Pax6 organizes the anterior eye segment by guiding two distinct neural crest waves
Autoři:
Masanari Takamiya aff001; Johannes Stegmaier aff002; Andrei Yu Kobitski aff001; Benjamin Schott aff002; Benjamin D. Weger aff001; Dimitra Margariti aff001; Angel R. Cereceda Delgado aff001; Victor Gourain aff001; Tim Scherr aff002; Lixin Yang aff001; Sebastian Sorge aff001; Jens C. Otte aff001; Volker Hartmann aff004; Jos van Wezel aff005; Rainer Stotzka aff004; Thomas Reinhard aff006; Günther Schlunck aff006; Thomas Dickmeis aff001; Sepand Rastegar aff001; Ralf Mikut aff002; Gerd Ulrich Nienhaus aff001; Uwe Strähle aff001
Působiště autorů:
Institute of Biological and Chemical Systems - Biological Information Processing, Karlsruhe Institute of Technology, Karlsruhe, Germany
aff001; Institute for Biological and Chemical Systems - Biological Information Processing, Karlsruhe Institute of Technology, Karlsruhe, Germany
aff001; Institute for Automation and Applied Informatics, Karlsruhe Institute of Technology, Karlsruhe, Germany
aff002; Institute of Applied Physics, Karlsruhe Institute of Technology, Karlsruhe, Germany
aff003; Institute for Data Processing and Electronics, Karlsruhe Institute of Technology, Karlsruhe, Germany
aff004; Steinbuch Centre for Computing, Karlsruhe Institute of Technology, Karlsruhe, Germany
aff005; Eye Center, Freiburg University Medical Center, Freiburg, Germany
aff006; Institute of Nanotechnology, Karlsruhe Institute of Technology, Karlsruhe, Germany
aff007; Department of Physics, University of Illinois at Urbana-Champaign, Urbana, Illinois, United States of America
aff008
Vyšlo v časopise:
Pax6 organizes the anterior eye segment by guiding two distinct neural crest waves. PLoS Genet 16(6): e32767. doi:10.1371/journal.pgen.1008774
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pgen.1008774
Souhrn
Cranial neural crest (NC) contributes to the developing vertebrate eye. By multidimensional, quantitative imaging, we traced the origin of the ocular NC cells to two distinct NC populations that differ in the maintenance of sox10 expression, Wnt signalling, origin, route, mode and destination of migration. The first NC population migrates to the proximal and the second NC cell group populates the distal (anterior) part of the eye. By analysing zebrafish pax6a/b compound mutants presenting anterior segment dysgenesis, we demonstrate that Pax6a/b guide the two NC populations to distinct proximodistal locations. We further provide evidence that the lens whose formation is pax6a/b-dependent and lens-derived TGFβ signals contribute to the building of the anterior segment. Taken together, our results reveal multiple roles of Pax6a/b in the control of NC cells during development of the anterior segment.
Klíčová slova:
Cell migration – Cornea – Embryos – Endothelium – Eye lens – Eyes – Zebrafish – Diencephalon
Zdroje
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