk Grove Village, IL, USA).

Quantitative reverse transcriptase polymerase chain reaction (qRT-PCR)

Total RNA from cells or tissues was isolated, converted to cDNA, and then subjected to qRT-PCR as described previously [20]. Expression data were normalized to 36B4 mRNA in adipose tissues or β-actin mRNA levels in liver tissues. Gene-specific primer sequences are provided in S1 File.

Western blot

Total protein lysates from tissues were used for analysis. After Western blotting, protein levels were detected using enhanced chemiluminescence (Millipore Merck, Taipei, Taiwan). The primary and secondary antibodies are listed in S1 File.

Adipokine array

Mice were fasted for 18 h before their blood was collected. Serum was isolated, and 100 μL was used in a commercial adipokine array (ARY-013, R&D systems, Minneapolis, MN, USA) according to the manufacturer’s protocol. The proteome profiler adipokine array detects 38 adipokines in duplicate on nitrocellulose membranes as described in the Supplementary Data.

Cell culture, adipocyte differentiation, transfection, and stably transfected cell lines

3T3-L1 preadipocytes were obtained from the Taiwan Bioresource Collection and Research Center (Hsinchu, Taiwan). Generation of the Glut10-knockdown and control stable cell lines was accomplished using pLKO shRNA vectors encoding shRNAs targeting Glut10 or luciferase sequences as previously described [20]. MEFs were isolated from post coitus day 13.5 Glut10G128E and WT embryos as previously described [71]. All MEFs in this study were used within two passages. Cells were cultured and differentiation was induced for the indicated number of days as described previously [16].

Oil Red O staining and quantification

Cells were fixed with 10% formaldehyde at the indicated times after induction of differentiation and were stained with 0.3% Oil Red O (Sigma-Aldrich Inc., St. Louis, MO, USA) in 60% isopropanol. The Oil Red O was dissolved in isopropanol for quantification by absorbance spectrophotometry at 510 nm.

DHA uptake and intracellular AA measurements

DHA uptake was determined using 14C-labeled DHA, and intracellular AA levels were determined using an Ascorbic Acid Assay Kit (Abcam, Cambridge, England, UK).

Global 5hmC quantitation

Genomic DNA was purified and sonicated. Global DNA hydroxymethylation (5hmC) was measured using commercial kits (ab117131, Abcam). The percentage of 5hmC in each sample was calculated. The calibration curve and negative controls were measured in duplicate, while the samples were measured in triplicate.

Gene-specific 5hmC quantitation

Genomic DNA was purified and sonicated. The DNA fragments containing 5hmC were enriched and isolated using commercial kits (ab117134, Abcam), and qPCR was used to quantify the enrichment of 5hmC DNA at specific gene loci. The sequences of primer pairs are listed in the Supplementary Data.

Statistical analysis

A two-tailed Student’s t-test was used to test for differences between groups. P-values less than 0.05 were considered statistically significant.

Supporting information

S1 File [pdf]
Supplementary materials and methods.

S1 Table [pdf]
SNPs in region associated with BMI, glucose tolerance, insulin resistance or T2D-related phenotypes in independent human populations and studies.

S2 Table [xlsx]
The potential variants in SLC2A10 region that might affect the GLUT10 expression or function.

S3 Table [pdf]
Variants in the region

S1 Fig [a]
GLUT10 is conserved during evolution, and the GLUT10 variant is predicted to be benign.

S2 Fig [a]
mice exhibit no significant difference in food intake, physical activity, or energy expenditure.

S3 Fig [mock]
HFD-fed mice have impaired insulin-induced AKT phosphorylation in insulin-responsive tissues.

S4 Fig [a]
The appearance and histology of sWAT, iBAT, and pancreatic tissue of WT and mice after CD or HFD feeding.

S5 Fig [cd]
mice on a CD or HFD exhibit increased inflammation and adipokine dysregulation in eWAT.

S6 Fig [a]
mice have no obvious abnormalities in vasculature on a CD or HFD.

S7 Fig [fc]
Analysis of dysregulated genes in eWAT of GLUT10 variant mice.

S8 Fig [human]
Relative GLUT10 expression in human and mouse tissues.

S9 Fig [a]
The expression of SVCTs and GLUTs in and 3T3-L1 cells.

