Inhibícia 12/15 lipoxygenázy kurkumínom a extraktom z Curcuma longa L.

English version

Autoři: Lýdia Bezáková; Daniela Košťálová; Marek Obložinský; Peter Hoffman; Mária Pekárová; Renáta Kollárová; Ivana Holková; Silvia Mošovská; Ernest Šturdík
Působiště autorů: Comenius University, Faculty of Pharmacy
Vyšlo v časopise: Čes. slov. Farm., 2014; 63, 26-31
Kategorie: Původní práce

Souhrn

Kurkumín (diferuloylmetan) je oranžovo-žlto sfarbená obsahová látka – sekundárny metabolit z rizomy (Curcuma longa L.), korenia (turmeric) často prítomného v prášku curry. Je to jeden z hlavných kurkuminoidov turmeric Curcuma longa L. Po stáročia bol kurkumín používaný v niektorých liečivých preparátoch, alebo ako korigens farby v potravinách. Ukázalo sa, že viaceré enzýmy, ktoré sú úzko spojené so zápalovým a nádorovým procesom, sú modulované kurkumínom. V tejto práci sú sumarizované výsledky inhibičného efektu kurkumínu a etanolického extraktu z Curcuma longa L. na aktivitu lipoxygenázy (LOX) z cytozolovej frakcie pľúc potkana. Stanovenie polohovej špecifity dioxygenácie kyseliny arachidónovej RP- and SP-HPLC metódou ukázalo, že v purifikovanom enzýmovom preparáte z cytozolovej frakcie pľúc potkana je prítomná špecifická forma lipoxygenázy preukazujúca 12/15-LOX duálnu špecifitu (s prevažujúcou 15-LOX aktivitou). Inhibičná aktivita kurkumínu a extraktu z Curcuma longa na aktivitu lipoxygenázy z cytozolovej frakcie pľúc potkana bola vyjadrená v percentách inhibície a hodnotách IC₅₀. Vyjadrením kinetiky enzýmovej reakcie podľa Lineweaver-Burk za prítomnosti inhibítora sa zistilo, že kurkumín je kompetitívny inhibítor 12/15- LOX z cytozolovej frakcie pľúc potkana.

Keywords:
Curcuma longa L., kurkumín, turmeric extrakt, kyselina linolová, inhibícia lipoxygenázy

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