Oxamniquine resistance alleles are widespread in Old World Schistosoma mansoni and predate drug deployment
Authors:
Frédéric D. Chevalier aff001; Winka Le Clec’h aff001; Marina McDew-White aff001; Vinay Menon aff001; Meghan A. Guzman aff002; Stephen P. Holloway aff003; Xiaohang Cao aff003; Alexander B. Taylor aff003; Safari Kinung'hi aff005; Anouk N. Gouvras aff006; Bonnie L. Webster aff006; Joanne P. Webster aff006; Aidan M. Emery aff006; David Rollinson aff006; Amadou Garba Djirmay aff009; Khalid M. Al Mashikhi aff011; Salem Al Yafae aff011; Mohamed A. Idris aff012; Hélène Moné aff013; Gabriel Mouahid aff013; P. John Hart aff003; Philip T. LoVerde aff002; Timothy J. C. Anderson aff001
Authors place of work:
Texas Biomedical Research Institute, San Antonio, Texas, United States of America
aff001; Departments of Pathology and University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States of America
aff002; Biochemistry & Structural Biology, University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States of America
aff003; X-ray Crystallography Core Laboratory, University of Texas Health Science Center at San Antonio, San Antonio, Texas, United States of America
aff004; National Institute for Medical Research, Mwanza, United Republic of Tanzania
aff005; London Centre for Neglected Tropical Disease Research (LCNDTR), Imperial Collge, London, United Kingdom
aff006; Wolfson Wellcome Biomedical Laboratories, Natural History Museum, London, United Kingdom
aff007; Centre for Emerging, Endemic and Exotic Diseases (CEEED), Royal Veterinary College, University of London, United Kingdom
aff008; Réseau International Schistosomiases Environnemental Aménagement et Lutte (RISEAL), Niamey, Niger
aff009; World Health Organization, Geneva, Switzerland
aff010; Directorate General of Health Services, Dhofar Governorate, Salalah, Sultanate of Oman
aff011; Sultan Qaboos University, Muscat, Sultanate of Oman
aff012; Host-Pathogen-Environment Interactions laboratory, University of Perpignan, Perpignan, France
aff013
Published in the journal:
Oxamniquine resistance alleles are widespread in Old World Schistosoma mansoni and predate drug deployment. PLoS Pathog 15(10): e32767. doi:10.1371/journal.ppat.1007881
Category:
Research Article
doi:
https://doi.org/10.1371/journal.ppat.1007881
Summary
Do mutations required for adaptation occur de novo, or are they segregating within populations as standing genetic variation? This question is key to understanding adaptive change in nature, and has important practical consequences for the evolution of drug resistance. We provide evidence that alleles conferring resistance to oxamniquine (OXA), an antischistosomal drug, are widespread in natural parasite populations under minimal drug pressure and predate OXA deployment. OXA has been used since the 1970s to treat Schistosoma mansoni infections in the New World where S. mansoni established during the slave trade. Recessive loss-of-function mutations within a parasite sulfotransferase (SmSULT-OR) underlie resistance, and several verified resistance mutations, including a deletion (p.E142del), have been identified in the New World. Here we investigate sequence variation in SmSULT-OR in S. mansoni from the Old World, where OXA has seen minimal usage. We sequenced exomes of 204 S. mansoni parasites from West Africa, East Africa and the Middle East, and scored variants in SmSULT-OR and flanking regions. We identified 39 non-synonymous SNPs, 4 deletions, 1 duplication and 1 premature stop codon in the SmSULT-OR coding sequence, including one confirmed resistance deletion (p.E142del). We expressed recombinant proteins and used an in vitro OXA activation assay to functionally validate the OXA-resistance phenotype for four predicted OXA-resistance mutations. Three aspects of the data are of particular interest: (i) segregating OXA-resistance alleles are widespread in Old World populations (4.29–14.91% frequency), despite minimal OXA usage, (ii) two OXA-resistance mutations (p.W120R, p.N171IfsX28) are particularly common (>5%) in East African and Middle-Eastern populations, (iii) the p.E142del allele has identical flanking SNPs in both West Africa and Puerto Rico, suggesting that parasites bearing this allele colonized the New World during the slave trade and therefore predate OXA deployment. We conclude that standing variation for OXA resistance is widespread in S. mansoni.
Keywords:
Haplotypes – Alleles – Mutation – Africa – Parasitic diseases – Deletion mutation – Schistosoma mansoni – Schistosoma
Introduction
The rate at which drug resistance alleles (or any other beneficial alleles) spread within populations in response to a drug treatment (or any other selection pressure) is critically dependent on the starting frequency of resistance alleles in the population when new drugs are deployed [1,2]. If no such alleles are present when a novel drug is introduced, then there is a waiting time for resistance alleles to arise. Furthermore, because the starting allele frequency will be 1/(2Ne), where Ne is the effective population size, the vast majority of resistance alleles that arise will be lost due to genetic drift and fail to establish [2,3] (Fig 1). The barrier to establishment is particularly severe for recessive traits because these will be present in heterozygotes at low frequency and therefore not exposed to selection. This effect, otherwise known as “Haldanes’s sieve” [4], may be particularly relevant for drug resistance evolution in diploid pathogens, because resistance mutations in drug targets typically result in phenotypic resistance only when two copies are present. However, if resistance alleles are already segregating as standing variation in pathogen populations when drug treatment is initiated, then there is no waiting time for mutations to arise. Furthermore, there is a high probability of fixation and spread is rapid because many resistance alleles are present in homozygous recessive genotypes and therefore exposed to selection. Hence, whether resistance alleles (or alleles for other traits) arise de novo or are segregating as standing variation, is a central question in evolutionary biology and public health, and key to predicting the effective “shelf life” of new drug treatments [3,5].
