The Effects of Somatic Hypermutation on Neutralization and Binding in the PGT121 Family of Broadly Neutralizing HIV Antibodies
Broadly neutralizing HIV antibodies (bnAbs) are typically highly somatically mutated, raising doubts as to whether they can be elicited by vaccination. We used 454 sequencing and designed a novel phylogenetic method to model lineage evolution of the bnAbs PGT121–134 and found a positive correlation between the level of somatic hypermutation (SHM) and the development of neutralization breadth and potency. Strikingly, putative intermediates were characterized that show approximately half the mutation level of PGT121–134 but were still capable of neutralizing roughly 40–80% of PGT121–134 sensitive viruses in a 74-virus panel at median titers between 15- and 3-fold higher than PGT121–134. Such antibodies with lower levels of SHM may be more amenable to elicitation through vaccination while still providing noteworthy coverage. Binding characterization indicated a preference of inferred intermediates for native Env binding over monomeric gp120, suggesting that the PGT121–134 lineage may have been selected for binding to native Env at some point during maturation. Analysis of glycan-dependent neutralization for inferred intermediates identified additional adjacent glycans that comprise the epitope and suggests changes in glycan dependency or recognition over the course of affinity maturation for this lineage. Finally, patterns of neutralization of inferred bnAb intermediates suggest hypotheses as to how SHM may lead to potent and broad HIV neutralization and provide important clues for immunogen design.
Published in the journal:
. PLoS Pathog 9(11): e32767. doi:10.1371/journal.ppat.1003754
Category:
Research Article
doi:
https://doi.org/10.1371/journal.ppat.1003754
Summary
Broadly neutralizing HIV antibodies (bnAbs) are typically highly somatically mutated, raising doubts as to whether they can be elicited by vaccination. We used 454 sequencing and designed a novel phylogenetic method to model lineage evolution of the bnAbs PGT121–134 and found a positive correlation between the level of somatic hypermutation (SHM) and the development of neutralization breadth and potency. Strikingly, putative intermediates were characterized that show approximately half the mutation level of PGT121–134 but were still capable of neutralizing roughly 40–80% of PGT121–134 sensitive viruses in a 74-virus panel at median titers between 15- and 3-fold higher than PGT121–134. Such antibodies with lower levels of SHM may be more amenable to elicitation through vaccination while still providing noteworthy coverage. Binding characterization indicated a preference of inferred intermediates for native Env binding over monomeric gp120, suggesting that the PGT121–134 lineage may have been selected for binding to native Env at some point during maturation. Analysis of glycan-dependent neutralization for inferred intermediates identified additional adjacent glycans that comprise the epitope and suggests changes in glycan dependency or recognition over the course of affinity maturation for this lineage. Finally, patterns of neutralization of inferred bnAb intermediates suggest hypotheses as to how SHM may lead to potent and broad HIV neutralization and provide important clues for immunogen design.
Introduction
A successful vaccine against HIV will likely require the elicitation of antibody responses capable of neutralizing a majority of global isolates. Recent work has suggested that 5–20% of HIV chronically-infected individuals naturally develop broadly neutralizing responses to some degree, but how these responses emerge and mature are unclear [1]–[8]. A common observation among bnAbs is their unusually high level of somatic hypermutation (SHM), which on average constitutes around 20% divergence (range: 7–32%) from the putative germline nucleotide sequence (nt) for the variable heavy chain (VHJH) region (DH-genes were left out of these analyses because of ambiguity associated with D-gene assignment) [2]–[7], [9]. For example, the CD4 binding site bnAb VRC01 is 30% and 19% mutated in its variable heavy (VHJH) and light (VLJL) chain sequence, respectively [4], [6]. The V2 quaternary epitope-specific bnAbs PG9 and PGT145 are relatively less mutated with 14–19% mutation frequency in VHJH and 11–17% in VLJL, but both have unusually long CDRH3s of 30–33 amino acids [2], [5]. Finally, the recently described PGT121, 128 and 135 antibodies, which bind to protein-glycan epitopes in the variable V3 and V4 regions and demonstrate the highest potency yet observed against a broad panel of HIV isolates, are 17–23% divergent in VHJH and 11–28% divergent in VLJL [5], [8], [10]. Of note, a number of the bnAbs also have insertions or deletions (indels) in their variable regions and recent crystal structures have identified indels as critical for protein or glycan contacts on HIV Env [4], [7], [11]. Interestingly, gp120-reactive antibodies that show no or low neutralizing activity from chronically HIV-infected individuals demonstrate a relatively high but lesser degree of SHM than bnAbs, in the range of 9–12% in VHJH [12], [13].