S10 Fig [a]
Ascorbic acid rescues the adipogenesis ability in MEFs.

S11 Fig [pdf]
GLUT10-deficient 3T3-L1 cells are more sensitive to DFO and DMOG inhibiting adipogenesis.

S12 Fig [cd]
mice on a CD or HFD exhibit disrupted lipid metabolism and adipogenesis in eWAT.

S13 Fig [a]
Ascorbic acid homeostasis is disturbed in mice.

S14 Fig [aa]
Ascorbic acid supplementation improves mitochondrial function and reduces inflammatory cytokine expression in 3T3-L1 preadipocytes.

S15 Fig [aa]
Ascorbic acid enhances adipogenesis in 3T3-L1 cells but other antioxidants do not.

S1 Data [xlsx]
Underlying numerical data corresponding to the main figures.


Zdroje

1. Replication DIG, Meta-analysis C, Asian Genetic Epidemiology Network Type 2 Diabetes C, South Asian Type 2 Diabetes C, Mexican American Type 2 Diabetes C, Type 2 Diabetes Genetic Exploration by Nex-generation sequencing in muylti-Ethnic Samples C, et al. Genome-wide trans-ancestry meta-analysis provides insight into the genetic architecture of type 2 diabetes susceptibility. Nat Genet. 2014;46(3):234–44. doi: 10.1038/ng.2897 24509480; PubMed Central PMCID: PMC3969612.

2. Scott RA, Scott LJ, Magi R, Marullo L, Gaulton KJ, Kaakinen M, et al. An Expanded Genome-Wide Association Study of Type 2 Diabetes in Europeans. Diabetes. 2017;66(11):2888–902. Epub 2017/06/02. doi: 10.2337/db16-1253 28566273; PubMed Central PMCID: PMC5652602.

3. Bonnefond A, Clement N, Fawcett K, Yengo L, Vaillant E, Guillaume JL, et al. Rare MTNR1B variants impairing melatonin receptor 1B function contribute to type 2 diabetes. Nat Genet. 2012;44(3):297–301. Epub 2012/01/31. doi: 10.1038/ng.1053 22286214; PubMed Central PMCID: PMC3773908.

4. Majithia AR, Flannick J, Shahinian P, Guo M, Bray MA, Fontanillas P, et al. Rare variants in PPARG with decreased activity in adipocyte differentiation are associated with increased risk of type 2 diabetes. Proc Natl Acad Sci U S A. 2014;111(36):13127–32. Epub 2014/08/27. doi: 10.1073/pnas.1410428111 25157153; PubMed Central PMCID: PMC4246964.

5. Flannick J, Thorleifsson G, Beer NL, Jacobs SB, Grarup N, Burtt NP, et al. Loss-of-function mutations in SLC30A8 protect against type 2 diabetes. Nat Genet. 2014;46(4):357–63. Epub 2014/03/04. doi: 10.1038/ng.2915 24584071; PubMed Central PMCID: PMC4051628.

6. Garcia-Diaz DF, Lopez-Legarrea P, Quintero P, Martinez JA. Vitamin C in the treatment and/or prevention of obesity. J Nutr Sci Vitaminol (Tokyo). 2014;60(6):367–79. Epub 2014/01/01. doi: 10.3177/jnsv.60.367 25866299.

7. Canoy D, Wareham N, Welch A, Bingham S, Luben R, Day N, et al. Plasma ascorbic acid concentrations and fat distribution in 19,068 British men and women in the European Prospective Investigation into Cancer and Nutrition Norfolk cohort study. Am J Clin Nutr. 2005;82(6):1203–9. Epub 2005/12/08. doi: 10.1093/ajcn/82.6.1203 16332652.

8. Johnston CS, Beezhold BL, Mostow B, Swan PD. Plasma vitamin C is inversely related to body mass index and waist circumference but not to plasma adiponectin in nonsmoking adults. J Nutr. 2007;137(7):1757–62. Epub 2007/06/23. doi: 10.1093/jn/137.7.1757 17585027.

9. Garcia OP, Ronquillo D, Caamano Mdel C, Camacho M, Long KZ, Rosado JL. Zinc, vitamin A, and vitamin C status are associated with leptin concentrations and obesity in Mexican women: results from a cross-sectional study. Nutr Metab (Lond). 2012;9(1):59. Epub 2012/06/19. doi: 10.1186/1743-7075-9-59 22703731; PubMed Central PMCID: PMC3406981.

