BLISTER-regulated vegetative growth is dependent on the protein kinase domain of ER stress modulator IRE1A in Arabidopsis thaliana

English version

Autoři: Zheng-Hui Hong ^aff001; Tao Qing ^aff002; Daniel Schubert ^aff003; Julia Anna Kleinmanns ^aff003; Jian-Xiang Liu ^aff001
Působiště autorů: State Key Laboratory of Genetic Engineering, School of Life Sciences, Fudan University, Shanghai, China ^aff001; State Key Laboratory of Plant Physiology and Biochemistry, College of Life Sciences, Zhejiang University, Hangzhou, China ^aff002; Plant Developmental Epigenetics, Heinrich Heine University Düsseldorf, Düsseldorf, Germany ^aff003; Epigenetics of Plants, Freie Universität Berlin, Berlin, Germany ^aff004
Vyšlo v časopise: BLISTER-regulated vegetative growth is dependent on the protein kinase domain of ER stress modulator IRE1A in Arabidopsis thaliana. PLoS Genet 15(12): e32767. doi:10.1371/journal.pgen.1008563
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008563

Souhrn

The unfolded protein response (UPR) is required for protein homeostasis in the endoplasmic reticulum (ER) when plants are challenged by adverse environmental conditions. Inositol-requiring enzyme 1 (IRE1), the bifunctional protein kinase / ribonuclease, is an important UPR regulator in plants mediating cytoplasmic splicing of the mRNA encoding the transcription factor bZIP60. This activates the UPR signaling pathway and regulates canonical UPR genes. However, how the protein activity of IRE1 is controlled during plant growth and development is largely unknown. In the present study, we demonstrate that the nuclear and Golgi-localized protein BLISTER (BLI) negatively controls the activity of IRE1A/IRE1B under normal growth condition in Arabidopsis. Loss-of-function mutation of BLI results in chronic up-regulation of a set of both canonical UPR genes and non-canonical UPR downstream genes, leading to cell death and growth retardation. Genetic analysis indicates that BLI-regulated vegetative growth phenotype is dependent on IRE1A/IRE1B but not their canonical splicing target bZIP60. Genetic complementation with mutation analysis suggests that the D570/K572 residues in the ATP-binding pocket and N780 residue in the RNase domain of IRE1A are required for the activation of canonical UPR gene expression, in contrast, the D570/K572 residues and D590 residue in the protein kinase domain of IRE1A are important for the induction of non-canonical UPR downstream genes in the BLI mutant background, which correlates with the shoot growth phenotype. Hence, our results reveal the important role of IRE1A in plant growth and development, and BLI negatively controls IRE1A’s function under normal growth condition in plants.

Klíčová slova:

Arabidopsis thaliana – Gene expression – Phenotypes – Protein kinases – Ribonucleases – Root growth – Seedlings – Transcription factors

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