In contrast to HIV bnAbs, antibodies from vaccination in general have an average nt mutation frequency of 6% (range: 1–30%) in the VH, which has cast doubts on the likelihood of eliciting bnAbs through vaccination [14]–[21]. We note that while these previous studies are caveated by insufficient sampling of antibody responses, due in large part to technological limitations, they nonetheless provide an approximation of the large discrepancy in mutation frequency between antibodies typically elicited through vaccination and HIV bnAbs. Currently, no immunogen has reliably elicited significant levels of HIV bnAbs to circulating viruses. Part of this failure might be due to inadequate immunogen design, but assuming high mutation levels play a significant role in enabling neutralizing breadth and potency, it is also possible that current immunization approaches are failing because of an inability to elicit sufficient levels of SHM. In one report, immunization with monomeric gp120 in humans was only capable of eliciting antibodies with an average mutation frequency of ∼6% [18]. Similarly, Env trimer immunization in rhesus macaques produced an average VH mutation frequency of 10% [19]. A further complexity is the association of broad neutralization with indels, sometimes relatively long, in some bnAbs as described above. The frequency of indels is associated with the extent of SHM; typical immunization procedures that produce moderate levels of SHM produce a relatively low number of indels and the frequency of longer indels is particularly uncommon. The frequency of indels among bnAbs may therefore present another obstacle for their elicitation by vaccination [22].
Given the large gap between the average mutation frequency of antibodies elicited by vaccination and bnAbs, it will prove helpful to identify broad and potent neutralizing antibodies with lower mutation frequencies as viable candidates for bnAb elicitation by vaccination. The levels of SHM that are necessary for broad and potent neutralization is poorly defined. Much work has been done to demonstrate that complete reversion of VHDHJH and VLJL sequences of HIV bnAbs to the corresponding germline sequences generally abrogates neutralizing and binding activity, which suggests that some degree of SHM plays an important role in the development of neutralizing breadth and potency [23]–[26]. More recent studies involving reversion of all framework (FR) mutations was shown to affect binding and neutralization for bnAbs but not for non-broadly neutralizing antibodies, suggesting that high levels of SHM are necessary for neutralization breadth and potency [9]. Similarly, a 12 amino acid reversion in the VH of VRC01 was sufficient to negatively affect neutralization breadth [4]. In a different study, characterization of inferred intermediates from longitudinal samples identified less-mutated CD4bs bnAb intermediates, though these putative precursors were greatly limited in neutralization breadth and potency [27]. Based on longitudinal serum neutralization data, it appears that neutralization breadth generally requires up to 4 years post-infection in order to develop, further supporting the idea that antibodies are going though many rounds of maturation before gaining breadth of neutralization [8], [10]. Longitudinal analysis of a SHIV-infected macaque, however, demonstrated the rapid emergence of neutralization breadth and potency against the N332 epitope suggesting potential differences in the dependence on SHM levels [8], [28], [29].
Previous work has attempted to map the maturation process from germline to affinity matured antibody for a group of highly broad and potent CD4 binding-site antibodies using deep sequencing analysis [6]. However, less mutated antibody intermediates identified by traditional phylogeny in that study did not demonstrate neutralization activity. To identify less-mutated intermediates with broad and potent neutralization, we used deep sequencing to study the development of neutralization breadth in the donor from whom bnAbs PGT121–123 were isolated. These antibodies are among the most potent bnAbs described to date and provide protection in macaques at relatively low serum levels [30]. Thus, the PGT121–123 family of antibodies are promising vaccine leads because their high potency suggests that even modest titers of antibody in the serum might be capable of contributing to protection. Ideally, the evolution of a bnAb response would be followed in longitudinal samples as infection progressed. However, since such samples were not available for this donor, we used 454 pyrosequencing to probe the memory B cell antibody repertoire and model the lineage evolution of the PGT121–123 family. We performed this analysis on PBMCs drawn at the same time point as that previously used to isolate PGT121–123 [5]. To analyze the resulting set of sequences, we developed a novel method called ImmuniTree, which is an alternative approach to conventional phylogenetic analyses and is designed specifically to model antibody SHM. Our results suggest that the PGT121 family of antibodies is capable of demonstrating appreciable breadth and high potency at relatively modest levels of SHM.
Results
ImmuniTree models SHM of the PGT121–123 lineage
To produce libraries for 454 sequencing, gene-specific primers were used on 54,000 sorted IgG+ memory B cells to amplify >>IGHV4-59 and IGLV3-21 gene families from which the PGT121–124 and PGT133–134 antibodies were derived [5]. PGT124, PGT133, and PGT134 are somatic variants of PGT121–123 monoclonal antibodies (mAbs) that were recently isolated through direct functional screening and by antigen-based cell sorting (Figure 1). These libraries were then submitted to 454 sequencing to yield a total of 376,114 heavy-chain and 530,197 light-chain reads.