There is growing evidence for resistance to anti-helminthics in several helminths infecting humans, including Onchocerca volvulus [6], the filarial nematode causing river blindness, and in soil transmitted helminths (hookworm, whipworm and Ascaris roundworms) [7] which cumulatively infect over one billion people worldwide [8]. However, perhaps the best understood is oxamniquine resistance in schistosome blood flukes, for which the mechanism of drug action and the genetic and molecular basis for drug resistance are now known. Oxamniquine (OXA) kills Schistosoma mansoni, but is ineffective against two other major schistosome species infecting humans (S. haematobium and S. japonicum) [9]. OXA is a pro-drug that is activated by a schistosome sulfotransferase (SmSULT-OR) encoded on chromosome 6 [10]. Loss-of-function mutations within SmSULT-OR result in resistance: only parasites that are homozygous for resistance alleles are phenotypically resistant (OXA-R). We initially identified the locus and mutation, a single amino acid deletion (p.E142del), underlying OXA-R using a genetic cross involving a resistant parasite line selected from a parasite isolate from a Puerto Rican patient in 1971 [11]. Additionally we identified a second mutation (p.C35R) in S. mansoni obtained from an incurable Brazilian patient [12]. These two mutations result in disruption of the active site of the SmSULT-OR and inability to activate OXA [10]. Both p.E142del and p.C35R, and two additional mutations resulting in truncated proteins, were subsequently identified in a field survey of SmSULT-OR genetic variation of parasites from a single Brazilian village [13]. The combined allele frequency of these four resistance alleles was 1.85%.
OXA was widely used in the New World, where only S. mansoni is present, during the 1970s to early 2000’s [14], so the OXA-R alleles we observed in Brazil [13] could conceivably have resulted from drug selection. In contrast, OXA saw minimal usage in Africa where both S. haematobium and S. mansoni are present. In South America, an estimated 6 million doses of OXA were used to treat the 7 million people infected with S. mansoni prior to 1987 [15]. In contrast, 3 million doses had been used in Africa (Egypt, Ethiopia, Ivory Coast, Kenya, Malagasy, Malawi, Rwanda-Burundi, South Africa, Sudan, Tanzania, Uganda, Zambia, Zaire) and the Middle East (Arabian penensula) [15], where an estimated ~60 million people were infected with S. mansoni [16]. Hence, OXA selection was minimal in Africa compared with South America. The central aim of this paper is to determine whether de novo mutation or standing variation best explains OXA-R in S. mansoni populations. To do this we examined SmSULT-OR genetic variation in Old World parasite populations where OXA treatment has been minimal.
Results
Samples
We sequenced exomes from 92 miracidia from Tanzania (n = 57), Niger (n = 10), and Senegal (n = 25) from the SCAN collection at the Natural History Museum [17], with each miracidium derived from a different patient. In addition, we included 112 samples collected from Oman: these included 86 single worms and 26 pools of cercariae from single infected snails. The Omani samples were derived from 11 human infections, 2 naturally infected rodents, and 26 naturally infected snails (Table 1). We obtained an average of 15.7 ± 8 (mean ± standard deviation, s.d.) million reads per library (S1 Table). A very high proportion of these reads were mapped to the reference genome (91.9 ± 6.29 (s.d.) % on average). We captured on average 99.08 ± 3.92 (s.d.) % of the bait regions. We obtained an average read depth of 64.40 ± 32.27 (s.d.) in the bait regions. For SmSULT-OR, we obtained sequence across 95.06 ± 6.54 (s.d.) % of the coding sequence with an average read depth of 43.09 ± 26.96 (s.d.).
We compared these Old World parasite samples with: (i) the OXA-R parasite from Puerto Rico (HR) carrying the causative p.E142del mutation identified previously [10], for which whole genome sequence is available, and (ii) published SmSULT-OR sequences from a single Brazilian location (n = 189) [13] (Table 1).
New and known mutations identified in the SmSULT-OR gene
We identified a total of 85 SmSULT-OR mutations across all three populations (West Africa, East Africa and Middle East), including 76 coding mutations and 9 non-coding mutations (S2 Table). Exon 1 carried 24 mutations. These included 13 non-synonymous single nucleotide polymorphisms (SNPs), 8 synonymous SNPs, one duplication, one deletion, and one premature stop codon. Exon 2 carried 52 mutations: 26 non-synonymous SNPs, 23 synonymous and three deletions. There were no differences in the density of mutations in the two exons (Fisher’s exact test, d.f. = 1, p = 0.09). The overall transition/transversion ratio was 1.94. Forty-three percent (37/85 mutations) showed an average allele frequency greater than 5%.
