Sycp2 is essential for synaptonemal complex assembly, early meiotic recombination and homologous pairing in zebrafish spermatocytes
Autoři:
Kazumasa Takemoto aff001; Yukiko Imai aff002; Kenji Saito aff002; Toshihiro Kawasaki aff001; Peter M. Carlton aff003; Kei-ichiro Ishiguro aff004; Noriyoshi Sakai aff001
Působiště autorů:
Department of Genetics, School of Life Science, SOKENDAI (The Graduate University for Advanced Studies), Mishima, Japan
aff001; Department of Gene Function and Phenomics, National Institute of Genetics, Mishima, Japan
aff002; Radiation Biology Center and Graduate School of Biostudies, Kyoto University, Kyoto, Japan
aff003; Department of Chromosome Biology, Institute of Molecular Embryology and Genetics, Kumamoto University, Kumamoto, Japan
aff004
Vyšlo v časopise:
Sycp2 is essential for synaptonemal complex assembly, early meiotic recombination and homologous pairing in zebrafish spermatocytes. PLoS Genet 16(2): e32767. doi:10.1371/journal.pgen.1008640
Kategorie:
Research Article
doi:
https://doi.org/10.1371/journal.pgen.1008640
Souhrn
Meiotic recombination is essential for faithful segregation of homologous chromosomes during gametogenesis. The progression of recombination is associated with dynamic changes in meiotic chromatin structures. However, whether Sycp2, a key structural component of meiotic chromatin, is required for the initiation of meiotic recombination is still unclear in vertebrates. Here, we describe that Sycp2 is required for assembly of the synaptonemal complex and early meiotic events in zebrafish spermatocytes. Our genetic screening by N-ethyl-N-nitrosourea mutagenesis revealed that ietsugu (its), a mutant zebrafish line with an aberrant splice site in the sycp2 gene, showed a defect during meiotic prophase I. The its mutation appeared to be a hypomorphic mutation compared to sycp2 knockout mutations generated by TALEN mutagenesis. Taking advantage of these sycp2 hypomorphic and knockout mutant lines, we demonstrated that Sycp2 is required for the assembly of the synaptonemal complex that is initiated in the vicinity of telomeres in wild-type zebrafish spermatocytes. Accordingly, homologous pairing, the foci of the meiotic recombinases Dmc1/Rad51 and RPA, and γH2AX signals were largely diminished in sycp2 knockout spermatocytes. Taken together, our data indicate that Sycp2 plays a critical role in not only the assembly of the synaptonemal complex, but also early meiotic recombination and homologous pairing, in vertebrates.
Klíčová slova:
Homologous recombination – Immunostaining – Nuclear staining – Spermatocytes – Telomeres – Testes – Zebrafish – Chromosome staining
Zdroje
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