To define unique clones, the V and J gene for each read was determined along with its percent mutation from the corresponding germline sequence. We then analyzed the clustering quality using multiple values for the cutoff range. The distribution of cophenetic distances in the hierarchical clustering linkage tree showed that a cutoff of 4–5 “edits” was optimal, because at that distance, the distribution had a qualitative change most likely associated with sequencing error. Given the average length of the International Immunogenetics (IMGT)-defined complementary determining region 3 (CDR3), 4–5 edits corresponds to a 90% identity using USEARCH's iddef 1 option [31]. Target PGT antibody sequences were manually selected for further analysis as follows. Each read was scored on its identity to a PGT antibody and on its mutation level from the IMGT reference germline sequence. The vast majority of reads formed several large, low-identity clusters in a divergence-mutation scatter plot; in some cases, there existed small clusters of high-identity reads separate from the large clusters (Figure 2A–B and Figure S1, S2). These small clusters were manually extracted and carried forward for phylogeny. This approach identified a total of 97 heavy-chain and 530 light-chain sequences as PGT121–134 variants. The greater number of light chain variants is likely due partly to more raw reads from the sequencing run and partly to library preparation, which has also been observed by other deep sequencing studies of HIV antibody repertoires [6], [32]. Indeed, the primers we used to amplify the heavy chains were not as specific as the light chain primers and so there may have been less enrichment for heavy chain sequences. Sequence variants were then used to model the history of the PGT121–134 antibody lineage so as to experimentally characterize early less-mutated variants. We designed a new Bayesian phylogeny algorithm, ImmuniTree, to model the SHM of this lineage (Text S1).
Existing approaches to phylogeny were suboptimal for our experiment because of two principal issues. Firstly, existing algorithms treat 454 read errors as true somatic mutations, thereby leading to excessive branching in the phylogeny and difficulties in interpretation. ImmuniTree addresses this obstacle by taking advantage of the distinctly different nucleotide transition characteristics between clonal SHM and 454 sequencing errors. Secondly, traditional phylogeny algorithms do not explicitly model whether or not to generate an internal node. Instead, these algorithms either always infer an internal unobserved intermediate (e.g. neighbor joining), or never do so (e.g. minimal spanning trees). This poor representation inaccurately models reality, where noisy yet highly clonal data are observed from only a subset of leaves and internal clones.
Though emerging methods address only one or neither of these issues [33], ImmuniTree addresses both explicitly (Text S1). For each antibody clone, the algorithm models SHM as a birth-death model, where reads are generated from clonal populations using a known sequencing error model. The algorithm jointly models sequencing error alongside somatic mutation and collapses reads that differ due to sequencing error. The approach reduces inference variance and facilitates human interpretation when choosing sequences for the subsequent evaluation of binding and neutralization.
The ImmuniTree algorithm relies on a probabilistic model that describes both the expansion process of a clone and the sequencing process. It iteratively explores this probabilistic space using the Markov chain Monte Carlo framework [34], producing a new hypothetical tree in each iteration, aimed at describing how the data were generated. In the hypothesized tree, each node is associated with one sequence, and the reads are associated with the nodes of the tree. The disagreements between the sequence of a node and its parent represent new hypermutations. The disagreements between a read and the sequence of its node represent sequencing errors. The mechanics of the algorithm guide the search to high-likelihood areas of the probabilistic space. During each iteration the tree is constantly manipulated, intermediate nodes are stochastically added and deleted, and if such nodes contribute to a tree that better explains the data, they are likely to stay. Then, the DNA sequences of all the nodes in the hypothesized tree, including intermediates, are jointly updated by an exact inference algorithm [35]. At the end of the search, the algorithm returns the tree with the highest likelihood.
Given the tree topology, and the association of reads to nodes in the tree (the “evidence”), we use belief propagation algorithm to infer the sequences of all the nodes, including intermediate nodes. The belief-propagation algorithm is a message-passing algorithms, a generalization of the backward-forward algorithm for hidden Markov models (HMMs), which computes the set of sequences that yield the highest likelihood according to the mutation model.
Each node in the tree receives “messages” from its children stating their best estimate for its sequence. Each child's estimate is based on the evidence in its subtree, taking into account the mutation model. Those messages are integrated with the evidence at the node itself into a new message that is sent to its own parent, estimating the parent's sequence. Once the root of the tree received all the messages from its children, it sends to each of its children its best estimate, now fully informed, for their sequence. This process is repeated until all the nodes in the tree are instantiated with their optimal sequences, given to them by their parent.
When applied to the PGT121–134 variants, ImmuniTree generated trees for heavy and light chain sequences with a total of 37 nodes (named 1H through 37H) and 120 nodes (named 1L through 120L), respectively (Figure 2C–D). Within these two trees, 13 internal nodes in the heavy chain tree and 28 internal nodes of the light chain tree were inferred statistically, and did not have direct sequencing data (Figure 2C–D). As expected, trees built without correcting for sequencing error resulted in a larger number of nodes and branches, which confounds lineage analysis (Figure S3). Overall, compared to the inferred internal nodes, the observed nodes in both heavy and light chain trees were relatively highly mutated with an average divergence from germline of 18% for VH and 24% for VL. The observation that only highly mutated sequences were found suggests that early precursors might be absent in the B cell memory compartment or are present at very low levels, possibly because the donor blood samples were obtained several years into the infection. Indeed, this observation is consistent with other antibody lineage studies for which inferred intermediates were calculated because less-mutated sequences were absent or were present at very low frequencies in the sequencing dataset in both late time point samples and even in early blood draws from longitudinal studies [6], [27], [32]. We were particularly interested in whether these less-mutated inferred precursors could also exhibit broad neutralization.
