Modulation of flagellar rotation in surface-attached bacteria: A pathway for rapid surface-sensing after flagellar attachment

English version

Autoři: Maren Schniederberend ^aff001; Jessica F. Williams ^aff002; Emilee Shine ^aff003; Cong Shen ^aff003; Ruchi Jain ^aff001; Thierry Emonet ^aff002; Barbara I. Kazmierczak ^aff001
Působiště autorů: Department of Medicine (Infectious Diseases), Yale University, New Haven, Connecticut, United States of America ^aff001; Department of Molecular, Cellular & Developmental Biology, Yale University, New Haven, Connecticut, United States of America ^aff002; Program in Microbiology, Yale University, New Haven, Connecticut, United States of America ^aff003; Department of Physics, Yale University, New Haven, Connecticut, United States of America ^aff004; Department of Microbial Pathogenesis, Yale University, New Haven, Connecticut, United States of America ^aff005
Vyšlo v časopise: Modulation of flagellar rotation in surface-attached bacteria: A pathway for rapid surface-sensing after flagellar attachment. PLoS Pathog 15(11): e32767. doi:10.1371/journal.ppat.1008149
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.ppat.1008149

Souhrn

Attachment is a necessary first step in bacterial commitment to surface-associated behaviors that include colonization, biofilm formation, and host-directed virulence. The Gram-negative opportunistic pathogen Pseudomonas aeruginosa can initially attach to surfaces via its single polar flagellum. Although many bacteria quickly detach, some become irreversibly attached and express surface-associated structures, such as Type IV pili, and behaviors, including twitching motility and biofilm initiation. P. aeruginosa that lack the GTPase FlhF assemble a randomly placed flagellum that is motile; however, we observed that these mutant bacteria show defects in biofilm formation comparable to those seen for non-motile, aflagellate bacteria. This phenotype was associated with altered behavior of ΔflhF bacteria immediately following surface-attachment. Forward and reverse genetic screens led to the discovery that FlhF interacts with FimV to control flagellar rotation at a surface, and implicated cAMP signaling in this pathway. Although cAMP controls many transcriptional programs in P. aeruginosa, known targets of this second messenger were not required to modulate flagellar rotation in surface-attached bacteria. Instead, alterations in switching behavior of the motor appeared to result from direct or indirect effects of cAMP on switch complex proteins and/or the stators associated with them.

Klíčová slova:

Bacterial biofilms – Flagella – Intracellular pathogens – Pathogen motility – Pseudomonas aeruginosa – Swimming – Flagellar rotation – Adenylyl cyclase

Zdroje

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