The transcription and export complex THO/TREX contributes to transcription termination in plants

English version

Autoři: Ghazanfar Abbas Khan ^aff001; Jules Deforges ^aff001; Rodrigo S. Reis ^aff001; Yi-Fang Hsieh ^aff001; Jonatan Montpetit ^aff001; Wojciech Antosz ^aff003; Luca Santuari ^aff001; Christian S. Hardtke ^aff001; Klaus D. Grasser ^aff003; Yves Poirier ^aff001
Působiště autorů: Department of Plant Molecular Biology, University of Lausanne, Lausanne, Switzerland ^aff001; School of Biosciences, University of Melbourne, Melbourne, Australia ^aff002; Department of Cell Biology & Plant Biochemistry, Biochemistry Centre, University of Regensburg, Regensburg, Germany ^aff003
Vyšlo v časopise: The transcription and export complex THO/TREX contributes to transcription termination in plants. PLoS Genet 16(4): e32767. doi:10.1371/journal.pgen.1008732
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008732

Souhrn

Transcription termination has important regulatory functions, impacting mRNA stability, localization and translation potential. Failure to appropriately terminate transcription can also lead to read-through transcription and the synthesis of antisense RNAs which can have profound impact on gene expression. The Transcription-Export (THO/TREX) protein complex plays an important role in coupling transcription with splicing and export of mRNA. However, little is known about the role of the THO/TREX complex in the control of transcription termination. In this work, we show that two proteins of the THO/TREX complex, namely TREX COMPONENT 1 (TEX1 or THO3) and HYPER RECOMBINATION1 (HPR1 or THO1) contribute to the correct transcription termination at several loci in Arabidopsis thaliana. We first demonstrate this by showing defective termination in tex1 and hpr1 mutants at the nopaline synthase (NOS) terminator present in a T-DNA inserted between exon 1 and 3 of the PHO1 locus in the pho1-7 mutant. Read-through transcription beyond the NOS terminator and splicing-out of the T-DNA resulted in the generation of a near full-length PHO1 mRNA (minus exon 2) in the tex1 pho1-7 and hpr1 pho1-7 double mutants, with enhanced production of a truncated PHO1 protein that retained phosphate export activity. Consequently, the strong reduction of shoot growth associated with the severe phosphate deficiency of the pho1-7 mutant was alleviated in the tex1 pho1-7 and hpr1 pho1-7 double mutants. Additionally, we show that RNA termination defects in tex1 and hpr1 mutants leads to 3’UTR extensions in several endogenous genes. These results demonstrate that THO/TREX complex contributes to the regulation of transcription termination.

Klíčová slova:

Arabidopsis thaliana – DNA transcription – Gene expression – Messenger RNA – Phenotypes – RNA sequencing – Shoot growth – Transcriptional termination

Zdroje

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