Neutralization activity positively correlates with degree of SHM
To see if observed and inferred sequences were capable of generating functional antibodies, we paired heavy and light chain sequences from nodes of varying mutation levels and measured neutralization activity on a cross-clade 6-virus panel to evaluate neutralization breadth and potency (Figure 3) [36]. As shown in a recent study, the PGT121 family of antibodies retained full function only when heavy and light chains within the lineage were paired and were not functional or suffered a great reduction in neutralization and binding when paired with heavy or light chains from other N332-specific antibodies [37]. Moreover, the structural similarity between somatic variants suggests that heavy and light combinations within the lineage should give rise to antibodies capable of binding and/or neutralization [38], [39]. Indeed, the variants and putative intermediates selected in this study share many of the amino acid motifs characteristic of this family of antibodies and are therefore presumed to fold into similar antibody structures. Thus, while the heavy and light chain pairs presented here may not recapitulate natural in vivo pairing, the differences in SHM levels between chimeras can still offer insight into the contribution of SHM to the antibody lineage's neutralization breadth and potency.
The mutation levels of the selected clones in comparison to known heavy and light chain pairs from this donor are listed in Figure 1. We selected the intermediates based only on mutation frequency. For example, we chose the least mutated nodes 3H for heavy chain and 3L for light chain to determine if neutralization activity was achievable at these levels of SHM. Additionally, we chose nodes that followed consecutively in SHM levels (e.g. 9H to 10H to 13H for heavy chain and 84L to 87L to 109L for light chain) to determine if the level of SHM correlates with neutralization breadth and/or potency. Surprisingly, pairing of the least mutated nodes (3H+3L) resulted in a variant that demonstrated notable neutralization activity (Figure 3). Also, antibody clones comprising heavy and/or light chain sequences that are more divergent from germline consistently demonstrated greater neutralization potency and/or wider breadth on the 6-virus panel (Figure 3 and Figure S4). Some combinations from observed sequences, however, were unable to demonstrate neutralizing activity, which could be due to either incorrect chain pairing or the accumulation of deleterious mutations during SHM that rendered them no longer functional.
To investigate further, we chose to characterize putative intermediates for the affinity matured PGT121 and PGT124 antibodies, which lie on different branches of the evolutionary tree and have slightly different neutralization profiles (Figure 2C–D and Figure S5, S6). As an inferred precursor to both antibodies, we chose the least mutated nodes 3H from the heavy chain tree and 3L from the light chain tree. To investigate intermediate levels of SHM, we chose 9H as a heavy chain precursor of PGT121–123 and 32H as a precursor of PGT124. Finally, we also tested pairing with node 87L from the light chain tree as it demonstrated very high neutralization breadth and potency when paired with various heavy chains.
With a mutation frequency of 5% in VHJH and 9% in VLJL (overall nt mutation frequency of 6%), the antibody 3H+3L nevertheless neutralized 46% of a broader panel of 74 viruses sensitive to PGT121/PGT124 with a median IC50 only 24- and 14-fold higher than PGT121 and PGT124, respectively (Figure 4A–B, Figure 5A and Figure S7, S8). With a few additional nucleotide mutations in the heavy chain (11% in VHJH, overall nt mutation frequency of 10%), the 32H+3L pair neutralized 80% of viruses that are sensitive to PGT124 with only 1.5-fold higher median IC50 than PGT124. The inferred intermediate for PGT121, 9H+3L, is 12% mutated in VHJH (also 10% nt mutation frequency overall), but demonstrated less breadth and potency neutralizing 62% of PGT121-sensitive viruses with a 12-fold higher median IC50 than PGT121. Overall, the results confirm that, while SHM does enhance neutralization breadth and potency for this family of antibodies (Figure 4A–B), we are still able to observe relatively high levels of broad neutralization activity with substantially lower levels of SHM than found for the most affinity-matured antibodies.
To better evaluate the neutralization activity of the antibodies of intermediate divergence, 3H+3L and 32H+3L were tested on a 103 cross-clade virus panel in comparison with b12 and PGT121 (Figure S9, S10). b12 was one of the first CD4bs bNAbs isolated and demonstrates moderate neutralization breadth compared to more recently isolated bNAbs [3], [5]. The results show that the least mutated pair 3H+3L is 6% less mutated than b12, but demonstrated equivalent breadth (26%) and its median IC50 was still 3-fold more potent than b12 and 15-fold less potent than PGT121. The pair 32H+3L, meanwhile, was capable of neutralizing 48% of viruses on this panel and its median IC50 is 15-fold more potent than b12 and only 3-fold less potent than PGT121. PGT121 was capable of neutralizing 58% of viruses on this panel. Thus, while SHM does enhance neutralization breadth and potency for this family of antibodies, we are still able to observe relatively high levels of neutralization activity with substantially lower levels of SHM than the fully matured clones.
