Vliv zinečnatých a kademnatých iontů na růst a produkci kumarinů v suspenzní kultuře Angelica archangelica L.

English version

Autoři: Tomáš Siatka; Marie Kašparová; Jiřina Spilková
Působiště autorů: Charles University in Prague, Faculty of Pharmacy in Hradec Králové, Department of Pharmacognosy
Vyšlo v časopise: Čes. slov. Farm., 2012; 61, 261-266
Kategorie: Původní práce

Souhrn

Rostlinná buňka může reagovat na nadbytek těžkých kovů ve svém prostředí různými mechanismy, včetně zvýšené biosyntézy sekundárních metabolitů. V této práci byly testovány zinečnaté (0 až 1500 μM) a kademnaté (0 až 100 μM) ionty jako potenciální elicitory produkce kumarinů v suspenzních kulturách anděliky lékařské. Navíc byla posuzována toxicita obou kovů hodnocením jejich účinku na buněčný růst (charakterizován čerstvou a suchou hmotností biomasy na konci čtrnáctidenní kultivace). Bylo zjištěno, že čerstvá hmotnost nebyla ovlivněna zinkem do koncentrace 150 μM u kultur ve tmě a 300 μM na světle. Potom klesala s rostoucí hladinou zinku. Zinek v koncentraci 1500 μM ji snížil v kulturách rostoucích ve tmě o 54 %, za světla o 24 %. Suchá hmota byla ovlivněna podobným způsobem. Zinek v koncentraci 1500 μM redukoval suchou hmotnost při kultivaci ve tmě o 30 %, na světle o 20 %. Kademnaté ionty neovlivnily čerstvou a suchou hmotnost buněk u kultur ve tmě do koncentrace 10 μM, na světle do 50 μM. Toxické koncentrace kadmia jsou o řád nižší než u zinku. Kadmium v koncentraci 50 μM snížilo čerstvou hmotnost buněk o 66 %, suchou o 59 % v kulturách ve tmě. Kadmium v koncentraci 100 μM redukovalo čerstvou hmotnost buněk o 40 %, suchou o 44 % v kulturách na světle. Zinečnaté ani kademnaté ionty nezvýšily produkci kumarinů.

Klíčová slova:
Angelica archangelica L. • suspenzní kultura • růst • kumariny • zinek • kadmium • elicitace • světelné podmínky • sekvenční injekční analýza

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