Among the 85 mutations, we have previously found three coding and one non-coding mutations in the New World (Caribbean and Brazil): the substitution p.P67L (g.200C>T), the deletion p.E142del (g.4348_4350delGAA), the substitution p.L256W (g.4691T>G) and the 3’ untranslated region (UTR) substitution g.4720C>T [10,13]. Just one of these mutations (p.E142del) encodes a confirmed OXA-R allele. Frequencies of all mutations in each population are shown in S2 Table.
We measured nucleotide diversity (π) in both New and Old World populations. Nucleotide diversity was the highest in Oman (π = 247.5 ± 3.4 × 10−4) followed by East Africa (π = 22.8 ± 2.3 × 10−4), West Africa (π = 7.2 ± 1.5 × 10−4) and Brazil (π = 2.1 ± 0.3 × 10−4). If drug pressure drives the changes at SmSULT-OR, we might expect to see signals of positive selection. We examined sequence conservation by measuring proportions of non-synonymous difference per non-synonymous site relative to synonymous changes per synonymous site (Ka/Ks) between S. mansoni and a S. rodhaini outgroup. Ka/Ks ranged from 0.20–0.24 suggesting that changes at non-synonymous sites are 4–5 times less abundant than at synonymous sites, consistent with weak purifying selection. Similarly, the ratio of non-synonymous polymorphisms per non-synonymous site relative to synonymous polymorphisms per synonymous site (pN/pS) ranged from 0.11 to 1.23 and none of these values were significantly greater than unity (Table 2). These tests did not provide evidence that SmSULT-OR is under positive selection.
In vitro tests and in silico evaluation of impact of mutations
We used three approaches to identify SmSULT-OR mutations that are likely to result in OXA-R.
Visual assessment
Some mutations have obvious deleterious effects on the protein by introducing premature stop codons. This is the case for three mutations identified in SmSULT-OR (Fig 2). These mutations will generate truncated proteins that do not contain the active site. Other mutations, such as p.L179P, p.P225S or p.W120R, were either close enough to the OXA or the 3’-phosphoadenosine-5’-phosphosulfate (PAPS) binding sites or showed potential steric hindrance or structural deformation likely to have an effect on protein activity. Other mutations, such as p.A74T, p.P106S or p.Q176R, were on the surface of the protein, distant to any active site, and therefore unlikely to have an impact.
Functional assay of OXA binding
We used an in vitro OXA activation assay to functionally assay the impact of mutations on SmSULT-OR activity for four newly discovered mutations and the two known OXA-R mutations (Fig 3). The known resistant mutations, p.C35R and p.E142del, showed from zero to very low level of OXA activation, as expected [10]. p.L179P showed a similarly low level of activation. The mutations p.S160L and p.P225S showed intermediate OXA activation: we conservatively classed these as OXA sensitive alleles. p.P106S showed similar activity to the wild type and p.S160L. We were able to produce recombinant protein p.W120R but all our attempts to fold this protein were unsuccessful. This mutation clearly has a dramatic impact on protein stability as predicted by visual inspection and thermodynamic modelling results (below). We considered this mutation to be OXA-R.
Thermodynamic modelling
We used thermodynamic modelling to evaluate the potential impact of substitutions on protein stability (indels such as p.E142del cannot be modelled). The difference in free enthalpy (ΔΔG) ranged from -2.942 to 19.826 for the 37 mutations (Fig 4). Among those, p.L179P and p.C35R which have the greatest impact on OXA activation (Fig 3) showed the second and third highest ΔΔG, respectively. p.W120R, for which we were unable to fold recombinant protein, showed the highest ΔΔG, consistent with a dramatic impact on stability. p.S160L and p.P225S showed intermediate ΔΔG consistent with the results of the OXA activation assay.
In total, we identified 6 independent OXA-R mutations in Old World parasite populations examined using these three approaches (Table 3, Fig 2). These included 1 duplication, 1 known indel, 2 new indels and 3 SNPs.
Frequency of OXA-R alleles
Having determined which of the mutations identified are likely to cause OXA-R, we measured the frequency of resistance variants and the frequency of resistant parasites in each population (Table 3, Fig 5). To accurately identify resistance alleles and parasites, we phased our variant calling data on the first 3 Mb of the chromosome 6 (corresponding to 30,812 variable sites). West African samples carried only two resistance variants (p.S12X and p.E142del). East African samples carried five resistance variants, two of them at a frequency over 0.05 (p.W120R and p.N171IfsX28). The p.W120R mutation was also found at high frequency in Middle East (0.065). We found the highest frequency of OXA-R alleles in East Africa (14.91%, 17/114), followed by the Middle East (6.25%, 14/224), West Africa (4.29%, 3/70), and Brazil (1.85%, 7/378).
Most parasites carried OXA-R alleles in the heterozygous state and are predicted to be OXA sensitive. Heterozygous OXA-sensitive parasites were found in West and East Africa (8.6% and 19.3%, respectively), Middle East (12.5%) and in Brazil (1.6%). Parasites that are homozygous for OXA-R alleles at SmSULT-OR (but not necessarily carrying the same resistance variant) are OXA-R. We found homozygous parasites predicted to be phenotypically OXA-R at a frequency of 5.26% (3/57) in East Africa and 1.06% (2/189) in South America only (Fig 5).