Residues arising through SHM are important for neutralization at different stages of antibody maturation
In order to determine if residues in the variable region are a result of SHM or are due to polymorphisms in the donor, genomic DNA from the PGT121–123 donor was extracted and V and J genes from both heavy and the light chains were amplified and Sanger sequenced (Figure S11). Based on the results, there are no unique polymorphisms in the donor that distinguish the germline sequence from those reference sequences found in the IMGT database. Additionally, the sequence results indicate that the deletion in FR1 and the insertion in FR3 of the light chain, which are present in the PGT121–134 mAbs, were features that developed following VDJ or VJ recombination.
We next determined how individual mutations at different stages of antibody maturation enabled greater neutralization breadth or potency to gain insight into the most critical steps of SHM for this family of antibodies. For the antibody 3H+3L, single amino acids were individually reverted to the corresponding germline residue and residues comprising a framework light chain 3 (FRL3) insertion and CDR3 regions were individually substituted by alanine (Figure 6A–G). The parent 3H+3L mAb and mAb variants were then tested for neutralization on a 6-virus panel and the fold changes in IC50 are reported in Figure 6B and Figure S12. For the heavy chain of antibody 3H+3L, most of the neutralization activity for the majority of isolates is apparently mediated through the CDRH3; simultaneous reversion of all the heavy chain residues of 3H to germline, except for the CDRH3, resulted in loss of neutralization or reduced potency for only 20% of isolates (Figure S13). For the light chain of antibody 3H+3L, residues critical for neutralization activity were found in both framework and CDR regions, although these residues become less critical when paired with more mature heavy chains (Figure S14). Crystallographic studies of a putative PGT121 germline Fab, which is similar (0.4 Å core RMSD) to a previously reported crystal structure of another PGT121 putative germline antibody, suggested that the mutations from germline not only involved likely antigen contact residues, but also involved structural rearrangements in the heavy and, most notably, light chains as a result of somatic mutation in CDR and framework regions (Figure 6C–G, Figure S14, and Table S1) [39].
Having identified the residues mutated from germline that are most crucial to neutralization by antibody 3H+3L, we were interested in determining those that were most important to the increased breadth of antibodies 32H+3L and 3H+87L as compared to 3H+3L. Accordingly, the more mutated intermediate heavy chain 32H was reverted to the less mutated 3H heavy chain, paired with light chain 3L, and tested for neutralization breadth. A similar approach was taken for reversion of light chain 87L to 3L, paired with heavy chain 3H. Surprisingly, single amino acid changes did not show demonstrable effects on neutralization IC50 for the 32H reversion or for the 87L reversion (Figure S15). Strikingly, only variants for which at least two amino acids were reverted did we see a large increase in IC50 between parent and variant mAb in an isolate-specific manner. Such a pattern may present extra challenges to the guided evolution of an antibody response through vaccination.
To gain further insight into the effects of the insertion and deletions on neutralization activity, we tested neutralization breadth and potency of the PGT122–133 antibodies without the FRL3 insertion and restoring the framework light chain 1 (FRL1) deletion on a cross-clade 6 -virus panel. Upon removal of the FRL3 insertion, we observed a partial loss of neutralization in an isolate-specific manner that varied somewhat among the somatic variants of the lineage (Figure 7A–D). The isolate 92BR020, for example, became resistant to neutralization by PGT123 lacking the FRL3 insertion, but was still sensitive to the corresponding PGT122, PGT124, and PGT133 variants. Meanwhile, the isolate IAVI C22 became resistant to PGT122 lacking the FRL3 insertion, but remained sensitive to PGT123, PGT124, and PGT133 variants. Notably, the isolate 92RW020 remained sensitive to all the antibodies in the absence of the FRL3 insertion. These results suggest that the FRL3 insertion is beneficial but not absolutely crucial for neutralization breadth and that the antibody maturation process may have proceeded some way before the insertion occurred. These results also argue that, if an immunogen is capable of eliciting this lineage of antibodies without the FRL3 insertion, neutralization breadth may be achieved through the complementary neutralization profiles of somatic variants. Finally, we were unable to find large effects on neutralization breadth or potency upon restoration of the 7-amino acid N-terminus deletion in FRL1 suggesting that this feature is not crucial for bnAb activity (Figure 7E).
