Long noncoding RNA PAHAL modulates locust behavioural plasticity through the feedback regulation of dopamine biosynthesis

English version

Autoři: Xia Zhang ^aff001; Ya'nan Xu ^aff001; Bing Chen ^aff001; Le Kang ^aff001
Působiště autorů: State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing, China ^aff001; Beijing Institute of Life Sciences, Chinese Academy of Sciences, Beijing, China ^aff002; CAS Center for Excellence in Biotic Interactions, University of Chinese Academy of Sciences, Beijing, China ^aff003; College of Life Sciences, Hebei University, Baoding, China ^aff004
Vyšlo v časopise: Long noncoding RNA PAHAL modulates locust behavioural plasticity through the feedback regulation of dopamine biosynthesis. PLoS Genet 16(4): e32767. doi:10.1371/journal.pgen.1008771
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008771

Souhrn

Some long noncoding RNAs (lncRNAs) are specifically expressed in brain cells, implying their neural and behavioural functions. However, how lncRNAs contribute to neural regulatory networks governing the precise behaviour of animals is less explored. Here, we report the regulatory mechanism of the nuclear-enriched lncRNA PAHAL for dopamine biosynthesis and behavioural adjustment in migratory locusts (Locusta migratoria), a species with extreme behavioral plasticity. PAHAL is transcribed from the sense (coding) strand of the gene encoding phenylalanine hydroxylase (PAH), which is responsible for the synthesis of dopamine from phenylalanine. PAHAL positively regulates PAH expression resulting in dopamine production in the brain. In addition, PAHAL modulates locust behavioral aggregation in a population density-dependent manner. Mechanistically, PAHAL mediates PAH transcriptional activation by recruiting serine/arginine-rich splicing factor 2 (SRSF2), a transcription/splicing factor, to the PAH proximal promoter. The co-activation effect of PAHAL requires the interaction of the PAHAL/SRSF2 complex with the promoter-associated nascent RNA of PAH. Thus, the data support a model of feedback modulation of animal behavioural plasticity by an lncRNA. In this model, the lncRNA mediates neurotransmitter metabolism through orchestrating a local transcriptional loop.

Klíčová slova:

DNA transcription – Gene expression – Locusts – Long non-coding RNAs – Metabolic pathways – Nymphs – Small interfering RNAs – Transcriptional control

Zdroje

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