10. Taylor AE, Ebrahim S, Ben-Shlomo Y, Martin RM, Whincup PH, Yarnell JW, et al. Comparison of the associations of body mass index and measures of central adiposity and fat mass with coronary heart disease, diabetes, and all-cause mortality: a study using data from 4 UK cohorts. Am J Clin Nutr. 2010;91(3):547–56. Epub 2010/01/22. doi: 10.3945/ajcn.2009.28757 20089729.

11. Campion J, Milagro FI, Fernandez D, Martinez JA. Diferential gene expression and adiposity reduction induced by ascorbic acid supplementation in a cafeteria model of obesity. J Physiol Biochem. 2006;62(2):71–80. Epub 2007/01/16. doi: 10.1007/BF03174068 17217161.

12. Lykkesfeldt J, Poulsen HE. Is vitamin C supplementation beneficial? Lessons learned from randomised controlled trials. Br J Nutr. 2010;103(9):1251–9. doi: 10.1017/S0007114509993229 20003627.

13. Kuiper C, Vissers MC. Ascorbate as a co-factor for fe- and 2-oxoglutarate dependent dioxygenases: physiological activity in tumor growth and progression. Front Oncol. 2014;4:359. Epub 2014/12/30. doi: 10.3389/fonc.2014.00359 25540771; PubMed Central PMCID: PMC4261134.

14. Banhegyi G, Benedetti A, Margittai E, Marcolongo P, Fulceri R, Nemeth CE, et al. Subcellular compartmentation of ascorbate and its variation in disease states. Biochim Biophys Acta. 2014;1843(9):1909–16. doi: 10.1016/j.bbamcr.2014.05.016 24907663.

15. Coucke PJ, Willaert A, Wessels MW, Callewaert B, Zoppi N, De Backer J, et al. Mutations in the facilitative glucose transporter GLUT10 alter angiogenesis and cause arterial tortuosity syndrome. Nat Genet. 2006;38(4):452–7. doi: 10.1038/ng1764 16550171.

16. Lee YC, Huang HY, Chang CJ, Cheng CH, Chen YT. Mitochondrial GLUT10 facilitates dehydroascorbic acid import and protects cells against oxidative stress: mechanistic insight into arterial tortuosity syndrome. Hum Mol Genet. 2010;19(19):3721–33. Epub 2010/07/20. ddq286 [pii] doi: 10.1093/hmg/ddq286 20639396.

17. Zoppi N, Chiarelli N, Cinquina V, Ritelli M, Colombi M. GLUT10 deficiency leads to oxidative stress and non-canonical alphavbeta3 integrin-mediated TGFbeta signalling associated with extracellular matrix disarray in arterial tortuosity syndrome skin fibroblasts. Hum Mol Genet. 2015. doi: 10.1093/hmg/ddv382 26376865.

18. Nemeth CE, Marcolongo P, Gamberucci A, Fulceri R, Benedetti A, Zoppi N, et al. Glucose transporter type 10—lacking in arterial tortuosity syndrome—facilitates dehydroascorbic acid transport. FEBS Lett. 2016. doi: 10.1002/1873-3468.12204 27153185.

19. Gamberucci A, Marcolongo P, Nemeth CE, Zoppi N, Szarka A, Chiarelli N, et al. GLUT10-Lacking in Arterial Tortuosity Syndrome-Is Localized to the Endoplasmic Reticulum of Human Fibroblasts. Int J Mol Sci. 2017;18(8). Epub 2017/08/23. doi: 10.3390/ijms18081820 28829359; PubMed Central PMCID: PMC5578206.

20. Syu YW, Lai HW, Jiang CL, Tsai HY, Lin CC, Lee YC. GLUT10 maintains the integrity of major arteries through regulation of redox homeostasis and mitochondrial function. Hum Mol Genet. 2018;27(2):307–21. doi: 10.1093/hmg/ddx401 29149261.

21. Willaert A, Khatri S, Callewaert BL, Coucke PJ, Crosby SD, Lee JG, et al. GLUT10 is required for the development of the cardiovascular system and the notochord and connects mitochondrial function to TGFbeta signaling. Hum Mol Genet. 2012;21(6):1248–59. doi: 10.1093/hmg/ddr555 22116938; PubMed Central PMCID: PMC3284116.

