Ifitm3 Limits the Severity of Acute Influenza in Mice

Summary

Interferon-induced transmembrane (IFITM) proteins are a family of viral restriction factors that inhibit the entry processes of several pathogenic viruses, including influenza A virus (IAV), in vitro. Here we report that IAV-infected knockout mice lacking the Ifitm locus on chromosome 7 exhibited accelerated disease progression, greater mortality, and higher pulmonary and systemic viral burdens as compared to wild type controls. We further observed that the phenotype of Ifitm3-specific knockout mice was indistinguishable from that of mice lacking the entire Ifitm locus. Ifitm3 was expressed by IAV target cells including alveolar type II pneumocytes and tracheal/bronchial respiratory epithelial cells. Robust Ifitm3 expression was also observed in several tissues in the absence of infection. Among murine Ifitm promoters, only that of Ifitm3 could be induced by type I and II interferons. Ifitm3 could also be upregulated by the gp130 cytokines IL-6 and oncostatin M on cells expressing appropriate receptors, suggesting that multiple cytokine signals could contribute to Ifitm3 expression in a cell or tissue-specific manner. Collectively, these findings establish a central role for Ifitm3 in limiting acute influenza in vivo, and provide further insight into Ifitm3 expression and regulation.

Introduction

The interferon-induced transmembrane (IFITM) proteins are a family of small transmembrane proteins that mediate some of the antiviral activities of type I and II interferons [1], [2], [3]. IFITM-mediated restriction is specific to particular virus families. Influenza A viruses (IAV), Ebola virus (EBOV), Marburg virus (MARV), SARS coronavirus (SARS-CoV), dengue virus, and West Nile virus are all efficiently restricted by one or more IFITM family proteins. Conversely, IFITM proteins do not restrict murine leukemia virus (MLV), a range of alphaviruses, and arenaviruses including Machupo virus (MACV), Lassa virus (LASV), and lymphocytic choriomeningitis virus (LCMV) [2], [4], [5]. Vesicular stomatitis virus is inefficiently restricted [6], [7]. Two groups have reported cell line-specific restriction of HIV-1 [1], [8], but these reports differ as to which cell lines show efficient restriction. Restriction activity against non-enveloped viruses has not been reported to date.

The mechanism of action of IFITM-mediated restriction has not yet been determined. Multiple lines of evidence indicate that restriction occurs after virion endocytosis but during or prior to membrane fusion. IFITM proteins prevent infection by retroviruses pseudotyped with entry proteins of IFITM-restricted viruses, but not those pseudotyped with entry proteins of unrestricted viruses [4]. Entry assays using virions containing β-lactamase-Vpr (BlaM-Vpr) fusion proteins likewise reveal that IFITM proteins prevent transfer of BlaM-Vpr to the cytosol of target cells [8]. Previously, we showed by fluorescent microscopy that labeled IAV virions are internalized normally and traffic to acidified endocytic compartments despite IFITM expression [4], a conclusion confirmed and extended by Feely et al. using a rigorous set of imaging studies. They further demonstrated by fluorescent in situ hybridization that IFITM proteins cause sequestration and accumulation of IAV genetic material in late endocytic/lysosomal compartments [9].

IFITM proteins also localize to late endosomes/lysosomes as demonstrated by co-localization with LAMP1, LAMP2, and CD63 [4]. Moreover, IFITM protein over-expression – and in some cell lines, interferon treatment – results in enlargement of these organelles [9]. Finally, a recent report showed that endosomes in interferon-treated Ifitm knockout cells are inefficiently acidified and suggested that a physical association between Ifitm3 and a subunit of the vacuolar ATPase complex mediates this effect [10]. It appears, therefore, that IFITM proteins alter the properties of late endosomes and lysosomes, and render these organelles inhospitable to viral fusion. Such a model is supported by the pattern of IFITM-mediated restriction; excepting HIV-1, restricted viruses generally fuse late in the endocytic pathway whereas unrestricted viruses fuse at the plasma membrane or in early endocytic compartments. Further evidence that restriction depends on the site of viral fusion comes from investigation of SARS-CoV. SARS-CoV requires lysosomal cathepsins to activate its entry protein and fuse with the lysosomal membrane [11]. Treating receptor-bound virions with trypsin, however, removes this cathepsin dependency, induces fusion at the plasma membrane, and bypasses IFITM-mediated entry restriction [4].