Intermediate antibody binds cell surface Env more effectively than monomeric gp120 and shows greater dependence on recognition of multiple glycans than mature PG121
Many research groups have generated germline derivatives or close equivalents (unmutated germline ancestors) of known bnAbs, but these constructs do not bind most recombinant gp120s or neutralize most virus strains [23], [25], [26], [40]. Indeed, the putative germline of PGT121 was unable to bind gp120 (Figure 8A–B). However, the intermediate 3H+3L antibody did bind weakly to monomeric gp120 from multiple isolates. Most notably, the 3H+3L antibody bound to monomeric gp120 with notably lower apparent affinity than to trimeric cell surface Env in contrast to mature PGT121, which bound with similar apparent affinities to monomeric gp120 and trimeric Env (Figure 9A–B and Figure S16). We confirmed by competition assays that, despite these binding differences, antibodies 3H+3L and PGT121 do share overlapping epitopes (Figure 10A). To determine if the preference of antibody 3H+3L for trimeric Env was due to avidity effects, we generated Fab fragments and tested neutralization activity and binding by flow cytometry. The results demonstrate that 3H+3L loses neutralization potency as an Fab compared to IgG, despite little difference in apparent affinity for cell surface trimer between Fab and IgG (Figure 10B–C). Conversely, PGT121 maintains neutralization potency as a Fab. These results suggest that the early intermediate antibodies may be crosslinking between Env on virions in order to achieve neutralization [7], but the lineage becomes less dependent on crosslinking following affinity maturation. Overall, these results indicate that the PGT121–134 antibody lineage may have been selected for native Env binding at some point during maturation, possibly on whole virions.
Previous work identified only the N332 glycan as critical for PGT121 neutralization and binding [5], [41]. However, because all of the other known glycan-binding antibodies require concomitant binding of adjacent glycans, we tested our putative precursors for involvement of other glycans in neutralization [5], [7], [42]–[44]. Reduced neutralization on N301A viruses and complete loss of neutralization on N332A viruses was found for 3H+3L, 32H+3L, and 9H+3L mAbs (Figure 11A–C). For PGT121, we observed no effects for N301A viruses, a substantial loss of neutralization for N332A viruses, and an even greater loss of neutralization for N332A+N301A mutant viruses (Figure 11D–F). Overall, the results suggest that early antibodies of the PGT121–123 lineage are dependent on both N301 and N332 glycans and that the dependency on the N301 glycan is reduced upon maturation.
Discussion
The conclusions described here can help guide immunogen design to elicit PGT121-like antibodies. Specifically, the presentation of native Env instead of monomeric gp120 in germinal centers appears to be important for the maturation of this family of antibodies. This preference for native Env over monomeric gp120 may be due to avidity effects or to the presentation of epitope residues on native Env that are not fully recapitulated on monomeric gp120. Additionally, we observed an evolution in the dependency of glycan binding between putative intermediates and fully mature antibody. As conventional protein-glycan interactions are usually in the micromolar range, the avidity offered by binding to multiple glycans may be an important factor in triggering glycan-dependent bnAb lineages. By comparing the differences in glycan contact residues between putative precursor and affinity-matured antibody, it may be possible to rationally target residues for maturation in order to achieve greater neutralization breadth.
Compared to less-mutated bnAbs that target the CD4 binding site [27] the less-mutated intermediates presented here are notably broader and more potent suggesting potentially important differences between the CD4 binding site and the N332 region as vaccine targets that may give some advantage to the latter region. One possible explanation is that antibodies targeting the CD4bs may require more SHM in order to fold into specific conformations that enable binding into the CD4bs pocket. The epitope that PGT121 binds to, on the other hand, may be more exposed and may accommodate different antibody binding angles [7], [11], [37], [38]. Thus, the PGT121 lineage may not necessarily require extensive levels of SHM in order to bind its epitope. It would likely be informative to perform similar analyses for other known bnAbs in order to better define the minimal amount of mutations necessary to achieve neutralization breadth and potency for different bnAbs.
Despite the lower levels of SHM associated with PGT121-like, more VRC01-like antibodies than PGT121-like bnAbs have been isolated from various donors. This apparent discrepancy may be due to the fact that many B cell selection studies have used a bait (RSC3) engineered for isolating VRC01-like antibodies . Additionally, there are many donors in various cohorts who show N332 serum reactivity whose antibodies have not yet been isolated and thus new PGT121-like antibodies may be forthcoming. Finally, antibodies targeting the N332 epitope appear to derive from several VHDHJH and VLJL combinations, while VRC01-class antibodies utilize only a single VH germline gene, the VH1-2 gene. Thus, given the greater diversity of antibody responses targeting the N332 epitope, the probability of identifying an exact or highly similar PGT121 response from other infected donors may be low.
Importantly, as shown here, deep sequencing and phylogeny with ImmuniTree has the potential to identify intermediate antibodies that may not be as broadly neutralizing as some individual affinity-matured Abs, but have considerably lower levels of SHM. In this regard, considerable neutralization breadth may be achieved by eliciting less-mutated antibodies with complementary neutralization profiles, probably targeting a range of epitopes. Such an approach may circumvent the need for long-term prime/boost strategies, which are likely required to elicit high levels of SHM and suggests that, given the right immunogens, more conventional vaccination strategies than have been contemplated may be sufficient for an HIV vaccine.