22. Ghosh S, Watanabe RM, Hauser ER, Valle T, Magnuson VL, Erdos MR, et al. Type 2 diabetes: evidence for linkage on chromosome 20 in 716 Finnish affected sib pairs. Proc Natl Acad Sci U S A. 1999;96(5):2198–203. doi: 10.1073/pnas.96.5.2198 10051618; PubMed Central PMCID: PMC26760.

23. Zouali H, Hani EH, Philippi A, Vionnet N, Beckmann JS, Demenais F, et al. A susceptibility locus for early-onset non-insulin dependent (type 2) diabetes mellitus maps to chromosome 20q, proximal to the phosphoenolpyruvate carboxykinase gene. Hum Mol Genet. 1997;6(9):1401–8. doi: 10.1093/hmg/6.9.1401 9285775.

24. Andersen G, Rose CS, Hamid YH, Drivsholm T, Borch-Johnsen K, Hansen T, et al. Genetic variation of the GLUT10 glucose transporter (SLC2A10) and relationships to type 2 diabetes and intermediary traits. Diabetes. 2003;52(9):2445–8. Epub 2003/08/28. doi: 10.2337/diabetes.52.9.2445 12941788.

25. Bento JL, Bowden DW, Mychaleckyj JC, Hirakawa S, Rich SS, Freedman BI, et al. Genetic analysis of the GLUT10 glucose transporter (SLC2A10) polymorphisms in Caucasian American type 2 diabetes. BMC Med Genet. 2005;6:42. doi: 10.1186/1471-2350-6-42 16336637.

26. Mohlke KL, Skol AD, Scott LJ, Valle TT, Bergman RN, Tuomilehto J, et al. Evaluation of SLC2A10 (GLUT10) as a candidate gene for type 2 diabetes and related traits in Finns. Mol Genet Metab. 2005;85(4):323–7. doi: 10.1016/j.ymgme.2005.04.011 15936967.

27. Rose CS, Andersen G, Hamid YH, Glumer C, Drivsholm T, Borch-Johnsen K, et al. Studies of relationships between the GLUT10 Ala206Thr polymorphism and impaired insulin secretion. Diabet Med. 2005;22(7):946–9. doi: 10.1111/j.1464-5491.2005.01547.x 15975113.

28. Lin WH, Chuang LM, Chen CH, Yeh JI, Hsieh PS, Cheng CH, et al. Association study of genetic polymorphisms of SLC2A10 gene and type 2 diabetes in the Taiwanese population. Diabetologia. 2006;49(6):1214–21. doi: 10.1007/s00125-006-0218-3 16586067.

29. Jiang YD, Chang YC, Chiu YF, Chang TJ, Li HY, Lin WH, et al. SLC2A10 genetic polymorphism predicts development of peripheral arterial disease in patients with type 2 diabetes. SLC2A10 and PAD in type 2 diabetes. BMC Med Genet. 2010;11:126. doi: 10.1186/1471-2350-11-126 20735855; PubMed Central PMCID: PMC2939510.

30. Wood IS, Hunter L, Trayhurn P. Expression of Class III facilitative glucose transporter genes (GLUT-10 and GLUT-12) in mouse and human adipose tissues. Biochem Biophys Res Commun. 2003;308(1):43–9. doi: 10.1016/s0006-291x(03)01322-6 12890477.

31. Lago F, Dieguez C, Gomez-Reino J, Gualillo O. Adipokines as emerging mediators of immune response and inflammation. Nat Clin Pract Rheumatol. 2007;3(12):716–24. doi: 10.1038/ncprheum0674 18037931.

32. Dupuis J, Langenberg C, Prokopenko I, Saxena R, Soranzo N, Jackson AU, et al. New genetic loci implicated in fasting glucose homeostasis and their impact on type 2 diabetes risk. Nat Genet. 2010;42(2):105–16. Epub 2010/01/19. doi: 10.1038/ng.520 20081858; PubMed Central PMCID: PMC3018764.

