Imunomodulační aktivita etanolových extraktů z Galium verum L. herb.

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Autoři: Igor L. Shinkovenko; Natalia V. Kashpur; Tetiana V. Ilina; Alla M. Kovalyova; Olga V. Goryacha; Oleh M. Koshovyi; Olena V. Kryvoruchko; Andryi M. Komissarenko
Vyšlo v časopise: Čes. slov. Farm., 2018; 67, 101-106
Kategorie: Původní práce

Souhrn

V tomto článku jsou diskutovány výsledky výzkumu fytochemického profilu a imunomodulační aktivity etanolických extraktů Galli veri herba (Galium verum L., Rubiaceae). Studované extrakty se získaly z rostlinného materiálu macerací za tepla 20% vodným roztokem ethanolu (tekutý extrakt I), 60% vodným roztokem ethanolu (tekutý extrakt II) a 96% ethanolem (tekutý extrakt III). Obsah hydroxycinnamických derivátů, flavonoidů a polyfenolů byl stanoven spektrofotometricky; polysacharidy byly kvantifikovány gravimetricky; imunomodulační aktivita studovaných látek byla stanovena reakcí blastické transformace lymfocytů (RLBT). Bylo zjištěno, že tekutý extrakt I obsahoval 2,4 % polysacharidů, 3,1 % hydroxycinnamických derivátů, 0,24 % flavonoidů a 2,9% polyfenolů; tekutý extrakt II obsahoval 4,13 % hydroxycinnamových derivátů, 0,16 % flavonoidů a 3,84 % polyfenolů; tekutý extrakt III obsahoval 2,7 % hydroxycinnamických derivátů, 0,18 % flavonoidů a 2,7 % polyfenolů. Všechny studované extrakty vykazovaly výrazný stimulační účinek na transformační aktivitu imunokompetentních krevních buněk. Nejvyšší imunomodulační aktivita byla stanovena pro 96% ethanolový extrakt: procento lymfocytů proliferujících v RLBT vlivem tohoto extraktu se zvýšilo o 6,77–8,04krát ve srovnání s jejich spontánní transformací a 1,14–1,36krát ve srovnání s fytohemaglutininem. Získané výsledky dávají důvody pro další výzkum mechanizmů imunomodulační aktivity extraktů z natě G. verum.

Klíčová slova: ethanolické extrakty • Galium verum L. • imunomodulační aktivita • blastická transformace lymfocytů

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