Strikingly, it appears that higher levels of SHM concurrently enhance neutralization breadth and increase potency against the majority of isolates recognized by putative precursors (Figure 4A–B and Figure S7, S8). These observations are consistent with the hypothesis that SHM results in some antibodies becoming both very broad and potent by “homing in” on a conserved site of vulnerability (“sweet spot”) from a response that initially recognizes a somewhat different footprint on the virus Env. One hypothesis suggests that Env escape variants drive maturation to avoid variable residues and/or glycans adjacent to the most conserved residues by directing contacts to converge on the smallest and most conserved binding site on Env (compare ref. [45]). An alternative hypothesis suggests that the antibody matures to avoid variable residues and/or glycans without substantially changing the size of its footprint but by refining its angle of approach (compare ref. [46]). By either of these mechanisms, antibody selection can occur on the basis of affinity for diverse isolates with a concomitant increase in neutralization breadth and potency (Figure 4A–B and Figure S17). Indeed, calculations of an antigen-antibody interaction model are consistent with the idea that bnAbs are characterized as antibodies that form a small number of stronger interactions with conserved residues compared to antibodies that are able to make many contacts with many variable residues (Figure S18). Further dynamic modeling of antibody SHM in response to Env escape in concert with targeted immunization studies will help to design vaccines and vaccine strategies that hone antibody responses onto the the PGT121 epitope.
Materials and Methods
Ethics statement
Peripheral blood mononuclear cells (PBMCs) were obtained from donor 17, an HIV-1 infected donor from the IAVI Protocol G cohort [36]. All human samples were collected with written informed consent under clinical protocols approved by the Republic of Rwanda National Ethics Committee, the Emory University Institutional Review Board, the University of Zambia Research Ethics Committee, the Charing Cross Research Ethics Committee, the UVRI Science and Ethics Committee, the University of New South Wales Research Ethics Committee. St. Vincent's Hospital and Eastern Sydney Area Health Service, Kenyatta National Hospital Ethics and Research Committee, University of Cape Town Research Ethics Committee, the International Institutional Review Board, the Mahidol University Ethics Committee, the Walter Reed Army Institute of Research (WRAIR) Institutional Review Board, and the Ivory Coast Comité National d'Ethique des Sciences de la Vie et de la Santé (CNESVS).
454 sequencing library preparation
Reverse transcription was performed with 10 µL total RNA and 2 µL RT primer mix (50 µM oligo-dT and 25 µM random hexamer). The mixture was heated at 95°C for 1 min, 65°C for 5 min, then cooled on ice for 1 min. For each reaction, a mix was prepared with 4 µL 5× FS buffer, 1 µL 10 mM dNTP mix, 1 µL 0.1 M DTT, 1 µL RNase inhibitor (Enzymatics), and 1 µL SuperScript III RT (Invitrogen). This mix was added to the reaction and incubated at 25°C for 10 min, 35°C for 5 min, 55°C for 45 min, and 85°C for 5 min. RNA/DNA hybrid was removed by adding 4 µL E. coli RNase H (Enzymatics). PCR reactions were assembled using 13.75 µL water, 5 µL cDNA, 5 µL 5× HF buffer, 0.5 µL 10 mM dNTP, 0.25 µL of each 100 µM primer stock, and 0.25 µL Phusion Hot Start. The reaction was cycled at 98°C (60 s), 24 cycles of 98°C (10 s), 62°C (20 s), and 72°C (20 s), with a final extension at 72°C (5 min). Samples were purified on a QIAquick column and run on a 2% agarose E-gel. The desired bands were purified using the Qiagen MinElute gel extraction kit, eluted twice with 10 µL EB buffer, and quantitated on a 2100 Bioanalyzer. Samples were sent to SeqWright for 454 sequencing, which was performed per manufacturer's instructions.
Pseudovirus production and neutralization assays
To produce pseudoviruses, plasmids encoding Env were co-transfected with an Env-deficient genomic backbone plasmid (pSG3ΔEnv) in a 1∶2 ratio with the transfection reagent Fugene 6 (Promega). Pseudoviruses were harvested 72 hours post transfection for use in neutralization assays. Neutralizing activity was assessed using a single round of replication pseudovirus assay and TZM-bl target cells, as described previously [5].
Cell sorting and RNA extraction
Frozen vials of 10×106 PBMCs were thawed and washed before staining with Pacific Blue labeled anti-CD3 (UCHT1), Pacific Blue labeled anti-CD14 (M5E2), FITC labeled anti-CD19 (HIB19), PE-Cy5 labeled anti-CD10 (HI10a), PE labeled anti-CD27 (M-T271), and APC labeled anti-CD21 (B-ly4), all from BD Biosciences. Sorts were performed on a high speed BD FACSAria into miRVana lysis buffer (Ambion). Immature B cells, exhausted tissue-like memory, activated mature B cells, resting memory B cells, and short-lived peripheral plasmablasts were stained using previously described markers [47]. Total RNA from the cells was then extracted using the mirVana RNA extraction kit (Ambion) according to manufacturer's instructions and quantitated on a 2100 Bioanalyzer (Agilent).