33. Florkowski C. HbA1c as a Diagnostic Test for Diabetes Mellitus—Reviewing the Evidence. Clin Biochem Rev. 2013;34(2):75–83. Epub 2013/10/24. 24151343; PubMed Central PMCID: PMC3799221.

34. Halperin E, Stephan DA. SNP imputation in association studies. Nat Biotechnol. 2009;27(4):349–51. Epub 2009/04/09. doi: 10.1038/nbt0409-349 19352374.

35. Chen P, Takeuchi F, Lee JY, Li H, Wu JY, Liang J, et al. Multiple nonglycemic genomic loci are newly associated with blood level of glycated hemoglobin in East Asians. Diabetes. 2014;63(7):2551–62. Epub 2014/03/22. doi: 10.2337/db13-1815 24647736; PubMed Central PMCID: PMC4284402.

36. Cheng CH, Kikuchi T, Chen YH, Sabbagha NG, Lee YC, Pan HJ, et al. Mutations in the SLC2A10 gene cause arterial abnormalities in mice. Cardiovasc Res. 2009;81(2):381–8. doi: 10.1093/cvr/cvn319 19028722.

37. Lancha A, Fruhbeck G, Gomez-Ambrosi J. Peripheral signalling involved in energy homeostasis control. Nutr Res Rev. 2012;25(2):223–48. Epub 2012/11/24. doi: 10.1017/S0954422412000145 23174510.

38. Sun K, Kusminski CM, Scherer PE. Adipose tissue remodeling and obesity. J Clin Invest. 2011;121(6):2094–101. Epub 2011/06/03. doi: 10.1172/JCI45887 21633177; PubMed Central PMCID: PMC3104761.

39. Olefsky JM, Glass CK. Macrophages, inflammation, and insulin resistance. Annu Rev Physiol. 2010;72:219–46. Epub 2010/02/13. doi: 10.1146/annurev-physiol-021909-135846 20148674.

40. Suganami T, Tanaka M, Ogawa Y. Adipose tissue inflammation and ectopic lipid accumulation. Endocr J. 2012;59(10):849–57. Epub 2012/08/11. doi: 10.1507/endocrj.ej12-0271 22878669.

41. Tang W, Zeve D, Suh JM, Bosnakovski D, Kyba M, Hammer RE, et al. White fat progenitor cells reside in the adipose vasculature. Science. 2008;322(5901):583–6. doi: 10.1126/science.1156232 18801968; PubMed Central PMCID: PMC2597101.

42. Blaschke K, Ebata KT, Karimi MM, Zepeda-Martinez JA, Goyal P, Mahapatra S, et al. Vitamin C induces Tet-dependent DNA demethylation and a blastocyst-like state in ES cells. Nature. 2013;500(7461):222–6. doi: 10.1038/nature12362 23812591; PubMed Central PMCID: PMC3893718.

43. Chen J, Guo L, Zhang L, Wu H, Yang J, Liu H, et al. Vitamin C modulates TET1 function during somatic cell reprogramming. Nat Genet. 2013;45(12):1504–9. Epub 2013/10/29. doi: 10.1038/ng.2807 24162740.

44. Monfort A, Wutz A. Breathing-in epigenetic change with vitamin C. EMBO Rep. 2013;14(4):337–46. doi: 10.1038/embor.2013.29 23492828; PubMed Central PMCID: PMC3615655.

45. Young JI, Zuchner S, Wang G. Regulation of the Epigenome by Vitamin C. Annu Rev Nutr. 2015;35:545–64. doi: 10.1146/annurev-nutr-071714-034228 25974700; PubMed Central PMCID: PMC4506708.

46. Rosen ED, MacDougald OA. Adipocyte differentiation from the inside out. Nat Rev Mol Cell Biol. 2006;7(12):885–96. doi: 10.1038/nrm2066 17139329.

47. Ngo S, Li X, O'Neill R, Bhoothpur C, Gluckman P, Sheppard A. Elevated S-adenosylhomocysteine alters adipocyte functionality with corresponding changes in gene expression and associated epigenetic marks. Diabetes. 2014;63(7):2273–83. Epub 2014/02/28. doi: 10.2337/db13-1640 24574043.

48. Yoo Y, Park JH, Weigel C, Liesenfeld DB, Weichenhan D, Plass C, et al. TET-mediated hydroxymethylcytosine at the Ppargamma locus is required for initiation of adipogenic differentiation. Int J Obes (Lond). 2017;41(4):652–9. Epub 2017/01/20. doi: 10.1038/ijo.2017.8 28100914.

