A kinesin Klp10A mediates cell cycle-dependent shuttling of Piwi between nucleus and nuage

English version

Autoři: Zsolt G. Venkei ^aff001; Charlotte Choi ^aff002; Suhua Feng ^aff003; Cuie Chen ^aff001; Steven E. Jacobsen ^aff003; John K. Kim ^aff002; Yukiko M. Yamashita ^aff001; Charlotte P. Choi ^aff002
Působiště autorů: Life Sciences Institute, University of Michigan, Ann Arbor, Michigan, United States of America ^aff001; Department of Biology, Johns Hopkins University, Baltimore, Maryland, United States of America ^aff002; Department of Molecular, Cell and Developmental Biology, University of California, Los Angeles, California, United States of America ^aff003; Eli and Edyth Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles, California, United States of America ^aff004; Howard Hughes Medical Institute, University of California, Los Angeles, California, United States of America ^aff005; Department of Cell and Developmental Biology, University of Michigan, Ann Arbor, Michigan, United States of America ^aff006; Howard Hughes Medical Institute, University of Michigan Ann Arbor, Michigan, United States of America ^aff007
Vyšlo v časopise: A kinesin Klp10A mediates cell cycle-dependent shuttling of Piwi between nucleus and nuage. PLoS Genet 16(3): e32767. doi:10.1371/journal.pgen.1008648
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008648

Souhrn

The piRNA pathway protects germline genomes from selfish genetic elements (e.g. transposons) through their transcript cleavage in the cytoplasm and/or their transcriptional silencing in the nucleus. Here, we describe a mechanism by which the nuclear and cytoplasmic arms of the piRNA pathway are linked. We find that during mitosis of Drosophila spermatogonia, nuclear Piwi interacts with nuage, the compartment that mediates the cytoplasmic arm of the piRNA pathway. At the end of mitosis, Piwi leaves nuage to return to the nucleus. Dissociation of Piwi from nuage occurs at the depolymerizing microtubules of the central spindle, mediated by a microtubule-depolymerizing kinesin, Klp10A. Depletion of klp10A delays the return of Piwi to the nucleus and affects piRNA production, suggesting the role of nuclear-cytoplasmic communication in piRNA biogenesis. We propose that cell cycle-dependent communication between the nuclear and cytoplasmic arms of the piRNA pathway may play a previously unappreciated role in piRNA regulation.

Klíčová slova:

Cell cycle and cell division – Drosophila melanogaster – Germ cells – Mitosis – MTS assay – RNA sequencing – Testes – Transposable elements

Zdroje

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