Most vertebrates have two or more IFITM genes [12]. The human IFITM family is composed of four functional genes, IFITM1, 2, 3, and 5. Murine Ifitm1, 2, 3, and 5, located on chromosome 7, are clear orthologs of their human counterparts but mice possess two additional loci: Ifitm6, also located on chromosome 7, and Ifitm7, a retrogene on chromosome 16 [13]. The proteins expressed by these genes restrict influenza A virus (IAV), filovirus, flavivirus, and SARS coronavirus entry in vitro with varying efficiencies [4], [6]. Ifitm5 expression is limited to bone [14], [15], and the roles of Ifitm6 and 7 are not yet clear. Two strains of knockout mice were used in the following experiments. IfitmDel mice lack Ifitm1, 2, 3, 5, and 6 and Ifitm3egfp mice lack Ifitm3 alone [13].

The aim of the present study was to determine the in vivo contribution of the Ifitm proteins to the innate immune control of IAV. We show that Ifitm3 alone makes a significant contribution to the control of influenza in mice and provide further insight into its expression and regulation.

Results/Discussion

Murine Ifitm proteins are protective against influenza in vivo

To determine the in vivo relevance of Ifitm proteins, we challenged cohorts of IfitmDel, wild type, and heterozygous male mice with intranasal doses of either 500 or 1000 PFU of influenza A/PR/8/34 (H1N1) (PR8). In accordance with institutional policies, mice were considered moribund and euthanized upon loss of 20% of initial body weight, defined as the 2-day average of their pre-inoculation weights. At both challenge doses, knockout mice exhibited significantly accelerated disease progression and mortality compared to wild type controls (Figure 1A). Heterozygotes had an intermediate phenotype. A modest but insignificant difference was observed in the mean survival time of knockout mice receiving each challenge dose, with mice receiving 1000 PFU surviving 0.7 days longer. With the 500 PFU challenge dose, two of five wild type animals survived acute infection but disease remained uniformly lethal in knockout mice. Weight loss at days 3 and 5 was significantly increased in IfitmDel and heterozygous animals as compared to wild type controls (Figure 1B; plots of body weight in Figure S1A).

Weight loss in wild type animals receiving a 500 PFU challenge followed trends reported by other investigators [16]. Price et al. found that mice generally maintained body weight until day 4–5 post-infection, after which they underwent a rapid period of weight loss that continued until day 8–9. The rapid clinical progression beginning on day 4–5 coincided with the onset of cellular and humoral immunity while recovery on day 8–9 corresponded to clearance of the virus. In contrast to wild type animals, IfitmDel mice began a steep decline in weight beginning on day 2 consistent with a failure of innate control of early infection. The variability in the rate of disease progression of knockout mice was also far lower than that of both wild type and heterozygous animals (Figure S1A). These results demonstrate that Ifitm proteins mediate innate immunity to influenza A virus during acute infection and that the effect of their deletion is powerful enough to overwhelm other sources of experimental variation. The intermediate phenotype of heterozygous animals shows that gene dosage is important for Ifitm protein-mediated viral restriction. The requirement for high gene dosage may also explain the duplication of the Ifitm alleles observed in multiple species.

Viral loads are higher in infected IfitmDel knockout mice

Viral loads were measured in a separate cohort of mice euthanized three days after infection, the period during which body weights of wild type and IfitmDel animals diverge (Figure S1A). Weight loss trends in this cohort of animals were similar to those of previous cohorts (Figure S1B). Viral loads, as assessed by real-time RT-PCR of lung RNA, were 2.7-fold higher on average (p = 0.0159) in IfitmDel mice as compared to wild type controls (Figure 1C). Differences in splenic viral loads (Figure 1C) were similarly increased (15.9-fold, p = 0.0079).

Although the relative difference in splenic viral loads was high, IAV genome copy numbers in the spleen were on the order of 10⁴ to 10⁵ times lower than those in the lung (Figure S1C) and genome expression in the spleens of wild type animals was barely above the detection threshold of our assay. Similarly, we did not identify splenic lesions or any other lesions beyond those in the lungs, trachea, and nasal passages, consistent with the dependence of PR8 hemagglutinin on respiratory proteases for cleavage and activation [17], [18], [19], [20]. We therefore speculate that increased splenic viral loads in knockout mice are a result of more robust infection in the lung and subsequent leakage of virus or viral debris into the periphery.