Haplotype analysis of SmSULT-OR and flanking regions
We used our phased data to investigate the haplotypes surrounding our resistance variant (p.E142del) in the Old and New World (Caribbean) (Table 1) and to investigate whether these resistance alleles derived from a common ancestor. This is of interest because it would suggest that these alleles predate the slave trade. We identified an identical 102.5 kb haplotype block around p.E142del shared between the Caribbean sample and one of the West African samples from Niger (Fig 6A). This haplotype block contained 399 SNPs that varied in at least one of the 205 parasites examined (all samples except those from Brazil where haplotype data were not available). We used a minimum spanning network to investigate haplotype relationships using the 399 bi-allelic variants found in this 102.5 kb block across all phased haplotypes (Fig 6B). The haplotypes associated with the p.E142del OXA-R alleles found in both Puerto Rico and Niger clustered together, consistent with a common origin for West African and Caribbean p.E142del OXA-R mutations.
The p.E142del mutation was also identified in Brazil [13] but we sequenced the SmSULT-OR exons only, rather than complete exomes, for these Brazilian samples preventing reconstruction of extended haplotypes. This Brazilian p.E142del was also found associated with two mutations (p.P67L and g.4720C>T) found in West Africa but without the p.L256W found in samples from the Caribbean and Niger. Similarly, the p.E142del was also found in a Tanzanian sample but this sample had a distinct haplotype from those found in West Africa (Fig 6). These results suggest either that there are several independent origins of East and West African p.E142del mutations, or alternatively, that the p.E142del is extremely old and has distinctive flanking haplotypes resulting from recombination and mutation.
Discussion
Evidence for standing genetic variation for OXA resistance
Two lines of evidence support the view that standing variation is the source of OXA-R alleles in schistosomes. First, we have indirect evidence: OXA-R alleles are geographically widespread in Africa and the Middle East despite limited treatment with OXA (or hycanthone, its structural analog; [18]) in these regions. Furthermore, that OXA-R alleles are found at high frequency in East Africa, where there has been minimal use of OXA, while OXA-R alleles are found at low frequency in Brazil, where OXA has been used extensively, is also consistent with the idea that mutation and drift, rather than selection, explain the patterns of variation observed. The second line of evidence is more direct: we showed that an OXA-R mutation, p.E142del, with an identical 102.5 kb flanking haplotype, is sampled from both West African and Caribbean schistosomes. This result strongly suggests that p.E142del alleles were present in West Africa prior to the transatlantic slave trade (1501–1867) [19], and were transferred to the New World on ships carrying West African slaves. Hence, OXA-R alleles were segregating within S. mansoni populations at least 470 years before deployment of OXA in the 1970s [20].
Our molecular data are backed up by clinical observations from the early use of OXA. Resistant parasites were detected in Brazil in the 1970s [21,22], before any mass drug administration [14]. Similar observations of S. mansoni infected patients resistant to OXA treatment were made in East Africa [23]. The existence of OXA-R parasites was confirmed experimentally by infecting mice with parasites isolated from patients that were parasite positive following drug treatment [21–23]. Together these observations support the existence of segregating OXA-R alleles in schistosome populations before OXA deployment for parasite control.
Differences in OXA treatment efficacy between East and West Africa
Given the variation in OXA-R frequencies that we observe in different geographical samples, it is interesting to examine the literature on clinical treatment efficacy of OXA for treating schistosomiasis patients. Early trials of OXA to treat patients with intestinal schistosomiasis resulted in multiple treatment failures in Egypt, East Africa and South Africa [23–27]. As a consequence, the WHO recommended use of higher doses of OXA in East Africa, compared with West Africa [28]. Human host metabolism did not explain this lack of efficacy because the OXA availability in blood was the same or higher in the East African patient populations than in West African or South American populations [29,30]. Therefore, the presence of resistant parasites was the most likely explanation. OXA-R parasites were identified in Kenya by treating mice infected with parasites from patients showing poor treatment response [23]. We observed a 14.91% frequency of OXA-R alleles (and 5.26% frequency of homozygous OXA-R parasites) in East Africa compared with 4.29% frequency of OXA-R alleles (and no homozygous OXA-R parasites) in West Africa. Our results provide a molecular explanation for the poor treatment response observed in East Africa, relative to other locations.
Implications for development of OXA derivatives
OXA kills S. mansoni but not S. haematobium or S. japonicum. OXA derivatives are currently under development to obtain more potent molecules acting against all three schistosome species infecting humans. In fact, OXA derivatives that kill adult worms of the three species in vitro have now been developed [31]. The low frequency of homozygous OXA-R parasites in S. mansoni suggests that OXA derivatives will result in imperfect cure rate for this species, particularly in East Africa. However, the primary purpose of generating OXA derivatives is to treat S. haematobium and S. japonicum, perhaps as a partner drug for praziquantel (PZQ). Examining natural variation in homologous sulfotransferase genes from these two species (ShSULT-OR and SjSULT-OR) to better understand the potential for resistance evolution will be critical if OXA derivatives are to be developed to control these parasites.
