Germ cell-intrinsic effects of sex chromosomes on early oocyte differentiation in mice

English version

Autoři: Norio Hamada ^aff001; Nobuhiko Hamazaki ^aff001; So Shimamoto ^aff001; Orie Hikabe ^aff001; Go Nagamatsu ^aff001; Yuki Takada ^aff001; Kiyoko Kato ^aff002; Katsuhiko Hayashi ^aff001
Působiště autorů: Department of Stem Cell Biology and Medicine, Graduate School of Medical Sciences, Kyushu University, Maidashi, Higashi-ku, Fukuoka, Japan ^aff001; Department of Obstetrics and Gynecology, Graduate School of Medical Sciences, Kyushu University, Maidashi, Higashi-ku, Fukuoka, Japan ^aff002
Vyšlo v časopise: Germ cell-intrinsic effects of sex chromosomes on early oocyte differentiation in mice. PLoS Genet 16(3): e32767. doi:10.1371/journal.pgen.1008676
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008676

Souhrn

A set of sex chromosomes is required for gametogenesis in both males and females, as represented by sex chromosome disorders causing agametic phenotypes. Although studies using model animals have investigated the functional requirement of sex chromosomes, involvement of these chromosomes in gametogenesis remains elusive. Here, we elicit a germ cell-intrinsic effect of sex chromosomes on oogenesis, using a novel culture system in which oocytes were induced from embryonic stem cells (ESCs) harboring XX, XO or XY. In the culture system, oogenesis using XO and XY ESCs was severely disturbed, with XY ESCs being more strongly affected. The culture system revealed multiple defects in the oogenesis of XO and XY ESCs, such as delayed meiotic entry and progression, and mispairing of the homologous chromosomes. Interestingly, Eif2s3y, a Y-linked gene that promotes proliferation of spermatogonia, had an inhibitory effect on oogenesis. This led us to the concept that male and female gametogenesis appear to be in mutual conflict at an early stage. This study provides a deeper understanding of oogenesis under a sex-reversal condition.

Klíčová slova:

Cell differentiation – Gene expression – Germ cells – Homologous chromosomes – Oocytes – Oogenesis – Sex chromosomes – X chromosomes

Zdroje

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