Ifitm3-specific and IfitmDel knockout mice are equally susceptible to IAV infection

Previously, we reported that murine embryonic fibroblasts (MEFs) derived from IfitmDel knockout mice are highly susceptible to IAV and that both baseline and interferon-induced Ifitm protein expression contribute to the IAV resistance in wild type MEFs [2]. Ifitm3 expression alone was sufficient to restore IAV entry restriction to knockout fibroblasts [9]. We have shown that over-expression of most murine Ifitm proteins in a human cell line confers some level of IAV restriction but that Ifitm3 is most effective while Ifitm5, 6, and 7 posses comparatively little activity [4]. We confirmed this observation in the context of IfitmDel MEFs (Figure S2A); again murine Ifitm3 demonstrated the most potent IAV restriction activity regardless of HA type, likely in part due to its higher steady-state expression level (Figures S2B and S2C).

Although Ifitm3 most effectively restricts IAV in vitro, most human and murine Ifitm proteins restrict IAV to some extent when over-expressed [4]. To determine the specific in vivo contribution of Ifitm3, we performed an additional intranasal challenge of wild type, IfitmDel, and Ifitm3-specific (Ifitm3egfp) knockout mice with 500 PFU of PR8. Mortality and weight loss trends of IfitmDel and wild type animals were similar to those of Figure 1 above. Survival times of Ifitm3-specific knockout mice were not significantly different from those of IfitmDel knockout mice (p = 0.5380) and the weight loss curves for these two genotypes were superimposed (Figures 2A and B). As with the IfitmDel mice, heterozygotes of the Ifitm3-specific knockout mice exhibited an intermediate phenotype. We therefore conclude that, among Ifitm proteins, only Ifitm3 makes a substantial contribution to PR8 resistance in vivo.

Ifitm3 expression is both cell-type and cytokine dependent

Because the Ifitm3 promoter is sensitive to interferon signaling we reasoned that other Jak/STAT-mediated cytokines might likewise induce Ifitm3 expression. We tested the ability of a type III interferon (IFNλ2), the acute phase cytokine IL-6, and oncostatin M (OSM), a cytokine released from activated dendritic cells that synergizes with type I interferon [21], to upregulate Ifitm3 expression. The latter two molecules belong to a diverse family of cytokines that signal through hetero- or multimeric receptors that activate the Jak/STAT pathway via a common gp130 subunit [22]. Consistent with previous studies, type I and type II interferons induced Ifitm3 expression in both NIH 3T3 murine fibroblasts, and the murine macrophage cell line, RAW264.7. IFNλ2 was ineffective, likely due to lack of the appropriate receptor. In 3T3 cells, however, oncostatin M (OSM) induced strong Ifitm3 expression and IL-6 was a potent inducer of Ifitm3 expression and in RAW264.7 cells (Figure 3A). The ability of these two cytokines to modulate Ifitm3 expression correlated with the surface expression of their cognate receptors (Figure 3B), and was not due to increased expression of type I or II interferons (Figure S3). These data raise the possibility that other gp130-mediated cytokines such as IL-11, IL-27, and LIF could modulate basal and inducible Ifitm3 expression in cells bearing appropriate receptors. Furthermore, they indicate greater specificity and complexity in the regulation of Ifitm3 expression, and possibly other interferon-stimulated genes, than previous studies have implied.

Human IFITM1, 2, and 3 are interferon-stimulated genes [23]. Murine Ifitm3 is shown to be interferon-inducible but the regulation of the other murine Ifitms, particularly the mouse-specific family members Ifitm6 and 7, has not been studied. Therefore, we assayed the responsiveness of the murine Ifitm promoters to both type I and type II interferons (Figure 3C). We observed that, of the murine Ifitm genes, Ifitm3 alone has an interferon-inducible promoter. Figure 3C also suggests that – at least under these tissue culture conditions – Ifitm promoters may be constitutively active, perhaps contributing to an intrinsic antiviral state. We validated the results of this promoter assay by analyzing cytokine-mediated upregulation of murine Ifitm1, 2, and 3 transcripts by qRT-PCR. Consistent with the promoter studies, and in contrast to the behavior of their human orthologs, murine Ifitm1 and 2 were unresponsive to both type I and type II interferons. Ifitm3 transcript levels were significantly upregulated by both IFNα2 and IFNγ in both NIH 3T3 and RAW264.7 cells. Ifitm3 was similarly induced by OSM in 3T3 cells and IL-6 in RAW cells consistent with both western blot and promoter data (Figure 3D). Ifitm1 also appeared to respond to OSM stimulation although this increase was not statistically significant.