Implications of standing variation for drug resistance evolution
Standing variation in drug resistance genes has a major impact on how fast resistance evolves [2]. If resistance alleles are already present in the population prior to treatment, drug resistance has the potential to spread much more rapidly because there is no waiting time for a resistance mutation to appear de novo (Fig 7A) [3]. An increasing number of examples support this view. The nematode Caenorhabditis elegans carries a large diversity of β-tubulin alleles allowing worms to be resistant to benzimidazoles (BZ) [32]. In the filarial worm Onchocerca volvulus, standing variation appears to be involved in resistance to ivermectin [6]. Standing variation is also involved in herbicide resistance in plants: resistance alleles from the weed Alopecurus myosuroides were identified in plant collection almost 100 years before herbicides were used [33]. Antibiotic resistance provides particularly dramatic examples of standing variation: plasmids encoding multidrug resistance were found in frozen bacteria isolated from the permafrost demonstrating that antibiotic resistance plasmids were already present thousands of years ago [34,35].
We do not want to give the impression that standing variation is the only source of variation for evolution of drug resitance. In the case of other well studied helminth parasites, such as Haemonchus contortus and Teladorsagia circumcincta, resistance alleles are consistent with evolution through recurrent de novo mutations or very rare standing variants. While selection of rare pre-existing BZ resistance alleles was shown in French farms [36], selection of de novo resistance mutations, associated with different haplotypes, was shown to be common in UK farms [37].
The fact that there is strong evidence of standing variation in OXA resistance but not in BZ resistance may reflect the differences in the nature of the mutation underlying resistance. In the case of OXA, resistance is due to loss-of-function mutations resulting in non functional SmSULT-OR. As a consequence, mutations can occur in any position that disrupts substrate binding or protein structure. Hence mutations are expected to arise frequently and will exist in natural populations. In contrast, mutations underlying BZ resistance occur in specific position within the β-tubulin gene. As consequence, such mutations occur much more rarely. Furthermore because β-tubulin is an essential gene, mutations that disrupt protein structure and result in non functional proteins are strongly selected against and cannot reach high frequency.
Standing variation for OXA resistance alleles could arise by drift, or alternatively as a result of selective forces other than OXA treatment. For example, Hahnel et al. [32] demonsrated BZ resistance in natural population of C. elegans which are not targetted by BZ drugs and speculated that natural compound with similar structure to benzimidazoles could have pre-selected for benzimidazole resistance alleles. In the case of our OXA-R mutations in SmSULT-OR, we do not know what selective forces might be involved (see also discussion in section Prediction of the resistance spread).
Drug resistance in parasitic helminths infecting livestock or humans is now recognized as a potential concern for control efforts, but was neglected for a long time using the argument that the slow reproduction rate of worms would greatly delay resistance evolution [38]. The observation that resistance alleles exist as standing variation within multiple pathogen or parasite populations in the absence of drug treatment suggests previous optimism about the shelf life of drugs was poorly founded. If resistance alleles are already at relatively high frequency in populations when new drugs are deployed, resistance may evolve extremely rapidly [3,5].
Prediction of the resistance spread
We can use the frequency of resistance allele to model resistance spread in parasite populations. In the case of OXA resistance, using an Ne estimate of 65,000 [39], OXA-R alleles spread from 15% starting frequency to fixation in 170 generations in 100% of simulations when selection is strong (s = 0.1). Assuming a generation time between 3 months and 1 year, this is equivalent to a period of 42.5 to 170 years (Fig 7A). However, if OXA-R alleles were evolving de novo, simulations indicate that fixation will occur rarely (0.04% of the simulations) and this would take 1,197 generations (corresponding to a period of 299.25 to 1,197 years) from the time a mutation arises. We can use the observed frequency of OXA-R alleles segregating in East African populations to make predictions about the spread of phenotypically resistant parasites (i.e. those homozygous for OXA-R alleles) (Fig 7B). If we use a frequency of 10% resistant parasites to denote unacceptable efficacy (this threshold is used for antimalarial drugs [40]) then this threshold will be reached in 19 to 444 generations (4.75 to 444 years) using a range of selection coefficients (from 0.01 to 0.2). The selection coefficients driving spread of drug resistance alleles in schistosomes is not known, but the range we have used covers measured selection coefficients driving drug resistance spread in another human parasite (Plasmodium falciparum) [41,42]. These simulations suggest that, while OXA-R allele frequencies are relatively high in East Africa, OXA derivatives could still be extremely effective for schistosome control in the short-medium term, particularly if deployed as a combination therapy, partnered with a second drug with a different mode of action (e.g. PZQ).
Our simple models for predicting spread of OXA-R alleles under drug selection ignore fitness costs of OXA-R alleles, which might limit the rate of spread. The fitness cost of inactive SmSULT-OR in the absence of drug treatment still needs to be assessed accurately [13] but the occurrence of natural homozygotes for defective alleles suggests limited costs. The exact role of this enzyme in schistosome biology is not known. Sulfotransferases transfer the sulfo group from a donor (usually PAPS) to the substrate, resulting in inactivation or solubilization of the substrate [43]. Substrates can be endogenous signal molecules (hormones, neurotransmitters) or exogenous chemicals. In the latter case, sulfotransferases play a role in detoxifying natural or synthetic toxins [43], but sometimes they do the opposite by activating pro-drugs as observed with the anti-tumor drug N-benzyl indolecarbinols [44] or OXA [10,45]. Because the same enzyme can sulfate both endogenous and exogenous compounds, it is often difficult to determine their exact biological role. In schistosomes, we speculate that SmSULT-OR could have roles in regulating endogenous molecules as well as detoxifying chemicals present in their environment (i.e. in the feces, water, snail or human blood, etc.).