Antibody and protein expression and purification
Antibody sequences were synthesized and cloned into previously described heavy and light chain vectors [3], [48]. The plasmids were co-transfected (1∶1 ratio) in either HEK 293T or 293 FreeStyle cells using Fugene 6 (Promega) or 293fectin (Invitrogen), respectively. Transfections were performed according to the manufacturer's protocol and antibody supernatants were harvested four days following transfection. Antibodies produced in 293T cells were quantified by ELISA and used directly in neutralization assays. Antibodies produced in 293 freestyle cells were further purified over a protein A column. Mutations were introduced by site-directed mutagenesis using the QuikChange site-directed mutagenesis kit (Stratagene). Recombinant gp120 proteins were transfected in 293 FreeStyle cells using 293fection (Invitrogen) and purified with Galanthus nivalis lectin column followed by size exclusion using Superdex 300 26/60 (GE Healthcare).
Cell surface binding assays
Titrating amounts of mAbs were added to HIV-1 Env-transfected 293T cells and incubated for 1 h at 4°C in 1× PBS. Following washing, cells were fixed with 2% PFA (PolySciences) for 20 min at RT. The cells were then washed and stained with a 1∶200 dilution of phycoerythrin-conjugated goat anti-human IgG F(ab′)2 (Jackson) for 1 h at RT. Binding was analyzed using flow cytometry. Binding competitions were performed by titrating amounts of competitor monoclonal antibodies before adding biotinylated antibody at a concentration required to give IC70 and then measuring binding with phycoerythrin-labeled streptavidin (Invitrogen). FlowJo software was used for data interpretation.
ELISA assays
Binding by ELISA was performed as described previously [5]. Briefly, plates were coated with goat anti-human IgG Fc (Pierce) or with gp120 and binding was detected using goat anti-human IgG F(ab′)2 conjugated to alkaline phosphatase (Pierce). For binding to gp120 extracted from lysed virions, plates were coated with 5 ng/uL of sheep D7324 anti-gp120 antibody (Aalto Bio reagents). Virus supernatents were lysed using a final concentration of 1% NP-40 and incubated on coated plates for 2 h at 37°C. Detection was measured using goat anti-human IgG F(ab′)2 conjugated to alkaline phosphatase (Pierce). Antibody concentration was calculated by linear regression using a standard concentration curve of purified IgG protein.
PGT121 germline Fab expression, purification, crystallization and X-ray diffraction analysis
PGT121germline Fab was produced in HEK 293T cells and purified as previously described [7]. Briefly, three days after transfection with the heavy and light chain genes, the expression media was harvested and the secreted Fab was purified via an anti-human λ light chain affinity matrix (CaptureSelect Fab λ; BAC), followed by cation exchange chromatography and size-exclusion chromatography. X-ray diffraction quality crystals were obtained in a condition containing 0.2 M magnesium acetate, 20% w/v PEG 8000, 0.1 M sodium cacodylate, pH 6.5.Before mounting and flash freezing the crystals in liquid nitrogen, the mother liquor was supplemented with 20% glycerol for cryo-protection. A complete datasetwas collected at the APS 23-ID beamline. Data processing was performed using XDS [49]. The PGT121germline structure was solved using PHASER in space groups P212121 with the PGT123 Fab structure as a search model [50]. Refinement was performed using a combination of PHENIX and COOT [51], [52]. Refinement statistics for the final models are reported in Table S1.
Raw data processing: VDJ alignment and clone definition
Raw sequencing data were processed using in-house tools written in python. Reads were split into barcodes, size-filtered, and aligned to IMGT's germline VDJ reference database. The scores were kept low, as we were interested in sequences that were very highly mutated. The V region is aligned first, then removed, followed by J, then removed, followed by D. The IMGT-defined CDR3 sequence of each read was then extracted. The CDR3 sequences were sorted by abundance and clustered with USEARCH5.1 with the options “–minlen 0 –global –usersort –iddef 1 –id 0.9”. Finally, each CDR3 sequence was aligned to the “target” antibody sequences of PGT121–123 to determine a “divergence” value from these antibodies.
Antibody variant identification and analysis
The divergence-mutation plots are used as a tool to identify reads that are similar to the known PGT121–123 antibodies. High-identity clusters of sequences and clusters that are above background are manually identified and used as input for a phylogeny inference with Immunitree. Immunitree implements a Bayesian model of somatic hypermutation of clones, including probabilistic models of SHM and sequencing error and performs Markov chain Monte Carlo over the tree structure, birth/death times of the subclones, birth/death, mutation, and sequencing error rates, subclone consensus sequences, and assignment of reads to nodes. The tree structure is also used for multiple computations and to overlay different information. We estimate the selection pressure that a given node has experienced using the BASELINe algorithm. It performs a Bayesian estimation of selection pressure by comparing the observed number of replacement/silent mutations in the CDRs/FWRs of the node consensus sequence.
Supporting Information
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Štítky
Hygiena a epidemiologie Infekční lékařství LaboratořČlánek vyšel v časopise
PLOS Pathogens
2013 Číslo 11
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