Ifitm3 localization is consistent with its role as an IAV restriction factor

The data in Figures 2 and 3 show that Ifitm3 is the most potent IAV restriction factor of the murine Ifitm proteins and that Ifitm3 expression alone is efficiently induced interferons. To determine where and under what conditions Ifitm3 is expressed in vivo, we performed comprehensive immunohistochemical staining of the lungs of infected and uninfected wild type mice to determine baseline and influenza-induced patterns of Ifitm3 expression. Contrary to expectations, constitutive expression of Ifitm3 was seen in many lung tissues; marked induction was seen only in the respiratory epithelium of lower airways (Figure 4A, B, C). The pattern of distribution was similar in the remaining lung tissue in IAV-infected and uninfected mice. Constitutive expression was observed in respiratory epithelial cells of the upper airways (Figure 4D, E, F), the visceral pleura (Figure 4G, H, I), and in leukocytes (Figure 4J, K, L), suggesting that Ifitm3 may provide protection against influenza prior to the interferon and acute-phase responses. No immunostaining of Ifitm3-specific knockout mice was observed, confirming the specificity of the antibody for Ifitm3 (Figure S4).

We also localized Ifitm3 expression specifically to influenza A virus target cells – alveolar type II pneumocytes and ciliated respiratory epithelial cells [24] – by immunofluorescence and confocal microscopy. Type II pneumocytes were identified by DC-LAMP expression, a marker of type II pneumocyte-specific lysosome-related organelles [25]. Ifitm3 is not only expressed by DC-LAMP positive cells but also colocalizes with DC-LAMP itself, consistent with previous in vitro reports of its localization to endosomal/lysosomal membranes (Figure 5A). In contrast, ciliated respiratory epithelium shows intense labeling of the apical cytoplasm or plasma membrane rather than the punctate intracytoplasmic distribution typical of lysosomes (demonstrated by lysosomal marker MAC-3; Figure 5B). Whether Ifitm3 functions differently in the context of these cells or its apical localization is the end result of endo/lysosomal exocytosis is unknown.

While this manuscript was under review, Everitt et al. published data showing that Ifitm3-specific knockout mice are more susceptible to infection with influenza A virus X31 (H3N2) than wild type controls [26]. They also identified and characterized a polymorphism in human IFITM3 associated with reduced restriction activity and found an increased prevalence of this mutant allele in patients hospitalized for influenza. Our data support and extend the core conclusions of their mouse studies. We further show that mice lacking entire Ifitm locus exhibit a nearly identical disease course to those lacking Ifitm3 alone, and that their respective heterozygote littermates exhibit a phenotype intermediate between knockout and wild type mice. Morever, Ifitm3 is expressed on key IAV target cells in the lungs, and among murine Ifitms, is uniquely induced by type I and II interferons. Collectively these data make clear a primary role for Ifitm3 in the control of influenza A virus in mice.

We also show that many tissues express basal levels of Ifitm3 proteins, and that at least two gp130-family cytokines – OSM and IL-6 – can induce its expression, even in the absence of an interferon response. These results suggest greater complexity in the in vivo regulation of other well-characterized interferon-stimulated genes as well. We further noted Ifitm3 expression in cell populations that are not major IAV targets such as macrophages and endothelial cells. This implies that Ifitm3 deletion will likely enhance susceptibility to infections by monocyte-tropic flaviviruses and macrophage- and endotheliotropic filoviruses.

Our results may also have implications for treatment of viral infections. The fact that Ifitm3 expression can be induced by gp130-mediated cytokines may allow for modulation of its expression in a more targeted, less pleiotropic way by agonizing or antagonizing cell type-specific gp130 heterodimeric receptors. More generally, our findings suggest that pharmacologic induction of human IFITM3 expression or emulation of IFITM3 activity could provide a broad-spectrum therapeutic approach to the treatment of a range of human pathogenic viruses.