Molecular markers for resistance surveillance
Molecular markers of OXA-R now allow efficient monitoring of the distribution of OXA-R alleles in schistosome populations. This approach is widely used for other parasites and pathogens such as malaria parasite [46] and HIV [47]. For schistosome, collections such as SCAN which contain thousands of parasite samples from multiple locations [17] will be extremely valuable for this work: regular sampling in different endemic regions will help to update the resistance landscape. However, not all identified SmSULT-OR mutations will lead to resistance, so it is critical that molecular screening is paired with functional evaluation or computational prediction, as we have done here.
PZQ is the only drug currently available to treat schistosomiasis and effort in mass drug administration has recently been expanded 10 fold [48], with a target of administering 250 million treatments per year, increasing selective pressure for PZQ resistance. We suspect that standing variation for PZQ resistance is also likely, because this trait is easy to select in the laboratory. This has been done by several groups using independent laboratory populations, and resistance has spread within very few generations [49–54], strongly suggesting that resistance alleles were already present in these laboratory populations. Furthermore, the laboratory populations used for selection were isolated before PZQ was available [23,55] and so were not exposed to drug selection. Molecular markers for monitoring the frequency of PZQ-R in natural parasite populations would be extremely valuable, as demonstrated by our work on OXA-R.
Materials and methods
Ethics statement
African samples
Samples from Senegal and Niger were collected as part of the EU-CONTRAST project [56], a multidisciplinary alliance to optimize schistosomiasis control and transmission surveillance in sub-Saharan Africa, as detailed in Webster et al. [57] with ethical approval granted by ethical committees of the Ministry of Health (Dakar, Senegal), and the Niger National Ethical Committee (Niamey, Niger) with additional ethical approval obtained from the St Mary’s Hospital Local Ethics Research Committee, R&D office (part of the Imperial College Research Ethics Committee (ICREC; EC no. 03.36. R&D no. 03/SB/033E)), in combination with the ongoing CONTRAST and Schistosomiasis Control Initiative (SCI) activities.
For the Tanzanian samples collected as part of the Schistosomiasis Consortium for Operational Research and Evaluation (SCORE), ethical approvals were granted by the Imperial College Research Ethics Committee and the SCI Ethical approval (EC no. 03.36. R&D no. 03/SB/033E); the National Institute for Medical Research (NIMR, reference no. NIMR/HQ/R.8a/Vol. IX/1022); University of Georgia Institutional Review Boards, Athens, GA (2011-10353-1).
Following routine procedures in the field, the objectives of the study were first explained to the local village chiefs and political and religious authorities who gave their consent to conduct the study. Written consent for the schoolchildren to participate in longitudinal monitoring of the national control programme for schistosomiasis was given by head teachers, and/or village chiefs where there were no schools, due to the fact that in African villages, written consent of the child’s guardian is often very difficult to obtain owing to the associated impoverished conditions and often low literacy. Each individual child also gave verbal consent before recruitment. Following sampling, a praziquantel treatment (40 mg.kg−1) was offered to infected participants.
Omani samples
We obtained ethical clearance from the Sultan Qaboos University and the Ministry of Health of Oman to use positive stool samples for schistosomiasis collected by the Ministry of Health of Oman during epidemiological screening. Ethical approval was given by the Medical Research and Ethical Committee (MREC) of the Non-Communicable Disease Control Section of the Directorate General of Health Affairs, Headquarters, Ministry of Health, Oman (no. MH/DGHA/DSDC/NCD/R&S/167/01).
We obtained ethical approvals of animal studies in France from the French Ministère de l’Éducation Nationale, de la Recherche et de la Technologie, from the French Ministère de l’Agriculture et de la Pêche (agreement no. A 66040), and from the French Direction Départementale de la Protection des Populations (no. C 66-136-01 with prefectoral order no. 2012-201-0008). Certificate for animal experimentation was given to H.M. (authorization no. C 66.11.01; articles no. R 214–87, R 214–122 and R 215–10). Housing, breeding and animal care followed the guidelines of the French CNRS. The different protocols used in this study had been approved by the French veterinary agency from the DRAAF Languedoc-Roussillon (Direction Régionale de l'Alimentation, de l'Agriculture et de la Forêt), Montpellier, France (authorization no. 007083).
Sampling
African samples
S. mansoni miracidia were collected from individual patients in two different West African countries in 2007: 27 patients from two locations in Senegal, and 17 patients from two locations in Niger (S3 Table) [57,58]. Additionally, S. mansoni miracidia were collected as part of the SCORE program from 64 children in seven villages on the shores of Lake Victoria in Tanzania (East Africa) in January 2012 (S3 Table) [59].
