GPCR-mediated glucose sensing system regulates light-dependent fungal development and mycotoxin production

English version

Autoři: Thaila Fernanda dos Reis ^aff001; Laura Mellado ^aff001; Jessica M. Lohmar ^aff003; Lilian Pereira Silva ^aff001; Jing-Jiang Zhou ^aff002; Ana M. Calvo ^aff004; Gustavo H. Goldman ^aff001; Neil A. Brown ^aff005
Působiště autorů: Faculdade de Ciências Farmacêuticas, Universidade de São Paulo, Ribeirão Preto, Brazil ^aff001; Biointeractions and Crop Protection, Rothamsted Research, Hertfordshire, United Kingdom ^aff002; Key Laboratory of Green Pesticide and Agricultural Bioengineering, Ministry of Education, Guizhou University, Huaxi District, Guiyang, China ^aff003; Department of Biological Sciences, Northern Illinois University, Illinois, United States of America ^aff004; Department of Biology & Biochemistry, University of Bath, Claverton Down, Bath, United Kingdom ^aff005
Vyšlo v časopise: GPCR-mediated glucose sensing system regulates light-dependent fungal development and mycotoxin production. PLoS Genet 15(10): e32767. doi:10.1371/journal.pgen.1008419
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008419

Souhrn

Microorganisms sense environmental fluctuations in nutrients and light, coordinating their growth and development accordingly. Despite their critical roles in fungi, only a few G-protein coupled receptors (GPCRs) have been characterized. The Aspergillus nidulans genome encodes 86 putative GPCRs. Here, we characterise a carbon starvation-induced GPCR-mediated glucose sensing mechanism in A. nidulans. This includes two class V (gprH and gprI) and one class VII (gprM) GPCRs, which in response to glucose promote cAMP signalling, germination and hyphal growth, while negatively regulating sexual development in a light-dependent manner. We demonstrate that GprH regulates sexual development via influencing VeA activity, a key light-dependent regulator of fungal morphogenesis and secondary metabolism. We show that GprH and GprM are light-independent negative regulators of sterigmatocystin biosynthesis. Additionally, we reveal the epistatic interactions between the three GPCRs in regulating sexual development and sterigmatocystin production. In conclusion, GprH, GprM and GprI constitute a novel carbon starvation-induced glucose sensing mechanism that functions upstream of cAMP-PKA signalling to regulate fungal development and mycotoxin production.

Klíčová slova:

Fungal genetics – Fungal structure – G protein coupled receptors – Gene regulation – Glucose – Glucose signaling – Sexual differentiation – Aspergillus nidulans

Zdroje

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