49. Flannick J, Florez JC. Type 2 diabetes: genetic data sharing to advance complex disease research. Nat Rev Genet. 2016;17(9):535–49. Epub 2016/07/13. doi: 10.1038/nrg.2016.56 27402621.

50. Callewaert BL, Willaert A, Kerstjens-Frederikse WS, De Backer J, Devriendt K, Albrecht B, et al. Arterial tortuosity syndrome: clinical and molecular findings in 12 newly identified families. Hum Mutat. 2008;29(1):150–8. doi: 10.1002/humu.20623 17935213.

51. Ritelli M, Chiarelli N, Dordoni C, Reffo E, Venturini M, Quinzani S, et al. Arterial Tortuosity Syndrome: homozygosity for two novel and one recurrent SLC2A10 missense mutations in three families with severe cardiopulmonary complications in infancy and a literature review. BMC Med Genet. 2014;15:122. doi: 10.1186/s12881-014-0122-5 25373504; PubMed Central PMCID: PMC4412100.

52. Beyens A, Albuisson J, Boel A, Al-Essa M, Al-Manea W, Bonnet D, et al. Arterial tortuosity syndrome: 40 new families and literature review. Genet Med. 2018;20(10):1236–45. Epub 2018/01/13. doi: 10.1038/gim.2017.253 29323665.

53. Wessels MW, Catsman-Berrevoets CE, Mancini GM, Breuning MH, Hoogeboom JJ, Stroink H, et al. Three new families with arterial tortuosity syndrome. Am J Med Genet A. 2004;131(2):134–43. doi: 10.1002/ajmg.a.30272 15529317.

54. Franceschini P, Guala A, Licata D, Di Cara G, Franceschini D. Arterial tortuosity syndrome. Am J Med Genet. 2000;91(2):141–3. Epub 2000/04/05. 10748415.

55. Drera B, Guala A, Zoppi N, Gardella R, Franceschini P, Barlati S, et al. Two novel SLC2A10/GLUT10 mutations in a patient with arterial tortuosity syndrome. Am J Med Genet A. 2007;143A(2):216–8. Epub 2006/12/14. doi: 10.1002/ajmg.a.31514 17163528.

56. Huang-Doran I, Sleigh A, Rochford JJ, O'Rahilly S, Savage DB. Lipodystrophy: metabolic insights from a rare disorder. J Endocrinol. 2010;207(3):245–55. Epub 2010/09/28. doi: 10.1677/JOE-10-0272 20870709.

57. Fiorenza CG, Chou SH, Mantzoros CS. Lipodystrophy: pathophysiology and advances in treatment. Nat Rev Endocrinol. 2011;7(3):137–50. doi: 10.1038/nrendo.2010.199 21079616; PubMed Central PMCID: PMC3150735.

58. Nigro E, Scudiero O, Monaco ML, Palmieri A, Mazzarella G, Costagliola C, et al. New insight into adiponectin role in obesity and obesity-related diseases. Biomed Res Int. 2014;2014:658913. Epub 2014/08/12. doi: 10.1155/2014/658913 25110685; PubMed Central PMCID: PMC4109424.

59. Ryo M, Nakamura T, Kihara S, Kumada M, Shibazaki S, Takahashi M, et al. Adiponectin as a biomarker of the metabolic syndrome. Circ J. 2004;68(11):975–81. Epub 2004/10/27. doi: 10.1253/circj.68.975 15502375.

60. Simons PJ, van den Pangaart PS, Aerts JM, Boon L. Pro-inflammatory delipidizing cytokines reduce adiponectin secretion from human adipocytes without affecting adiponectin oligomerization. J Endocrinol. 2007;192(2):289–99. Epub 2007/02/07. doi: 10.1677/JOE-06-0047 17283229.

61. Reilly SM, Saltiel AR. Adapting to obesity with adipose tissue inflammation. Nat Rev Endocrinol. 2017;13(11):633–43. Epub 2017/08/12. doi: 10.1038/nrendo.2017.90 28799554.

62. Hotamisligil GS. Inflammation and metabolic disorders. Nature. 2006;444(7121):860–7. doi: 10.1038/nature05485 17167474.