Collection of miracidia was performed as previously described [57,60]. Briefly, individual stool samples from positive patients were homogenized through a mesh and washed through with water. The content was then transferred in a Pitchford funnel assembly, washed with additional water, and the filtered homogenate was drained into a Petri dish. The Petri dish containing the homogenate was then left in bright ambient light (not direct sunlight) to allow hatching of miracidia. Miracidia were visualized under a dissecting microscope and individually captured in 3–5 μL of water using a micropipette. Miracidia were then pipetted individually onto Whatman FTA cards for DNA preservation. Cards were allowed to dry for 1 hour and then stored for future research [57,58,60].
Omani samples
The Omani schistosome samples were collected during 8 field trips, from 2001 to 2015, in different areas in Dhofar, Oman and originated from either Homo sapiens, Rattus rattus or Biomphalaria pfeifferi (S3 Table): 11 patients from 4 localities, 2 rats from 2 localities, 26 snails from 3 localities.
We obtained schistosome adult worms from naturally infected rats and from laboratory infected mice. The mice were infected with cercariae from naturally infected snails or from laboratory snails infected with miracidia isolated from stool samples. We collected miracidia from stool samples as described in Moné et al. [61]. We exposed Biomphalaria pfeifferi snails to miracidia as described in Mouahid et al. [62]. We maintained laboratory and naturally infected snails at constant temperature (26°C) and balanced photoperiod (12h light / 12h dark) and fed them with fresh lettuce ad libitum. Prior to infection, we anaesthetized the mice by intraperitoneal injection of an anesthetic solution (0.01 mL.g-1 of mouse body weight). We prepared the anaesthetic solution using 0.5 mL of Rompun (20 mg.mL-1; Bayer) and 1 mL of Imalgène (100 mg.mL-1; Rhône Mérieux) diluted in 8.5 mL of autoclaved NaCl 8.5 ‰. Abdomens of anesthetized mice were shaved and exposed during 1 hour to cercariae shed from laboratory or naturally infected snails. Cercariae from naturally infected snails were kept in 95° ethanol when the number of cercariae was too low for mouse infection. Laboratory infected mice or naturally infected rats were euthanized by intraperitoneal injection of sodium pentobarbital solution (1.1 mL diluted in 10 mL of ethanol 10%). We recovered adult worms by perfusion [63]; the worms were washed in NaCl 8.5 ‰, and single worms (female or male) were fixed in 95° ethanol and preserved at -20°C. Worms and cercariae were washed in 1X TE buffer for 1 hour prior to DNA extraction.
DNA processing and library preparation
We amplified DNA and sequenced exomes from single miracidia preserved on FTA cards (1 miracidium per patient) for African samples or from DNA extracted from worms or cercariae for Omani samples. FTA-preserved samples were processed following our published protocol [64]. Briefly we punched a 2 mm disc containing the miracidium from the FTA card, washed it with the FTA Purification Reagent (GE Healthcare Life Sciences), rinsed it twice with TE−1 buffer, and finally dried it. DNA from single worms and cercariae were extracted using the DNeasy Blood and Tissue kit (Qiagen) following the tissue protocol with an incubation time of 2h at 56°C and an elution in 200 μL. Samples were quantified using the Qubit dsDNA HS assay kit (Invitrogen).
We then performed whole genome amplification (WGA) on each FTA punch or on 2 μL or 4 μL of DNA solution using the Illustra GenomiPhi V2 DNA Amplification kit (GE Healthcare Life Sciences). Amplified DNA was purified with the SigmaSpinTM Sequencing reaction Clean-up (Sigma-Aldrich), following the manufacturer protocol. We quantified purified samples using the Qubit dsDNA BR assay (Invitrogen). DNA samples that failed WGA were cleaned up and concentrated using the Genomic DNA Clean & Concentrator kit (Zymo Research) following the manufacturer protocol and these samples with concentrated DNA were used to perform a new WGA.
Because WGA reactions are non-specific, we performed qPCR on each sample from FTA cards to quantify proportion of schistosome DNA (i.e., check for an excess of amplified DNA from the environment (water, fecal matter, etc.)). We excluded samples that showed less than 100 schistosome genomes copies in 20 ng of DNA which were not suitable for subsequent exome capture. We assessed genome copies by quantifyng the S. mansoni α-tubulin single copy gene [64].
We prepared exome capture libraries on the selected African samples and on all the successfully amplified Omani samples using the pre-capture pooling method of the SureSelect XT2 Target Enrichment System (Agilent) [64]. Finally, we sequenced libraries on a HiSeq 2500 and data were demultiplexed using the Casava pipeline. Raw sequencing data are accessible from the NCBI Sequence Read Archive under BioProject accession numbers PRJNA439266, PRJNA560069, PRJNA560070.
Alignment, variant calling and phasing
We aligned sequences as described in Le Clec’h et al. [64]. Briefly, we aligned data against the v5 S. mansoni genome using BWA and SAMtools, realigned around indels using GATK, PCR duplicates were marked using picard and Q-score were recalibrated using GATK.