Materials and Methods

Ethics statement

This study was carried out in strict accordance with the recommendations in the Guide for the Care and Use of Laboratory Animals of the National Institutes of Health. These studies were approved by the Institutional Animal Care and Use Committee of Harvard Medical School (Protocol Number 04743).

Influenza A virus

Human influenza virus A/PR/8 (H1N1) propagated in chicken eggs and sucrose gradient purified from allantoic fluid was obtained from Charles River Laboratories, aliquoted, and frozen. Titer was determined by standard plaque assay on Madin-Darby canine kidney cells.

Mice and inoculations

Heterozygous IfitmDel and Ifitm3egfp mice (background strain C57BL/6J-Tyrc-2J/J) were generously provided by Dr. David Adams (The Wellcome Trust Sanger Institute, Cambridge, United Kingdom). These animals were housed under specific pathogen-free conditions prior to infection. All mice were between 8 and 10 weeks old with the exception of three 12-week-old littermates (one of each genotype) used in Figure 1A. Mice were anesthetized by intraperitoneal injection of a cocktail of ketamine (2 mg per 25 g body weight) and xylazine (0.2 mg per 25 g body weight) in 200 µl of normal saline. Anesthetized mice were inoculated intranasally with influenza A virus diluted in 50 µl of phosphate-buffered saline divided evenly between both nostrils and administered over the course of one to two minutes. Animals were weighed once daily and, per institutional requirements, euthanized upon loss of 20% of initial body weight or when deemed moribund based on clinical signs. All procedures were performed with the approval of Harvard University's Institutional Animal Care and Use Committee.

Viral load measurements

Lungs and spleens were harvested, rapidly frozen, and stored at −80°C prior to RNA isolation. Tissue was thawed in RNALater, transferred to 1 ml (lungs) or 0.5 ml (spleens) of RNAase-free water and mechanically homogenized with a QIAGEN TissueRuptor. RNA extraction was performed by means of the QIAGEN RNeasy Tissue Kit and QIAshredder columns (QIAGEN). Viral loads were determined by real-time TaqMan RT-PCR. Influenza A viral M2-specific primer and probe sequences were adapted for use with IAV PR/8/34 from protocols developed by the Centers for Disease Control [27]. Sequences were: 5′-GAC CAA TCC TGT CAC CTC TGA C-3′ (forward primer), 5′-AGG GCA TTT TGG ACA AAG CGT CTA-3′ (reverse primer), and 5′-TGC AGT CCT CGC TCA CTG GGC ACG-3′ (probe). Reporter and quencher dyes were FAM and IBFQ respectively with an additional internal Zen Quencher (Integrated DNA Technologies). Similarly labeled Gapdh primer and probe sequences were: 5′-GTG GAG TCA TAC TGG AAC ATG TAG-3′ (forward primer), 5′-AAT GGT GAA GGT CGG TGT G-3′ (reverse primer), and 5′-TGC AAA TGG CAG CCC TGG TG-3′ (probe) (Integrated DNA Technologies). 25 µl reactions containing 25 ng RNA, 50 pmol each primer, and 25 pmol probe were prepared using the Superscript III One-Step RT-PCR System with Platinum Taq DNA Polymerase and ROX (Invitrogen). Cycling conditions were: 50°C for 30 min (reverse transcription), 95°C for 2 min (denaturation), and 45 cycles of 95°C for 15 seconds (dentaturation) and 55°C for 45 sec (annealing, extension, and fluorescence acquisition). Cycling and fluorescence detection were performed in an ABI Prism 7500 thermal cycler (Applied Biosystems).

Pseudovirus entry assays

Entry assays were performed on murine embryonic fibroblasts (MEFs) derived from IfitmDel knockout mice. Cells were transduced with retroviral puromycin-selectable vectors encoding the murine Ifitm proteins or with an empty vector control, as previously described [4]. MEFs were cultured for one week in puromycin to correct for variation in transduction efficiency. GFP-encoding retroviruses pseudotyped with PR8 neuraminidase and IAV HA (derived from influenza A/PR/8/34 (H1N1), influenza A/Thailand/2(SP-33)/2004 (H5N1), and A/FPV/Rostock (H7N1)) or the envelope glycoprotein of MLV or LASV were prepared as previously described [11], [28]. Ifitm-expressing MEFs were incubated with pseudoviral particles by spin-inoculation at 4000×g for 30 minutes at 4°C. Cells were washed and returned to growth medium. 48 hours later, cells were harvested, fixed in 1% paraformaldehyde, and GFP expression was quantified by flow cytometry.

