Mushroom body subsets encode CREB2-dependent water-reward long-term memory in Drosophila

English version

Autoři: Wang-Pao Lee ^aff001; Meng-Hsuan Chiang ^aff001; Li-Yun Chang ^aff001; Jhen-Yi Lee ^aff002; Ya-Lun Tsai ^aff001; Tai-Hsiang Chiu ^aff001; Hsueh-Cheng Chiang ^aff003; Tsai-Feng Fu ^aff004; Tony Wu ^aff005; Chia-Lin Wu ^aff001
Působiště autorů: Graduate Institute of Biomedical Sciences, College of Medicine, Chang Gung University, Taiwan ^aff001; School of Medicine, College of Medicine, Chang Gung University, Taiwan ^aff002; Department of Pharmacology, National Cheng-Kung University, Taiwan ^aff003; Department of Applied Chemistry, National Chi Nan University, Taiwan ^aff004; Department of Neurology, Chang Gung Memorial Hospital, Taiwan ^aff005; Department of Biochemistry, College of Medicine, Chang Gung University, Taiwan ^aff006
Vyšlo v časopise: Mushroom body subsets encode CREB2-dependent water-reward long-term memory in Drosophila. PLoS Genet 16(8): e32767. doi:10.1371/journal.pgen.1008963
Kategorie: Research Article
doi: https://doi.org/10.1371/journal.pgen.1008963

Souhrn

Long-term memory (LTM) formation depends on the conversed cAMP response element-binding protein (CREB)-dependent gene transcription followed by de novo protein synthesis. Thirsty fruit flies can be trained to associate an odor with water reward to form water-reward LTM (wLTM), which can last for over 24 hours without a significant decline. The role of de novo protein synthesis and CREB-regulated gene expression changes in neural circuits that contribute to wLTM remains unclear. Here, we show that acute inhibition of protein synthesis in the mushroom body (MB) αβ or γ neurons during memory formation using a cold-sensitive ribosome-inactivating toxin disrupts wLTM. Furthermore, adult stage-specific expression of dCREB2b in αβ or γ neurons also disrupts wLTM. The MB αβ and γ neurons can be further classified into five different neuronal subsets including αβ core, αβ surface, αβ posterior, γ main, and γ dorsal. We observed that the neurotransmission from αβ surface and γ dorsal neuron subsets is required for wLTM retrieval, whereas the αβ core, αβ posterior, and γ main are dispensable. Adult stage-specific expression of dCREB2b in αβ surface and γ dorsal neurons inhibits wLTM formation. In vivo calcium imaging revealed that αβ surface and γ dorsal neurons form wLTM traces with different dynamic properties, and these memory traces are abolished by dCREB2b expression. Our results suggest that a small population of neurons within the MB circuits support long-term storage of water-reward memory in Drosophila.

Klíčová slova:

Calcium imaging – Conditioned response – Drosophila melanogaster – Memory – Neurons – Neurotransmission – Olfactory receptor neurons – Protein synthesis

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