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Suppression of class I compensated cell enlargement by xs2 mutation is mediated by salicylic acid signaling


Autoři: Fujikura Ushio aff001;  Kazune Ezaki aff002;  Gorou Horiguchi aff003;  Mitsunori Seo aff004;  Yuri Kanno aff004;  Yuji Kamiya aff004;  Michael Lenhard aff005;  Hirokazu Tsukaya aff002;  Ushio Fujikura aff001
Působiště autorů: Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Japan aff001;  Graduate School of Science, The University of Tokyo, Japan aff002;  Department of Life Science, College of Science, Rikkyo University, Japan aff003;  RIKEN Center for Sustainable Resource Science, Japan aff004;  Institute for Biochemistry and Biology, University of Potsdam, Germany aff005;  Institut für Biochemie und Biologie, Universität Potsdam, Potsdam-Golm, Germany aff005;  Okazaki Institute for Integrative Bioscience, Japan aff006
Vyšlo v časopise: Suppression of class I compensated cell enlargement by xs2 mutation is mediated by salicylic acid signaling. PLoS Genet 16(6): e32767. doi:10.1371/journal.pgen.1008873
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008873

Souhrn

The regulation of leaf size has been studied for decades. Enhancement of post-mitotic cell expansion triggered by impaired cell proliferation in Arabidopsis is an important process for leaf size regulation, and is known as compensation. This suggests a key interaction between cell proliferation and cell expansion during leaf development. Several studies have highlighted the impact of this integration mechanism on leaf size determination; however, the molecular basis of compensation remains largely unknown. Previously, we identified extra-small sisters (xs) mutants which can suppress compensated cell enlargement (CCE) via a specific defect in cell expansion within the compensation-exhibiting mutant, angustifolia3 (an3). Here we revealed that one of the xs mutants, namely xs2, can suppress CCE not only in an3 but also in other compensation-exhibiting mutants erecta (er) and fugu2. Molecular cloning of XS2 identified a deleterious mutation in CATION CALCIUM EXCHANGER 4 (CCX4). Phytohormone measurement and expression analysis revealed that xs2 shows hyper activation of the salicylic acid (SA) response pathway, where activation of SA response can suppress CCE in compensation mutants. All together, these results highlight the regulatory connection which coordinates compensation and SA response.

Klíčová slova:

Arabidopsis thaliana – Cell proliferation – Gene expression – Leaf development – Leaves – Phenotypes – Plant hormones – Salicylic acid


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