Western blots

RAW264.7 cells and 3T3 cells were incubated with varying concentrations of IFNα2 (eBioscience), IFNγ2 (Antigenix America), IFNγ (Antigenix America), IL-6 (Sigma), or OSM (Sigma). Two days later, cells were lysed with 1% NP40 and lysates were analyzed by SDS-PAGE and western blot. Goat anti-mouse Ifitm3 antibody (R & D systems) and HRP-conjugated rabbit anti-goat IgG secondary antibody (Sigma) were used to detect the expression of Ifitm3. Murine anti-β-tubulin antibody (Sigma) and HRP-conjugated rabbit anti-mouse IgG secondary antibody (Santa Cruz Biotechnology) were used to measure the expression of tubulin as a loading control. Expression of myc-tagged murine Ifitms was determined using the monoclonal 9E10 antibody (Santa Cruz Biotechnology).

Flow cytometry

5×10⁵ NIH 3T3 or RAW 264.7 cells were stained with a panel of rat monoclonal IgG_2a antibodies against the OSM receptor (clone 30-1, MBL International Corporation), IL-6 receptor (clone 255821, R&D Systems), gp130 (clone 125623, R&D Systems), or an equal amount of an isotype control antibody (clone eBR2a, eBiosciences). Secondary labeling was performed with Alexa 488-conjugated donkey anti-rat (Invitrogen) and fluorescence was measured on a BD Biosceinces FACSCalibur flow cytometer.

Promoter activity assays

Approximately 800 bp of each murine promoter region was amplified from wild type MEF genomic DNA and subcloned into the XhoI and HindIII sites of the luciferase reporter vector pGL3-Enhancer (Clontech). Primers used were as follows (promoter: forward/reverse): Ifitm1: 5′-CCC CAC ATA AAA GGT CAT GG-3′/5′-TCG GCT TTT GAA GCT GCA GA-3′, Ifitm2: 5′-CTC CTC CTT GCT CCA TTC TG-3′/5′-ACT GAC TCT GGA ACA ATC GC-3′, Ifitm3: 5′-GAG TGG CTG TAG CAC CAA CA-3′/5′-GCG GAG CAA AGG CAG CAC-3′, Ifitm5: 5′-CCT CTT TGC CTG CTG TCT TC-3′/5′-TTC CAG CGC CGT GTC TTC C-3′, Ifitm6: 5′-CGA TCC TGT TTT GCC ATC TT-3′/5′-TTT GTG CTT AAA GGA AGC AAG GAA-3′, Ifitm7 5′-ATT GAG ATG GGG TTT CAC CA-3′/5′-TTG GTT TTT GAG GCT GGA AGA G-3′. NIH 3T3 cells were cultured in DMEM supplemented with 10% calf serum (Colorado Serum Company), non-essential amino acids, and penicillin/streptomycin. 3T3 cells in 12 well plates were cotransfected with 0.5 µg of the promoter/pGL3 construct and 0.5 µg of a glucokinase promoter-driven constitutive β-galactosidase reporter (pGK-β-gal) as a transfection efficiency control. 3T3 cell transfection was carried out using the TransIT-3T3 Transfection Kit (Mirus). Transfection complexes were removed after 6 hours and growth medium alone or medium containing 100 ng/mL murine IFNα2 (eBioscience) or IFNγ (Antigenix America) was added to the cells. After 24 hours of stimulation, cells were lysed and enzymatic activities quantitated by means of the commercial Luciferase Assay System and β-Galactosidase Enzyme Activity Kit (Promega) in a Victor³V plate reader (PerkinElmer). Untransfected wells were used to determine background signal. Data are presented as the background subtracted luciferase readout for each sample normalized to the background-subtracted β-galactosidase readout for that sample.