63. Malandrino MI, Fucho R, Weber M, Calderon-Dominguez M, Mir JF, Valcarcel L, et al. Enhanced fatty acid oxidation in adipocytes and macrophages reduces lipid-induced triglyceride accumulation and inflammation. Am J Physiol Endocrinol Metab. 2015;308(9):E756–69. Epub 2015/02/26. doi: 10.1152/ajpendo.00362.2014 25714670.

64. Patel P, Abate N. Role of subcutaneous adipose tissue in the pathogenesis of insulin resistance. J Obes. 2013;2013:489187. Epub 2013/05/22. doi: 10.1155/2013/489187 23691287; PubMed Central PMCID: PMC3649613.

65. Shi DQ, Ali I, Tang J, Yang WC. New Insights into 5hmC DNA Modification: Generation, Distribution and Function. Front Genet. 2017;8:100. Epub 2017/08/05. doi: 10.3389/fgene.2017.00100 28769976; PubMed Central PMCID: PMC5515870.

66. Cuaranta-Monroy I, Simandi Z, Kolostyak Z, Doan-Xuan QM, Poliska S, Horvath A, et al. Highly efficient differentiation of embryonic stem cells into adipocytes by ascorbic acid. Stem Cell Res. 2014;13(1):88–97. Epub 2014/05/27. doi: 10.1016/j.scr.2014.04.015 24858493.

67. De Pauw A, Tejerina S, Raes M, Keijer J, Arnould T. Mitochondrial (dys)function in adipocyte (de)differentiation and systemic metabolic alterations. Am J Pathol. 2009;175(3):927–39. doi: 10.2353/ajpath.2009.081155 19700756; PubMed Central PMCID: PMC2731113.

68. Koh EH, Park JY, Park HS, Jeon MJ, Ryu JW, Kim M, et al. Essential role of mitochondrial function in adiponectin synthesis in adipocytes. Diabetes. 2007;56(12):2973–81. Epub 2007/09/11. doi: 10.2337/db07-0510 17827403.

69. Huh JY, Kim Y, Jeong J, Park J, Kim I, Huh KH, et al. Peroxiredoxin 3 is a key molecule regulating adipocyte oxidative stress, mitochondrial biogenesis, and adipokine expression. Antioxid Redox Signal. 2012;16(3):229–43. Epub 2011/09/10. doi: 10.1089/ars.2011.3952 21902452; PubMed Central PMCID: PMC3234662.

70. Kusminski CM, Scherer PE. Mitochondrial dysfunction in white adipose tissue. Trends Endocrinol Metab. 2012;23(9):435–43. doi: 10.1016/j.tem.2012.06.004 22784416; PubMed Central PMCID: PMC3430798.

71. Garfield AS. Derivation of primary mouse embryonic fibroblast (PMEF) cultures. Methods Mol Biol. 2010;633:19–27. Epub 2010/03/06. doi: 10.1007/978-1-59745-019-5_2 20204617.


Článek vyšel v časopise

PLOS Genetics


2020 Číslo 5
Nejčtenější tento týden
Nejčtenější v tomto čísle
Kurzy

Zvyšte si kvalifikaci online z pohodlí domova

Důležitost adherence při depresivním onemocnění
nový kurz
Autoři: MUDr. Eliška Bartečková, Ph.D.

Koncepce osteologické péče pro gynekology a praktické lékaře
Autoři: MUDr. František Šenk

Sekvenční léčba schizofrenie
Autoři: MUDr. Jana Hořínková, Ph.D.

Hypertenze a hypercholesterolémie – synergický efekt léčby
Autoři: prof. MUDr. Hana Rosolová, DrSc.

Multidisciplinární zkušenosti u pacientů s diabetem
Autoři: Prof. MUDr. Martin Haluzík, DrSc., prof. MUDr. Vojtěch Melenovský, CSc., prof. MUDr. Vladimír Tesař, DrSc.

Všechny kurzy
Přihlášení
Zapomenuté heslo

Zadejte e-mailovou adresu, se kterou jste vytvářel(a) účet, budou Vám na ni zaslány informace k nastavení nového hesla.

Přihlášení

Nemáte účet?  Registrujte se

#ADS_BOTTOM_SCRIPTS#