Analysis of cell-type-specific chromatin modifications and gene expression in Drosophila neurons that direct reproductive behavior

English version

Autoři: Colleen M. Palmateer ^aff001; Shawn C. Moseley ^aff001; Surjyendu Ray ^aff001; Savannah G. Brovero ^aff001; Michelle N. Arbeitman ^aff001
Působiště autorů: Department of Biomedical Sciences, Florida State University, College of Medicine, Tallahassee, Florida, United States of America ^aff001; Program of Neuroscience, Florida State University, Tallahassee, Florida, United States of America ^aff002
Vyšlo v časopise: Analysis of cell-type-specific chromatin modifications and gene expression in Drosophila neurons that direct reproductive behavior. PLoS Genet 17(4): e1009240. doi:10.1371/journal.pgen.1009240
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1009240

Souhrn

Examining the role of chromatin modifications and gene expression in neurons is critical for understanding how the potential for behaviors are established and maintained. We investigate this question by examining Drosophila melanogaster fru P1 neurons that underlie reproductive behaviors in both sexes. We developed a method to purify cell-type-specific chromatin (Chromatag), using a tagged histone H2B variant that is expressed using the versatile Gal4/UAS gene expression system. Here, we use Chromatag to evaluate five chromatin modifications, at three life stages in both sexes. We find substantial changes in chromatin modification profiles across development and fewer differences between males and females. Additionally, we find chromatin modifications that persist in different sets of genes from pupal to adult stages, which may point to genes important for cell fate determination in fru P1 neurons. We generated cell-type-specific RNA-seq data sets, using translating ribosome affinity purification (TRAP). We identify actively translated genes in fru P1 neurons, revealing novel stage- and sex-differences in gene expression. We also find chromatin modification enrichment patterns that are associated with gene expression. Next, we use the chromatin modification data to identify cell-type-specific super-enhancer-containing genes. We show that genes with super-enhancers in fru P1 neurons differ across development and between the sexes. We validated that a set of genes are expressed in fru P1 neurons, which were chosen based on having a super-enhancer and TRAP-enriched expression in fru P1 neurons.

Klíčová slova:

Behavior – Gene expression – Gene ontologies – Histone modification – Chromatin – Chromatin modification – Neurons – Exon trapping

Zdroje

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