Ifitm and IFN transcript expression

NIH 3T3 and RAW 264.7 cells were grown in 12-well plates. Triplicate wells containing subconfluent cells were incubated in growth medium alone or treated for 24 hours with medium containing 500 ng/mL IFNα2, 100 ng/mL IFNγ2, 250 ng/mL OSM (3T3 cells), or 250 ng/mL IL-6 (RAW cells). RNA was isolated from cells by means of the RNeasy Mini Kit and QIAshredder columns (QIAGEN). 3T3 cells used as controls for Figure S3 were transfected with 1 5 µg/ml high molecular weight poly I:C complexed with LyoVec transfection reagent (Invivogen) for 24 hours before harvest. Primer and probe sequences for detection of murine Ifitm1, 2, and 3 transcripts follow. Ifitm1: 5′-ACC ACA ATC AAC ATG CCT GA-3′ (forward primer), 5′-CAC CAT CTT CCT GTC CCT AGA-3′ (reverse primer), and 5′-ACA CTC TTC ATG AAC TTC TGC TGC CTG-3′ (probe), Ifitm2: 5′-TTT TCT CTA CCA CCT CTG TGG T-3′ (forward primer), 5′-TGA ATC CAC TGT GGA CAG ATA G-3′ (reverse primer), and 5′-CGG TCC ACA TCT GCC CCG CC-3′ (probe), and Ifitm3: 5′-CTG AAC ATC AGC ACC TTG GT-3′ (forward primer), 5′-TTT TGG TGG TTA TCA AGT GCA CT-3′ (reverse primer), and 5′-TCC GGT CCT GAA GTG CTT CAC CCT-3′ (probe). Murine IFNβ1 was amplified with: 5′-AGA TTC ACT ACC AGT CCC AGA-3′ (forward primer), 5′-TGA AGA CCT GTC AGT TGA TGC-3′ (reverse primer), and 5′-AGG CAA CCT TTA AGC ATC AGA GGC G-3′ (probe). Murine IFNγ1 was amplified with: 5′-TCC ACA TCT ATG CCA CTT GAG-3′ (forward primer), 5′-CTG AGA CAA TGA ACG CTA CAC A-3′ (reverse primer), and 5′-TTC CTC ATG GCT GTT TCT GGC TGT-3′ (probe). Gapdh (see Viral load measurements above) was used as a housekeeping control. Primers were 5′ labeled with FAM and included 3′ IBFQ and internal Zen quencher dyes (Integrated DNA Technologies). Cycling conditions were: 50°C for 30 min (reverse transcription), 95°C for 2 min (denaturation), and 45 cycles of 95°C for 15 seconds (dentaturation) and 61.5°C for 40 sec (annealing, extension, and fluorescence acquisition). All reactions were performed in duplicate.

Immunohistochemistry, immunofluorescence, and confocal microscopy

Mouse lungs were inflated with 10% neutral buffered formalin, fixed for 24 hours, and stored in phosphate-buffered saline prior to paraffin embedding. 5 µm sections were deparaffinized and subjected to heat-mediated epitope retrieval in low pH Antigen Unmasking Solution (Vector Labs). Sections were stained for Ifitm3 (rabbit, Abcam), influenza A virus H1N1 (goat, Abcam), Mac-3 (rat IgG2a clone M3/84, BD Pharmingen), and DC-LAMP (rat IgG2a clone 1010E1.01, Imgenex). Secondary fluorescent labeling was performed with Alexa Fluor 488, 543, or 633-conjugated secondary antisera of donkey origin (Invitrogen). Ifitm-tranduced MEFs grown on 8 well chamber slides were fixed and permeabilized by immersion in a 1∶1 mixture of methanol and acetone at −20°C for 10 minutes prior to staining. Cells were stained with primary anti-myc clone 9E11 (Santa Cruz Biotechnology) and Alexa Fluor 488-conjugated goat anti-mouse secondary antibody (Invitrogen). Slides were coverslipped with ProLong Gold Antifade mounting medium containing DAPI (Invitrogen). Imaging was performed on a Leica TCS SP5 laser-scanning confocal microscope through a 63× 1.4 NA oil-immersion objective.

Brightfield immunohistochemistry for Ifitm3 was carried out with similar slide preparation and primary antibodies. Secondary labeling and detection were performed with biotinylated horse anti-rabbit, avidin/biotin/peroxidase complex, and Nova Red chromagen (Vector Labs). Digital images were color balanced for publication.

Supporting Information

Attachment 1

Attachment 2

Attachment 3

Attachment 4