To identify variants in the SmSULT-OR (Smp_089320) gene, we performed a variant calling using FreeBayes (v1.1.0-46-g8d2b3a0) [65] in the first 3 Mb of the chromosome 6. We used a minimum base quality (-q) of 20 and a minimum mapping quality (-m) of 30. We included mutation sites identified previously [10,13] in order to genotype these sites specifically for further comparison. We defined 4 populations: Caribbean (HR), West Africa (SN and NE), East Africa (TZ) and Middle-East (OM). We excluded variants supported by less than 4 reads using VCFtools (v0.1.14) [66]. We then used vcf-subset from VCFtools to remove sites with reference allele only. We adjusted variant positions using vt normalize (v0.5772-60f436c3) [67]. We finally used Beagle (v4.1) [68] to first estimate genotypes and to then phase data. This approach resolves haplotypes using statistical models rather than by direct linkage of SNPs on short reads. Unphased variant calling data and scripts used to analyze the data are available on Zenodo (DOI: 10.5281/zenodo.2850876 and 10.5281/zenodo.3370039, respectively).
Genetics analysis
We performed all the genetics analysis using phased exome data. We handled the VCF data in R (R v3.5.0 [69]) using the vcfR package (v1.8.0.9) [70]. We determined the longest haplotype block from the samples carrying the p.E142del using a custom R code after filtering invariable sites in these samples. We built the haplotype network using the R package poppr (v2.8.1) [71,72] after selecting variants within the longest haplotype coordinates showing no more than 20% of missing data. We computed the frequency of the OXA-R allele using a custom R script and functional annotation obtained through a custom bash script.
We generated haplotype sequences of the coding sequence only for the Old World samples using the updated phased VCF data exported from R and the BCFtools (v1.2) [73]. We generated haplotypes of the South American samples from previous genotyping using a custom bash script. We included the SmSULT-OR homologue sequence from S. rodhaini previously identified [13]. We aligned the haplotypes using Clustal Omega (v1.2.2) [74]. We determined nucleotide diversity, number of synonymous and non-synonymous sites using DnaSP software (v6.12.01) [75]. Populations were defined as described in the previous section. Scripts used to analyze the data are available on Zenodo (DOI: 10.5281/zenodo.3370039).
Recombinant SmSULT-OR protein production and OXA activation assay
We produced recombinant SmSULT-OR proteins following Chevalier et al. [13]. Briefly, mutations were introduced in the cloned SmSULT-OR gene sequence (Smp_089320; GenBank accession no. HE601629.1) and introduced into Escherichia coli for protein production. Proteins were extracted from bacterial culture and purified on an affinity chromatography column. His-tag was removed using Tobacco etch virus (TEV) protease, the solution was dialyzed overnight and passed through affinity chromatography again to remove His-tag and TEV protease. The sample was loaded onto a GE-pre-packed Q anion exchange column and eluted, and the pooled fractions were dialyzed overnight. The protein was finally concentrated at 10 mg.mL-1.
We tested the impact of a subset of the mutations observed on OXA activation using an in vitro assay [13]. We tested the ability of recombinant SmSULT-OR proteins to activate OXA in a protease inhibitor cocktail with sheared S. mansoni gDNA as a final target for the tritiated OXA pre-mixed with ATP, MgCl2 and PAPS co-factor. After 2.5 h of incubation at 37°C, the reaction was stopped and DNA was extracted three times. Radioactivity in the remaining aqueous phase containing the DNA and in water (blank) was counted in a liquid scintillation spectrometer. Blank values were subtracted from sample values. We performed three independent reactions for each recombinant protein.
Evaluation of impact of mutations on protein stability
We first visually assessed the scored mutations on the SmSULT-OR protein structure (PDB code 4MUB [10]) using the mutagenesis function of PyMol software (v2.3.0; Schrödinger, LLC).
We then used the Rosetta package (v3.9) to test the impact of scored mutations on protein stability. We used the ddg_monomer application to compute the difference in free enthalpy (ΔΔG) between the mutated (amino acid substitutions only) and the wild-type protein. The higher the ΔΔG, the more unstable is the mutated protein. For this, we prepared ligand files (OAQ and A3P) by downloading SDF files containing all ligand structures from SmSULT-OR crystals available from the Protein Data Bank website. We modified SDF files to add hydrogens using Openbabel (v2.4.0) [76], and generated params files using molfile_to_params.py from Rosetta. We used the structure of SmSULT-OR to generate a constraint file using the minimize_with_cst application and the convert_to_cst_file.sh scripts. We removed ligand information from the constraint file. For each mutation, we finally generated a resfile and ran the ddg_monomer application with 50 iterations and options adapted from the protocol 16 of Kellogg et al. [77]. Scripts used to run this analysis are available on Zenodo (DOI: 10.5281/zenodo.3370039).
Statistical analysis
We conducted statistical analyses using R (v3.5.0) [69]. We performed simulations using a modified version of the driftR simulator [78]. The OXA activation assay data were tested for normality using a Shapiro-Wilk and compared by a parametric ANOVA followed by a Tukey post-hoc test.
Supporting information
S1 Table [xlsx]
Library sequencing statistics.
S2 Table [xlsx]
Mutations scored in samples from West Africa (Senegal and Niger), East Africa (Tanzania) and Middle East (Oman).
S3 Table [xlsx]
Sampling information for the schistosome material used.
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Štítky
Hygiena a epidemiologie Infekční lékařství LaboratořČlánek vyšel v časopise
PLOS Pathogens
2019 Číslo 10
- Farmakovigilanční studie perorálních antivirotik indikovaných v léčbě COVID-19
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