pH-dependent activation of cytokinesis modulates Escherichia coli cell size

English version

Autoři: Elizabeth A. Mueller ^aff001; Corey S. Westfall ^aff001; Petra Anne Levin ^aff001
Působiště autorů: Department of Biology, Washington University in St. Louis, St. Louis, Missouri, United States of America ^aff001
Vyšlo v časopise: pH-dependent activation of cytokinesis modulates Escherichia coli cell size. PLoS Genet 16(3): e32767. doi:10.1371/journal.pgen.1008685
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008685

Souhrn

Cell size is a complex trait, derived from both genetic and environmental factors. Environmental determinants of bacterial cell size identified to date primarily target assembly of cytosolic components of the cell division machinery. Whether certain environmental cues also impact cell size through changes in the assembly or activity of extracytoplasmic division proteins remains an open question. Here, we identify extracellular pH as a modulator of cell division and a significant determinant of cell size across evolutionarily distant bacterial species. In the Gram-negative model organism Escherichia coli, our data indicate environmental pH impacts the length at which cells divide by altering the ability of the terminal cell division protein FtsN to localize to the cytokinetic ring where it activates division. Acidic environments lead to enrichment of FtsN at the septum and activation of division at a reduced cell length. Alkaline pH inhibits FtsN localization and suppresses division activation. Altogether, our work reveals a previously unappreciated role for pH in bacterial cell size control.

Klíčová slova:

Bacterial evolution – Cell cycle and cell division – Cell walls – Cytokinesis – Glucose – Hyperexpression techniques – Periplasm – Phase contrast microscopy

Zdroje

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Článek The Caenorhabditis elegans homolog of the Evi1 proto-oncogene, egl-43, coordinates G1 cell cycle arrest with pro-invasive gene expression during anchor cell invasion

Článek Transcription-replication conflicts as a source of common fragile site instability caused by BMI1-RNF2 deficiency

Článek The Lid/KDM5 histone demethylase complex activates a critical effector of the oocyte-to-zygote transition

Článek Tracking human population structure through time from whole genome sequences

Článek FLS2 is a CDK-like kinase that directly binds IFT70 and is required for proper ciliary disassembly in Chlamydomonas

Článek Cell cycle transcriptomics of Capsaspora provides insights into the evolution of cyclin-CDK machinery